This web page was produced as an assignment for an undergraduate course at Davidson College.

PCR Primers for 5 Yeast (Saccharomyces cerevisiae) IDH Genes

IDH1 Forward: 5' ATGCTTAACAGAACAATTGC 3' T_m = 46° C

IDH1 Reverse: 5' TTACATGGTAGATAATTTGTTG 3' T_m = 46° C

IDH2 Forward: 5' ATGTTGAGAAATACTTTTTTTTAG 3' T_m = 45° C

IDH2 Reverse: 5' TTATAATCTCTTGATGACTG 3' T_m = 44° C

IDP1 Forward: 5' ATGAGTATGTTATCTAGAAG 3' T_m = 44° C

IDP1 Reverse: 5' TTACTCGATCGACTTGATTT 3' T_m = 46° C

IDP2 Forward: 5' ATGACAAAGATTAAGGTAGC 3' T_m = 46° C

IDP2 Reverse: 5' TTACAATGCAGCTGCCTCGA 3' T_m = 52° C

IDP3 Forward: 5' ATGAGTAAAATTAAAGTTGTTCAT 3' T_m = 45° C

IDP3 Reverse: 5' TTATAGTTTGCACATACCTT 3' T_m = 44° C

This page is maintained by Nicole Hesson.