This web page was produced as an assignment for an
undergraduate course at Davidson College.
MRPL28 Gene in Saccharomyces cerevisiae
Note: My conclusions are displayed directly below each microarray data
set, and final conclusions located at the bottom of the page.
Databases Searched
![[X]](http://genome-www.stanford.edu/images/right.gif)
Expression at different
alpha-factor concentrations (Rosetta Inpharmatics).
|
Expression at different alpha-factor concentrations
for MRPL28/YDR462W
|
 |
Scale : (fold repression/induction)
Click on a color strip to see data for that gene.
Up to 20 similar genes are shown, with a Pearson correlation of > 0.8 to
the query gene
| Orf |
|
Gene |
|
 |
|
Process |
|
Function |
|
Component |
| YDR462W |
|
MRPL28 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial large ribosomal subunit |
| YOR389W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YLR006C |
|
SSK1 |
|
 |
|
osmosensory signaling pathway via two-component
system |
|
enzyme activator* |
|
cellular_component unknown |
| YPL270W |
|
MDL2 |
|
 |
|
transport |
|
not yet annotated |
|
not yet annotated |
| YNL215W |
|
IES2 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YOR022C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YGL220W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YER172C |
|
BRR2 |
|
 |
|
lariat formation, 5'-splice site cleavage |
|
ATP dependent RNA helicase* |
|
snRNP U5e* |
| YHL021C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YLR306W |
|
UBC12 |
|
 |
|
polyubiquitylation* |
|
ubiquitin conjugating enzyme |
|
cellular_component unknown |
| YPL099C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YLR230W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YGR246C |
|
BRF1 |
|
 |
|
transcription initiation, from Pol III promoter |
|
RNA polymerase III transcription factor |
|
transcription factor TFIIIB |
| YGL107C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YGR169C |
|
PUS6 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YIR003W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YOR154W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YAL009W |
|
SPO7 |
|
 |
|
meiosis |
|
molecular_function unknown |
|
not yet annotated |
| YMR052W |
|
FAR3 |
|
 |
|
pheromone response (sensu Saccharomyces)* |
|
molecular_function unknown |
|
cellular_component unknown |
| YJR002W |
|
MPP10 |
|
 |
|
rRNA modification* |
|
small nuclear ribonucleoprotein |
|
small nucleolar ribonucleoprotein particle |
| YJL197W |
|
UBP12 |
|
 |
|
deubiquitylation |
|
ubiquitin-specific protease |
|
cellular_component unknown |
* : indicates that more than one annotation exists for the gene.
SGDtm pages Database Copyright © 1997-2001 The Board
of Trustees of Leland Stanford Junior University. Permission to use the
information contained in this database was given by the researchers/institutes
who contributed or published the information. Users of the database are solely
responsible for compliance with any copyright restrictions, including those
applying to the author abstracts. Documents from this server are provided
"AS-IS" without any warranty, expressed or implied.
Search
another gene name | Go to list of Databases Searched
| SGD Home
The data set from the different Alpha factor concentrations has very little information contained in it. It is impossible to deduce anything from
this microarray, because the amount of change in the expression of MRPL28 is almost zero. However, the genes to which
MRPL28 is compared show much variability in their expression. This gives
rise to questions about the clustering algorithm used, because the colors do not
match up very well for the genes clustered together. Most of the genes are involved in some aspect of
transcription, though, which makes sense. Because MRPL28 is involved in protein
biosynthesis, it should cluster with other genes that involve transcription. Overall, though, it appears that the type of reproduction that the yeast undergoes
(the stage in the life cycle of yeast) does not affect the expression of MRPL28.
Expression in response to
alpha-factor (Rosetta Inpharmatics).
|
|
Expression in response to alpha-factor for
MRPL28/YDR462W
|
|
Scale : (fold repression/induction)
Click on a color strip to see data for that gene.
