This web page was produced as an assignment for an undergraduate course at Davidson College.

PCR Primers for 5 Yeast (Saccharomyces cerevisiae) IDH Genes


IDH1 Forward: 5' ATGCTTAACAGAACAATTGC 3' Tm = 46° C

IDH1 Reverse: 5' TTACATGGTAGATAATTTGTTG 3' Tm = 46° C

 

IDH2 Forward: 5' ATGTTGAGAAATACTTTTTTTTAG 3' Tm = 45° C

IDH2 Reverse: 5' TTATAATCTCTTGATGACTG 3' Tm = 44° C

 

IDP1 Forward: 5' ATGAGTATGTTATCTAGAAG 3' Tm = 44° C

IDP1 Reverse: 5' TTACTCGATCGACTTGATTT 3' Tm = 46° C

 

IDP2 Forward: 5' ATGACAAAGATTAAGGTAGC 3' Tm = 46° C

IDP2 Reverse: 5' TTACAATGCAGCTGCCTCGA 3' Tm = 52° C

 

IDP3 Forward: 5' ATGAGTAAAATTAAAGTTGTTCAT 3' Tm = 45° C

IDP3 Reverse: 5' TTATAGTTTGCACATACCTT 3' Tm = 44° C

 

This page is maintained by Nicole Hesson.

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