LOCUS
NM_012624 3160 bp mRNA
ROD 12-FEB-2000
DEFINITION Rattus norvegicus Pyruvate
kinase, liver and RBC (Pklr), mRNA.
ACCESSION NM_012624
VERSION NM_012624.1
GI:6981359
KEYWORDS .
SOURCE Norway
rat.
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia;
Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to
3160)
AUTHORS Inoue,H.,
Noguchi,T. and Tanaka,T.
TITLE
Complete amino acid sequence of rat L-type pyruvate kinase deduced
from the cDNA sequence
JOURNAL Eur. J. Biochem.
154 (2), 465-469 (1986)
MEDLINE 86108360
REFERENCE 2 (bases 235 to
3160)
AUTHORS Lone,Y.C.,
Simon,M.P., Kahn,A. and Marie,J.
TITLE
Complete nucleotide and deduced amino acid sequences of rat L-type
pyruvate kinase
JOURNAL FEBS Lett.
195 (1-2), 97-100 (1986)
MEDLINE 86108907
REFERENCE 3 (bases 1742
to 3160)
AUTHORS Marie,J.,
Simon,M.P., Lone,Y.C., Cognet,M. and Kahn,A.
TITLE
Tissue-specific heterogeneity of the 3'-untranslated region of
L-type pyruvate kinase mRNAs
JOURNAL Eur. J. Biochem.
158 (1), 33-41 (1986)
MEDLINE 86274682
REFERENCE 4 (bases 1 to
3160)
AUTHORS Sakai,H. and
Ohta,T.
TITLE
Molecular cloning and nucleotide sequence of the gene for pyruvate
kinase of Bacillus stearothermophilus and the production of the
enzyme in Escherichia coli. Evidence that the genes for
phosphofructokinase and pyruvate kinase constitute an operon
JOURNAL Eur. J. Biochem.
211 (3), 851-859 (1993)
MEDLINE 93170322
COMMENT REFSEQ: This
reference sequence was derived from X03430.1.
PROVISIONAL RefSeq: This is a provisional reference sequence record
that has not yet been subject to human review. The final curated
reference sequence record may be somewhat different from this one.
FEATURES
Location/Qualifiers
source
1..3160
/organism="Rattus norvegicus"
/db_xref="taxon:10116"
/chromosome="2"
/map="2"
gene
1..3160
/gene="Pklr"
/note="PK1; PKL; Pklg"
/db_xref="LocusID:24651"
/db_xref="RATMAP:34290"
conflict
4
/note="G is C in [2]"
/citation=[2]
CDS
251..1882
/gene="Pklr"
/codon_start=1
/product="pyruvate kinase, liver and RBC"
/protein_id="NP_036756.1"
/db_xref="GI:6981360"
/translation="MEGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCL
LDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIAN
IREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKF
QTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSR
KGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQN
IKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGK
PVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHA
IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSA
QLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIES
GKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS"
misc_feature
284..286
/note="phosphorylatable site [2]"
conflict
1649
/note="C is G in [2]"
/citation=[2]
conflict
1658
/note="C is A in [2]"
/citation=[2]
conflict
1886
/note="C is G in [3]"
/citation=[3]
conflict
1928
/note="U is C in [2] and [3]"
/citation=[2]
/citation=[3]
conflict
2018
/note="G is missing in [2] and [3]"
/citation=[2]
/citation=[3]
polyA_site
2028
/note="polyA site (2kb mRNA) [3]"
conflict
2179
/note="U is C in [2] and [3]"
/citation=[2]
/citation=[3]
polyA_site
2212
/note="polyA site (2.2kb mRNA) [3]"
polyA_site
2225
/note="alt. polyA site (2.2kb mRNA) [3]"
repeat_region
2354..2393
/note="Alu-like repetitive sequence [2]"
misc_feature
2394..2476
/note="cID sequence complementary to [2] identifier
sequence"
repeat_region
2476..2593
/note="Alu-like repetitive sequence [2]"
misc_feature
3131..3136
/note="polyadenylation signal [2]"
polyA_site
3160
/note="polyA site (3.2kb mRNA) [2]"
BASE COUNT 763
a 833 c 821 g 743
t
ORIGIN
1 atcttggaaa acgaccccac ggaacttgac ctcatgttct gcatagatga agagaacttt
61 gggcagactt accaagtgga cctgaagccc aatgggtcag aaatcatggt aaccaatgag