Up to 20 similar genes are shown, with a Pearson correlation of > 0.8 to
the query gene
| Orf |
|
Gene |
|
 |
|
Process |
|
Function |
|
Component |
| YDR462W |
|
MRPL28 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial large ribosomal subunit |
| YHR134W |
|
WSS1 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YDR188W |
|
CCT6 |
|
 |
|
protein folding* |
|
chaperone |
|
cytoplasm* |
| YLR317W |
|
KRE34 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YDR040C |
|
ENA1 |
|
 |
|
transport |
|
not yet annotated |
|
not yet annotated |
| YMR255W |
|
GFD1 |
|
 |
|
mRNA-nucleus export |
|
molecular_function unknown |
|
cytoplasm* |
| YML113W |
|
DAT1 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YBR045C |
|
GIP1 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YMR146C |
|
TIF34 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YBL071C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YNL097C |
|
PHO23 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YKL125W |
|
RRN3 |
|
 |
|
transcription, from Pol I promoter |
|
RNA polymerase I transcription factor |
|
RNA polymerase I transcription factor complex |
| YLR426W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YMR143W |
|
RPS16A |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
cytosolic small ribosomal (40S) subunit |
| YBR206W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YNR046W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YPR011C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YDR185C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YAL046C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YLR072W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YAL013W |
|
DEP1 |
|
 |
|
phospholipid metabolism |
|
not yet annotated |
|
not yet annotated |
* : indicates that more than one annotation exists for the gene.
SGDtm pages Database Copyright © 1997-2001 The Board
of Trustees of Leland Stanford Junior University. Permission to use the
information contained in this database was given by the researchers/institutes
who contributed or published the information. Users of the database are solely
responsible for compliance with any copyright restrictions, including those
applying to the author abstracts. Documents from this server are provided
"AS-IS" without any warranty, expressed or implied.
Search
another gene name | Go to list of Databases Searched
| SGD Home
As is shown above, MRPL28 shows very little response to the Alpha factor. However, it is again clustered with genes with biological processes that
involve transcription, which is expected. The clustering algorithm seems to be
better in this array set, as the different genes in the set show the same
general trend of initial stability, followed by a slight repression and then
more significant induction. However, the amount of either repression or
induction shown by MRPL28 is small, and drastic conclusions based on this array
must not be made, as this could be just noise.
Expression in response to
DNA-damaging agents (Stanford University).
|
|
Expression in response to DNA-damaging agents for
MRPL28/YDR462W
|
|
Scale : (fold repression/induction)
Click on a color strip to see data for that gene.
Up to 20 similar genes are shown, with a Pearson correlation of > 0.8 to
the query gene
| Orf |
|
Gene |
|
 |
|
Process |
|
Function |
|
Component |
| YDR462W |
|
MRPL28 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial large ribosomal subunit |
* : indicates that more than one annotation exists for the gene.
Expression in response to DNA-damaging agents for MRPL28/YDR462W
Visit the Website |
|
SGDtm pages Database Copyright © 1997-2001 The Board
of Trustees of Leland Stanford Junior University. Permission to use the
information contained in this database was given by the researchers/institutes
who contributed or published the information. Users of the database are solely
responsible for compliance with any copyright restrictions, including those
applying to the author abstracts. Documents from this server are provided
"AS-IS" without any warranty, expressed or implied.
Search
another gene name | Go to list of Databases Searched
| SGD Home
MRPL28 again shows nearly no response to the stresses put upon it in these microarrays.
The amount of either fold repression or induction is slight, and few conclusions
can be drawn. The only spots out of the many in these microarrays that show real deviation from the control
expression are those involving the mec1 mutant. It is shown that gamma
irradiation has an effect on the MRPL28 gene in mec1 mutants, but not in the
wild type strain. Therefore, it could be assumed that there is a difference in
the MRPL28 gene in mec1 mutants versus wild type cells, and further research
could isolate this difference.
Expression during the
diauxic shift (Stanford University).
|
|
Expression during the diauxic shift for
MRPL28/YDR462W
|
|
Scale : (fold repression/induction)
Click on a color strip to see data for that gene.
