This web page was produced as an assignment for an undergraduate course at Davidson College.
Background
review:
As
stated in my second web assignment, the BUD8 gene
is specifically involved in the bud initiation of bipolar budding.
It is localized to the distal pole of the growing daughter cell and
the mother side of the mother-bud neck in both haploid and diploid yeast form
cells. It is also involved in the
formation of pseudohyphal filaments during the budding process.
Scale : (fold repression/induction)
Experiment 1: BUD8 expression at different
alpha-factor concentrations
Orf |
Gene |
|
Process |
Function |
Component |
BUD8 |
pseudohyphal growth* |
molecular_function unknown |
cell |
||
SIR3 |
chromatin silencing at HML and HMR (sensu Saccharomyces)* |
not yet annotated |
nucleolus* |
||
PSD2 |
not yet annotated |
phosphatidylserine decarboxylase |
not yet annotated |
||
NUP1 |
mRNA-nucleus export* |
structural protein |
nuclear pore |
||
POX1 |
fatty acid beta-oxidation |
acyl-CoA oxidase |
peroxisomal matrix |
This experiment was primarily profiling the genome wide transcription reponse
to monitor signal transduction during the yeast response to pheromone.
In this case, BUD8 shows similar expression patterns as such genes
as SIR3 and NUP1. These genes don’t seem to share much of the
same function as BUD8. SIR3 is involved
in cell aging and chromatin silencing at the telomere, and NUP1 is a structural
protein. However, it is interesting
to note that SIR3 is also located on chromosome XII with BUD8. http://www.rii.com/tech/pubs/s287873.htm
Orf |
Gene |
|
Process |
Function |
Component |
BUD8 |
pseudohyphal growth* |
molecular_function unknown |
cell |
||
CDC47 |
DNA replication initiation* |
chromatin binding* |
cytoplasm* |
||
PHO4 |
not yet annotated |
transcription factor |
not yet annotated |
||
ALR1 |
di-, tri-valent inorganic
cation transport |
di-, tri-valent inorganic
cation transporter |
plasma membrane |
||
NAR1 |
not yet annotated |
molecular_function unknown |
not yet annotated |
||
HMS2 |
pseudohyphal growth |
transcription factor |
not yet annotated |
||
ALF1 |
post-chaperonine tubulin
folding pathway* |
cochaperone |
microtubule |
||
MRS3 |
transport* |
carrier |
mitochondrion |
Expression 3: BUD8 Expression during the cell cycle
BUD8
compared to reference genes during the cell cycle.
Name |
Score |
Peak |
|
4.286 |
M |
||
Reference Genes |
|
||
10.9 |
G1 |
||
10.68 |
S |
||
3.08 |
G2 |
||
6.726 |
M |
||
11.8 |
M/G1 |
||
|
|
|
|
BUD8 compared to 20 different genes
with similar expression during the cell cycle.
Name |
Score |
Peak |
|
4.286 |
M |
|
|
5.674 |
M |
|
|
1.5085 |
M |
|
|
8.396 |
M |
|
|
10.97 |
M |
|
|
9.908 |
M |
|
|
5.693 |
M |
|
|
7.306 |
M |
|
|
7.36 |
M |
|
|
9.901 |
M |
|
|
6.726 |
M |
|
|
10.65 |
M |
|
|
4.456 |
M |
|
|
5.242 |
M |
|
|
5.369 |
G2 |
|
|
6.092 |
M |
|
|
6.421 |
M |
|
|
5.613 |
M |
|
|
1.603 |
M |
|
|
3.017 |
G2 |
|
|
1.721 |
M |
|
During the cell cycle, BUD8 is repressed and induced in varying degrees with a peak in the mitosis phase of the cycle. The four grouping represent the BUD8 expression to different pheromones. The BUD8 gene appears to be consistently repressed at the beginning of each cycle with a gradual induction similar to the SWI5 transcriptional activator to which BUD8 was compared. When clustered with 20 genes of similar expression, the BUD3 and BUD4 genes have the closest score to BUD8. The BUD3 and BUD4 genes are also involved in bud site selection and they co-assemble at the bud sites.
Experiment 4: BUD8 Expression in response to environmental changes
Orf |
|
Gene |
|
|
|
Process |
|
Function |
|
Component |
|
BUD8 |
|
|
|
|
|
|
|
In response to environmental changes, BUD8 did not show a similar expression pattern to any other genes.
Orf |
|
Gene |
|
|
|
Process |
|
Function |
|
Component |
|
BUD8 |
|
|
pseudohyphal growth* |
|
molecular_function unknown |
|
cell |
||
|
FIL1 |
|
|
protein biosynthesis |
|
translation termination
factor |
|
mitochondrion |
||
|
MRP51 |
|
|
protein biosynthesis* |
|
structural protein of
ribosome |
|
mitochondrial small
ribosomal subunit |
||
|
TIM44 |
|
|
mitochondrial translocation |
|
protein transporter |
|
mitochondrial matrix |
||
|
MRPS5 |
|
|
protein biosynthesis |
|
structural protein of
ribosome |
|
mitochondrial small
ribosomal subunit* |
||
|
MBA1 |
|
|
respiration |
|
not yet annotated |
|
not yet annotated |
||
|
AGE1 |
|
|
ER to Golgi transport* |
|
ARF GTPase activator |
|
cellular_component unknown |
||
|
GSH1 |
|
|
not yet annotated |
|
glutamate--cysteine ligase |
|
not yet annotated |
||
|
GLE2 |
|
|
mRNA-nucleus export* |
|
structural protein |
|
nuclear pore |
||
|
SRP40 |
|
|
nucleocytoplasmic transport |
|
chaperone |
|
nucleolus |
||
|
YMR31 |
|
|
protein biosynthesis |
|
structural protein of
ribosome |
|
mitochondrial small
ribosomal subunit |
||
|
KGD1 |
|
|
tricarboxylic acid cycle* |
|
oxoglutarate dehydrogenase
(lipoamide) |
|
mitochondrial matrix |
||
|
AYR1 |
|
|
phosphatidic acid biosynthesis |
|
acylglycerone-phosphate
reductase |
|
endoplasmic reticulum* |
SUMMARY:
BUD8 does not appear to have similar expression patterns
with the same genes throughout the microarray experiments. However this data does help characterize the
expression pattern of BUD8 and it shows us that there are many complex processes
occurring simultaneously in the life of a yeast.
REFERENCES:
http://www.rii.com/tech/pubs/s287873.htm, accessed October 17, 2001.
http://genome-www4.stanford.edu/cgi-bin/SGD/locus.pl?locus=YJR147W,
accessed October 17, 2001.
http://genome-www4.stanford.edu/cgi-bin/SGD/expression/expressionConnection.pl,
accessed October 17, 2001.
EMAIL ME AT mrwilson@davidson.edu