Saccharomyces cervesisiae ORF: YEL068C

Despite the complete sequencing of Saccharomyces' genome, not all genes have a known protein product and function. YEL068C is an ORF (open reading frame) located on chromosome 5 from 25646 to 25978 kb (SGD, 2001).

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Mini ORF map of YEL068C (permission requested from SGD curators)

YEL068C has a 332 kb nucleotide sequence (SGD, 2001):

1 ATGAAAATTT TGGCACTAGC CGTCGCCTTT CAAGGCTTGG CATTGGTATG

51 CTGTACGGAT ACAACAGACA ATTATAACAA TTTTACGCAT AATACATCGA

101 CATTTTACCG CATAGCGGAT TATGATAAGA GATTTTGGAG CGTAAAACTA

151 CCACCAGTGA ATTGGACTAC TTGCGGTAAT GAAGATCATC CATGGATTAC

201 TATCCACTAC GACGAGATTG ATAAACTCGT TGACGATAAA CTGGCAAGTT

251 GGGACAAAGG ACCTGTTCCC GATCGTGAAG AATTTAGGAA CTCCATCCTT

301 AGACAAGCGA GATGCGACCG CCCACATTAC TAG

The transcribed amino acid sequence (SGD, 2001):

LVMWAVASRLSKDGVPKFFTIGNRSFVPTCQFIVNEFINLVVVDSNPWMIFITASSPIHW
W*FYAPKSLIIIRYAVKCRCIMRKIVIIVCCIRTAYQCQALKGDG*CQNFH

 

BLAST(N) Accession # NC_001137 and GI 7276232

No sequence orthologs for YEL068C

NCBI CD search reveals no hits for Conserved Domain of YEL068C's amino acid sequence.

A PREDATOR search predicts 34% Alpha Helix, 22.94% Extended Strand, and Random Coil 42.20%.

 
      10        20        30        40        50        60        70
         |         |         |         |         |         |         |
LVMWAVASRLSKDGVPKFFTIGNRSFVPTCQFIVNEFINLVVVDSNPWMIFITASSPIHWWFYAPKSLII
ccchhhhhhhhcccccceeeecccccccceeeeecccceeeeeccccceeeeeecccceeeeecccchhh
IRYAVKCRCIMRKIVIIVCCIRTAYQCQALKGDGCQNFH
hhhhhhhcchhhhhhhchhhhhhhhhhhhhccccccccc
Sequence length :   109
PREDATOR :
   Alpha helix     (Hh) :    38 is  34.86%
   3
10  helix       (Gg) :     0 is   0.00%
   Pi helix        (Ii) :     0 is   0.00%
   Beta bridge     (Bb) :     0 is   0.00%
   Extended strand (Ee) :    25 is  22.94%
   Beta turn       (Tt) :     0 is   0.00%
   Bend region     (Ss) :     0 is   0.00%
   Random coil     (Cc) :    46 is  42.20%
   Ambigous states (?)  :     0 is   0.00%
   Other states         :     0 is   0.00%

A Kyte-Doolitte Analysis of the amino acid sequence revealed that the later section produces a hit of more than two and thus maybe a part of a transmembrane domain.

permission requested

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