Up to 20 similar genes are shown, with a Pearson correlation of > 0.8 to
the query gene
| Orf |
|
Gene |
|
 |
|
Process |
|
Function |
|
Component |
| YDR462W |
|
MRPL28 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial large ribosomal subunit |
| YKL002W |
|
DID4 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YKL087C |
|
CYT2 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YNL051W |
|
COD4 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YPR166C |
|
MRP2 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial small ribosomal subunit |
| YMR284W |
|
YKU70 |
|
 |
|
DNA repair |
|
not yet annotated |
|
not yet annotated |
| YGL004C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YJL170C |
|
ASG7 |
|
 |
|
mating (sensu Saccharomyces) |
|
molecular_function unknown |
|
cellular_component unknown |
| YKL003C |
|
MRP17 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial small ribosomal subunit |
| YKL169C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YJL042W |
|
MHP1 |
|
 |
|
cell wall organization and biogenesis* |
|
structural protein of cytoskeleton |
|
microtubule |
| YHR116W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YPL013C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
cellular_component unknown |
| YDL193W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
endoplasmic reticulum* |
| YNL073W |
|
MSK1 |
|
 |
|
not yet annotated |
|
lysine--tRNA ligase |
|
not yet annotated |
| YGR219W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YBR177C |
|
EHT1 |
|
 |
|
lipid metabolism |
|
molecular_function unknown |
|
lipid particle |
| YMR157C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YMR158W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YNL122C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YLR254C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
* : indicates that more than one annotation exists for the gene.
SGDtm pages Database Copyright © 1997-2001 The Board
of Trustees of Leland Stanford Junior University. Permission to use the
information contained in this database was given by the researchers/institutes
who contributed or published the information. Users of the database are solely
responsible for compliance with any copyright restrictions, including those
applying to the author abstracts. Documents from this server are provided
"AS-IS" without any warranty, expressed or implied.
Search
another gene name | Go to list of Databases Searched
| SGD Home
The MRPL28 gene showed a slight initial repression of expression
followed by a more drastic induction in the diauxic shift microarray.
The change from anaerobic to aerobic respiration seemed to have some influence
on the expression of MRPL28. This would signal that the gene controls protein
biosynthesis (already known) that varies based on the type and amount of carbon
source present. This makes sense, because MRPL28 codes for a protein on the
mitochondrial large ribosomal subunit, meaning that MRPL28 might change
expression to alter the energy production in the cell. However, the
amounts of repression and induction are slight, and possibly insignificant
noise, meaning that MRPL28 is not one of the primary genes that affect the
energy production in the cell, but may have a lesser role in this pathway.
Evolution of expression
during glucose limitation (Stanford University).
Home | Search
| Figures | Data
| Info | Links
| SGD | Microarray_Homepage
| Name |
 |
|
Process |
|
Function |
| MRPL28 |
 |
|
protein biosynthesis |
|
structural protein of ribosome |
| YOR314W |
 |
|
biological_process unknown |
|
molecular_function unknown |
| ISY1 |
 |
|
mRNA splicing |
|
molecular_function unknown |
| SRP21 |
 |
|
not yet annotated |
|
not yet annotated |
| MMT1 |
 |
|
not yet annotated |
|
not yet annotated |
| YLR154C |
 |
|
biological_process unknown |
|
molecular_function unknown |
| YNL254C |
 |
|
biological_process unknown |
|
molecular_function unknown |
| PRI1 |
 |
|
DNA replication initiation* |
|
alpha DNA polymerase |
| HXT3 |
 |
|
transport |
|
not yet annotated |
| KAR3 |
 |
|
meiosis* |
|
motor |
| TFC4 |
 |
|
transcription initiation, from Pol III promoter |
|
RNA polymerase III transcription factor |
| YDR057W |
 |
|
biological_process unknown |
|
molecular_function unknown |
| YBL032W |
 |
|
biological_process unknown |
|
molecular_function unknown |
| YNL319W |
 |
|
biological_process unknown |
|
molecular_function unknown |
| YBL060W |
 |
|
biological_process unknown |
|
molecular_function unknown |
| FUN26 |
 |
|
biological_process unknown |
|
molecular_function unknown |
| YKR035C |
 |
|
biological_process unknown |
|
molecular_function unknown |
| SAS4 |
 |
|
not yet annotated |
|
molecular_function unknown |
| YJL218W |
 |
|
biological_process unknown |
|
molecular_function unknown |
| YEL018W |
 |
|
biological_process unknown |
|
molecular_function unknown |
| YFR035C |
 |
|
biological_process unknown |
|
molecular_function unknown |
Search
another gene name | Go to list of Databases Searched
| SGD Home
MRPL28 again shows very little response, which is not surprising
based on the results of the diauxic shift microarray. The limitation of glucose
for the yeast does not significantly affect the level of MRPL28 expression. The fact that
MRPL28 expression was not significantly affected signifies again that it is not involved in
the primary control of energy production for the cell. The data support the
already known fact that the protein encoded by MRPL28 is most likely a
structural protein in the large ribosomal subunit of the mitochondria, static in
its expression.