121 aacaagcgag
aatatattga cttggtcatc cagtggagat ttgtgaacag ggtccagaag
181 caaatgaatg
ccttcttgga gggatttaca gaactgaagt ttgatgaaat tctagaagca
241 acgtagcagc
atggaagggc cagcgggata ccttcgacgt gcgagtgtgg ctcaactgac
301 ccaggagctg
ggcactgcct tcttccagca gcagcaactg cccgcagcta tggcggacac
361 cttcctggaa
cacctctgcc ttctggatat cgactcacag cctgtggctg ctcgtagcac
421 cagcatcatt
gccaccattg ggccagcatc ccgctctgtg gaccgcctca aggagatgat
481 caaagcaggg
atgaacattg cacgactcaa cttctcccat ggctcccatg agtaccatgc
541 agaatccatc
gccaacatcc gggaggcaac tgagagtttt gcaacctccc cactcagcta
601 cagacctgtg
gccatcgccc tggacaccaa gggacctgag atacgaaccg gagtcttgca
661 ggggggtccg
gagtcggagg tggaaattgt gaagggctca caggtgctgg tgacggtgga
721 cccgaagttc
cagacaaggg gtgatgcaaa gacagtgtgg gtggactacc acaatatcac
781 ccgggtcgtt
gcagtggggg gccgcatcta cattgacgac gggctcatct ccttagtggt
841 acagaaaatc
ggcccagagg gactggtgac agaagtggag cacggtggta tcttgggcag
901 caggaagggt
gtgaacttgc caaacactga ggtggacctg cccgggctgt ctgagcaaga
961 ccttttggat
ctgcgcttcg gggtgcagca taatgtggac atcatctttg cctcctttgt
1021 gcggaaagcc
agtgacgtgt tagcagtccg ggatgccctg gggccagaag gacagaacat
1081 caaaattatc
agcaaaatcg agaaccatga aggcgtgaag aagtttgatg aaattctaga
1141 agtgagcgat
ggcatcatgg tggcacgggg tgacctgggc attgagatcc ctgcggagaa
1201 ggttttcttg
gctcagaaga tgatgattgg acgctgcaac ctggccggca agcctgtcgt
1261 ttgtgccaca
cagatgctgg agagcatgat cactaaggct cgaccaactc gggcggagac
1321 aagcgatgtg
gccaatgccg tgctggatgg ggctgactgt atcatgctgt ccggagagac
1381 cgccaagggc
agttttcctg tggaagctgt aatgatgcaa catgcgattg cgcgggaggc
1441 agaggccgct
gtgtaccacc gccagttgtt tgaggagcta cgccgggcag cgccgctgag
1501 ccgtgaccca
actgaggtca ctgcgattgg agccgtggag gcttccttca agtgctgtgc
1561 agcagccatc
atcgtgctga cgaagactgg ccgttcagcc cagcttctat ctcaataccg
1621 acctcgggcg
gctgtcattg ctgtgactcg atctgcccag gctgcccgac aggtccacct
1681 gtcccgagga
gtcttcccct tgctctaccg tgagcctcca gaggccatct gggcagatga
1741 tgtggatcga
agggtccaat ttggcattga aagtggaaag ctccgtggtt tcctccgtgt
1801 gggtgatctg
gtgattgtgg tgacaggttg gcggcctggc tctggctata ccaacatcat
1861 gcgggtgctg
agcgtatcct gaaatccctc tccccattct gacccagtta caccctattt
1921 ctttcaatcc
acacccctcc catagtccta catctgccat ctagccccat ccctgtgctt
1981 tacacaggcc
ctgaatgtct gtgtccaatt atacagtggc caccggcagc atcggttgta
2041 tatccctgtc
tcaatccgct cagctggact ctaagatacc ctgagccttt aatcccagcc
2101 cagctggttg
attcgattcc ttccgggtcc caatcattgg aatgggggag tggaaacagg
2161 gtgatcttgt
ccaattttta tacaatcatg attttaaaac actgtctgat ataaccctca
2221 tgatcagttt
cctagcaaag tgtcatctcc taatggcctc aagtcagggc agaatactcc
2281 ttcaaggagc
acagctccac actttaggga aggctggggc agctgggtac tggagagaac
2341 taagacaggc
tggcttttct ctctctctct tttttttttt ttcttttctt tttctttttt
2401 tcggagctgg
ggaccgaacc cagggcattg tgttgctagg caagcgctct accactgagc
2461 taaatcccca
accccagctt ttctcttttt aatacaagct ctcactggcc tcaaactcct
2521 aagtcctcct
gcctggccct cctaagggta gggactacag gcatgagtga ccagctggac
2581 ttcgggtagc
cttattttct tactgactcc acaaaccatg gttgttctcc tgcccactgc
2641 tctgctgggt
cagatgatcc agaaattctt ccacaaccac ttggctccca catacaaatt
2701 agaagcaaaa
ctgaatcttt tcttttaaac ccaactgttt aggtgcaatt ataaaaacaa
2761 ctccacaggc
aaagaatccc agaatctcct accctaggag atgtatagtc ctggccccac
2821 ccatcaatgc
tgtagtatac tcctgaagcg ggacagaact ggtggacagg ggactcctct
2881 tgtccctaag
aaagtggagg cactgttggc ccacccctcc taggtttgaa tactccaggc
2941 cctcctcttc
agcaccaaca gcaaatccag atgagaaaaa aaaaataagt gcagttctcc
3001 tgctgccctc
ctcttttcac tacctcaata cagcaagttt gagtattgct gctgatggca
3061 gtgtgcaagg
accacaaaga tgtcccccct cagcccccta ccagaaggtg gagaggacag
3121 aggaatgaat
aataaagtga atgcgtcaaa ttagcaaatg
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Department of Biology, Davidson College, Davidson, NC 28036.
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