LOCUS YSPL972A 1258 bp DNA PLN 30-APR-1997 DEFINITION Yeast cps8 gene for actin, complete cds.

ACCESSION D84318 NID g1304268 KEYWORDS cps8; actin. SOURCE Schizosaccharomyces pombe (strain:L972) mating type h- DNA.

ORGANISM Schizosaccharomyces pombe Eukaryotae; mitochondrial eukaryotes; Fungi; Ascomycota; Archaeascomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces. REFERENCE 1 (bases 1 to 1258)

AUTHORS Ishiguro,J. and Kobayashi,W. TITLE Direct Submission JOURNAL Submitted (05-APR-1996) to the DDBJ/EMBL/GenBank databases. Junpei Ishiguro, Konan University, Department of Biology, Faculty of Science; Higashinada-ku, Okamoto 8-9-1, Kobe, Hyogo 658, Japan (E-mail:ishiguro@base2.ipc.konan-u.ac.jp, Tel:078-435-2516, Fax:078-435-2539) REFERENCE 2 (sites)

AUTHORS Ishiguro,J. and Uhara,Y. TITLE Isolation and characterization of mutants supersensitive to the spindle poison, isopropyl N-3-chlorophenyl carbamate (CIPC) in the fission yeast Schizosaccharomyces pombe JOURNAL Jpn. J. Genet. 67 (2), 97-109 (1992) MEDLINE 92398982 REFERENCE 3 (sites)

AUTHORS Ishiguro,J. and Yamada,N. TITLE A novel mutation involved in the mitotic checkpoint in the fission yeast Schizosaccharomyces pombe JOURNAL Jpn. J. Genet. 68 (4), 265-276 (1993) MEDLINE 94121949 REFERENCE 4 (sites)

AUTHORS Ishiguro,J. and Kobayashi,W. TITLE An actin point-mutation neighboring the 'hydrophobic plug' causes defects in the maintenance of cell polarity and septum organization in the fission yeast Schizosaccharomyces pombe JOURNAL FEBS Lett. 392 (3), 237-241 (1996) MEDLINE 96371017 FEATURES Location/Qualifiers source 1..1258 /organism="Schizosaccharomyces pombe" /strain="L972" /db_xref="taxon:4896" /sex="h-" gene 73..1200 /gene="cps8" CDS 73..1200 /gene="cps8" /codon_start=1 /product="actin" /db_xref="PID:d1012991" /db_xref="PID:g1304269"

/translation="MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIM VGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP CLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVT HTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCY VALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENADIHE ATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPE RKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF" BASE COUNT 302 a 286 c 270 g 400 t

ORIGIN 1 gccgtgttat aacttaccgt ttaccaacta cattttttgt aacgaaccaa aaaaccctca

61 aaagacaaga ccatggaaga agaaatcgca gcgttggtta ttgataatgg ctctggtatg

121 tgcaaagccg gtttcgctgg agatgatgcc cctagagctg tattcccctc gattgtcggt

181 agaccccgtc accatggtat tatggtaggt atgggacaaa aggattccta cgttggtgat

241 gaagctcaaa gcaagcgtgg tattttgacc ttgaagtacc ccattgagca cggtattgtc

301 aacaactggg atgatatgga aaagatttgg catcacactt tctacaacga gcttcgtgtt

361 gctcctgagg agcacccttg cttgttgact gaggctcctt tgaaccccaa atccaaccgt

421 gagaagatga ctcaaattat tttcgaaacc tttaatgcac ctgcctttta tgttgctatt

481 caagctgttt tgtctttgta tgcctctggt cgtaccactg gtatcgtctt ggactctggt

541 gatggtgtta cccacactgt tcccatttat gagggttatg ctcttcctca tgctatcatg

601 cgtcttgatc tcgccggtcg tgacttgact gactacctta tgaagattct catggagcgt

661 ggttatactt tctctactac cgccgaacgt gaaattgttc gtgacatcaa ggagaaactt

721 tgctacgtcg ctttggactt tgagcaagaa cttcaaactg ctgctcaatc ttcctccctt

781 gagaaatctt atgagcttcc tgatggtcaa gttatcacta tcggaaacga gcgtttccgt

841 gcccctgaag ctcttttcca accctcagct ttgggtcttg aaaacgccga tattcatgag

901 gctacttaca actctatcat gaaatgtgat gttgatattc gtaaagattt gtacggtaac

961 gttgttatgt ctggtggtac cactatgtat cccggtattg ccgatcgtat gcaaaaggag

1021 attcaagccc ttgctcctag ctccatgaag gtcaagattg ttgctcctcc tgagcgtaaa

1081 tactctgtct ggattggtgg atccattctt gcttctcttt ctactttcca acagatgtgg

1141 atctccaagc aagagtatga tgagagcgga cctggtatcg tttaccgtaa gtgcttctaa

1201 gctcctctta ctttttgtaa cgttttttac atacttttga ataacatcga ttcttctg //