Expression in
response to environmental changes (Stanford University).
|
|
Expression in response to environmental changes for
MRPL28/YDR462W
|
|
Scale : (fold repression/induction)
Click on a color strip to see data for that gene.
Up to 20 similar genes are shown, with a Pearson correlation of > 0.8 to
the query gene
| Orf |
|
Gene |
|
 |
|
Process |
|
Function |
|
Component |
| YDR462W |
|
MRPL28 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial large ribosomal subunit |
| YBR269C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YBR273C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
* : indicates that more than one annotation exists for the gene.
Expression in response to environmental changes for MRPL28/YDR462W
Visit the Website |
|
SGDtm pages Database Copyright © 1997-2001 The Board
of Trustees of Leland Stanford Junior University. Permission to use the
information contained in this database was given by the researchers/institutes
who contributed or published the information. Users of the database are solely
responsible for compliance with any copyright restrictions, including those
applying to the author abstracts. Documents from this server are provided
"AS-IS" without any warranty, expressed or implied.
Search
another gene name | Go to list of Databases Searched
| SGD Home
MRPL28 shows some interesting trends in this microarray. First,
the heat shock spots show that MRPL28 is induced for all values except one
(which did not return any data, in gray). This could mean that the heat is
denaturing the structural proteins, so more must be created. Thus, MRPL28
is upregulated. This is consistent with the other stresses involving the heating
of the cell - MRPL28 is upregulated when ambient temperature is high enough to
denature structural proteins, then downregulated when that stress is removed.
The 37C to 25C shock shows downregulation of MRPL28 (which would be expected
with the "cooling" of the cell), and the shock of the different
starting temperatures to 37C shows upregulation of the gene (again consistent
with the "heating" of the cell). Other variations in expression
are consistent with the known role of MRPL28, the synthesis of proteins.
Expression during the
cell cycle (Stanford University, Cold Spring Harbor).
Home | Search
| Figures | Data
| Info | Links
| SGD | Microarray_Homepage
| Name |
Score |
Peak |
 |
| MRPL28 |
0.182 |
|
 |
| Reference Genes |
| CLN2 |
10.9 |
G1 |
 |
| HTA1 |
10.68 |
S |
 |
| CLB4 |
3.08 |
G2 |
 |
| SWI5 |
6.726 |
M |
 |
| ASH1 |
11.8 |
M/G1 |
 |
| Plot of MRPL28 (YDR462W)
|  |
Note: We only generated a list of genes with similar patterns of
expression for genes whose "aggregate CDC scores" were in the top 1200
(Score >= 0.95). MRPL28 did not have a score high enough for us to
generate such a list.
Search
another gene name | Go to list of Databases Searched
| SGD Home
MRPL28 produced spots that showed no significant change throughout the cell cycle.
In fact, a whole section of the microarray returned no data (shown in gray) for
MRPL28. One of the only conclusions that can be reached from this array is the fact that
the proteins that MRPL28 synthesizes do not vary with the stage in the cell cycle. It is also noted that
MRPL28 did not cluster with any genes which showed an aggregate CDC score >.95. Therefore, little can be drawn from this array set.
Expression
in response to histone depletion (The Whitehead Institute).
|
|
Expression in response to histone depletion for
MRPL28/YDR462W
|
|
Scale : (fold repression/induction)
Click on a color strip to see data for that gene.
Up to 20 similar genes are shown, with a Pearson correlation of > 0.8 to
the query gene
| Orf |
|
Gene |
|
 |
|
Process |
|
Function |
|
Component |
| YDR462W |
|
MRPL28 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial large ribosomal subunit |
| YMR188C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YGR138C |
|
TPO2 |
|
 |
|
biological_process unknown* |
|
spermine transporter* |
|
vacuolar membrane |
| YBR244W |
|
GPX2 |
|
 |
|
not yet annotated |
|
glutathione peroxidase |
|
not yet annotated |
| YER037W |
|
PHM8 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
cellular_component unknown |
| YFL026W |
|
STE2 |
|
 |
|
pheromone response (sensu Saccharomyces) |
|
mating-type alpha-factor pheromone receptor |
|
integral plasma membrane protein |
| YHL026C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YGR189C |
|
CRH1 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
cell wall |
| YDL173W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YKR018C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YDL048C |
|
STP4 |
|
 |
|
tRNA splicing |
|
molecular_function unknown |
|
not yet annotated |
| YGR007W |
|
MUQ1 |
|
 |
|
not yet annotated |
|
cholinephosphate cytidylyltransferase |
|
not yet annotated |
| YKL119C |
|
VPH2 |
|
 |
|
vacuolar acidification* |
|
molecular_function unknown |
|
endoplasmic reticulum membrane |
| YPR104C |
|
FHL1 |
|
 |
|
rRNA processing* |
|
transcription factor |
|
nucleus |
| YGR195W |
|
SKI6 |
|
 |
|
35S primary transcript processing* |
|
3'-5' exoribonuclease |
|
nuclear exosome (RNase complex)* |
| YGR031W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YFL039C |
|
ACT1 |
|
 |
|
establishment of cell polarity (sensu
Saccharomyces)* |
|
structural protein of cytoskeleton |
|
actin cortical patch (sensu Saccharomyces)* |
| YHR136C |
|
SPL2 |
|
 |
|
cell cycle |
|
protein kinase inhibitor |
|
not yet annotated |
| YBR016W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YGL097W |
|
SRM1 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YKL211C |
|
TRP3 |
|
 |
|
not yet annotated |
|
indole-3-glycerol-phosphate synthase |
|
not yet annotated |
* : indicates that more than one annotation exists for the gene.
SGDtm pages Database Copyright © 1997-2001 The Board
of Trustees of Leland Stanford Junior University. Permission to use the
information contained in this database was given by the researchers/institutes
who contributed or published the information. Users of the database are solely
responsible for compliance with any copyright restrictions, including those
applying to the author abstracts. Documents from this server are provided
"AS-IS" without any warranty, expressed or implied.
Search
another gene name | Go to list of Databases Searched
| SGD Home
MRPL28 shows little data again for the histone depletion
microarray. One would expect genes to show some change in expression if involved in any transcription
with this array, because changes in the histone concentrations would affect the yeast's ability to continue cellular pathways involving DNA replication or DNA-RNA
transcription. However, the translation of RNA into proteins seems
unaffected by histone depletion, and MRPL28 did not show any significant change
in expression.
Expression
during sporulation (UCSF, Stanford University).
|
|
Expression during sporulation for MRPL28/YDR462W
|
|
Scale : (fold repression/induction)
Click on a color strip to see data for that gene.
Up to 20 similar genes are shown, with a Pearson correlation of > 0.8 to
the query gene
| Orf |
|
Gene |
|
 |
|
Process |
|
Function |
|
Component |
| YDR462W |
|
MRPL28 |
|
 |
|
protein biosynthesis |
|
structural protein of ribosome |
|
mitochondrial large ribosomal subunit |
| YPL096W |
|
PNG1 |
|
 |
|
degradation of misfolded or incompletely
synthesized proteins* |
|
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
amidase |
|
nucleus* |
| YPL213W |
|
LEA1 |
|
 |
|
mRNA splicing |
|
not yet annotated |
|
not yet annotated |
| YHR091C |
|
MSR1 |
|
 |
|
not yet annotated |
|
arginine--tRNA ligase |
|
not yet annotated |
| YMR027W |
|
HRT2 |
|
 |
|
not yet annotated |
|
molecular_function unknown |
|
not yet annotated |
| YOL086C |
|
ADH1 |
|
 |
|
not yet annotated |
|
acylglycerone-phosphate reductase |
|
not yet annotated |
| YOL019W |
|
TOS7 |
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YDR353W |
|
TRR1 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YJR103W |
|
URA8 |
|
 |
|
not yet annotated |
|
CTP synthase |
|
not yet annotated |
| YCR030C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YCL051W |
|
LRE1 |
|
 |
|
not yet annotated |
|
molecular_function unknown |
|
not yet annotated |
| YGR124W |
|
ASN2 |
|
 |
|
not yet annotated |
|
asparagine synthase (glutamine-hydrolyzing) |
|
not yet annotated |
| YLR121C |
|
YPS3 |
|
 |
|
protein metabolism and modification |
|
aspartic-type endopeptidase |
|
plasma membrane |
| YHL021C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YJR125C |
|
ENT3 |
|
 |
|
actin filament organization* |
|
cytoskeletal adaptor |
|
actin cortical patch (sensu Saccharomyces) |
| YPR131C |
|
NAT3 |
|
 |
|
not yet annotated |
|
not yet annotated |
|
not yet annotated |
| YLR420W |
|
URA4 |
|
 |
|
not yet annotated |
|
dihydroorotase |
|
not yet annotated |
| YLR298C |
|
YHC1 |
|
 |
|
mRNA splicing |
|
not yet annotated |
|
not yet annotated |
| YPL168W |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YNL168C |
|
|
|
 |
|
biological_process unknown |
|
molecular_function unknown |
|
not yet annotated |
| YHL019C |
|
APM2 |
|
 |
|
endocytosis |
|
not yet annotated |
|
not yet annotated |
* : indicates that more than one annotation exists for the gene.
SGDtm pages Database Copyright © 1997-2001 The Board
of Trustees of Leland Stanford Junior University. Permission to use the
information contained in this database was given by the researchers/institutes
who contributed or published the information. Users of the database are solely
responsible for compliance with any copyright restrictions, including those
applying to the author abstracts. Documents from this server are provided
"AS-IS" without any warranty, expressed or implied.
Search
another gene name | Go to list of Databases Searched
| SGD Home
MRPL28 shows a repression for nearly every time point (excluding
0.5 hours) in the sporulation microarray. Initially, the repression is small, then becomes more pronounced, and
levels out after time=5.0 hours. Overall, the repression is less pronounced than in many other genes, which show a fold repression of >2.8. The peak for
MRPL28 repression is slightly >0.5 at time=5.0 hours. MRPL28 clusters with many genes of more significant repression, but many of them have functions having to do with protein synthesis. This is consistent with the arrays above
and for expected results. It seems that the cell is downregulating the
protein synthesis of MRPL28 during sporulation to allocate more energy to the
vital sporulation process.
CONCLUSIONS
Though the data that was received through these microarrays was limited, it
agrees with previous knowledge of MRPL28. MRPL28 is consistent in the fact that it shows little response to nearly all environmental stresses and cell cycle stage changes. Therefore
it can be assumed that MRPL28 synthesizes proteins that are not vital to the
cell cycle. The cell even downregulates MRPL28 during one specific process
(sporulation), possibly to allow the cell to "concentrate" on more
important pathways. However, when the cell is introduced to heat shock,
MRPL28 is upregulated, most likely to replace proteins lost to denaturation.
All of this data supports the belief that MRPL28 is in fact a structural protein
in the large ribosomal subunit in the mitochondria.
Links
Peter Lowry's Home Page
Davidson College
Davidson Biology Department
Davidson Genomics Homepage
This page was created by:
Peter Lowry
pelowry@davidson.edu
Davidson College
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