LOCUS 123891 918 aa 01-OCT-1996

DEFINITION HEXOKINASE, TYPE I (HK I) (BRAIN FORM HEXOKINASE).

ACCESSION 123891 PID g123891 DBSOURCE SWISS-PROT: locus HXK1_BOVIN, accession P27595 class: standard. created: Aug 1, 1992. sequence updated: Aug 1, 1992. annotation updated: Oct 1, 1996. xrefs: gi: 163151, gi: 163152 xrefs (non-sequence databases): HSSP P35557, PROSITE PS00378

KEYWORDS TRANSFERASE; KINASE; GLYCOLYSIS; ALLOSTERIC ENZYME; DUPLICATION; ATP-BINDING; MEMBRANE.

SOURCE Bos taurus.

ORGANISM Bos taurus Eukaryotae; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria; Artiodactyla; Ruminantia; Pecora; Bovoidea; Bovidae; Bovinae; Bos.

REFERENCE 1 (residues 1 to 918)

AUTHORS Griffin,L.D., MacGregor,G.R., Muzny,D.M., Harter,J., Cook,R.G. and McCabe,E.R. TITLE Synthesis and characterization of a bovine hexokinase 1 cDNA probe by mixed oligonucleotide primed amplification of cDNA using high complexity primer mixtures

JOURNAL Biochem. Med. Metab. Biol. 41 (2), 125-131 (1989)

MEDLINE 89247146 REMARK SEQUENCE FROM N.A.

REFERENCE 2 (residues 1 to 918)

AUTHORS Griffin,L.D., Gelb,B.D., Wheeler,D.A., Davison,D., Adams,V. and McCabe,E.R.

TITLE Mammalian hexokinase 1: evolutionary conservation and structure to function analysis

JOURNAL Genomics 11 (4), 1014-1024 (1991)

MEDLINE 92147096

REMARK SEQUENCE FROM N.A.

COMMENT [CATALYTIC ACTIVITY] ATP + D-HEXOSE = ADP + D-HEXOSE 6-PHOSPHATE. [ENZYME REGULATION] HEXOKINASE IS AN ALLOSTERIC ENZYME INHIBITED BY ITS PRODUCT GLC-6-P. [PATHWAY] FIRST STEP OF SEVERAL METABOLIC PATHWAYS. [SUBUNIT] MONOMER. [SUBCELLULAR LOCATION] BOUND TO THE OUTER MITOCHONDRIAL MEMBRANE. ITS HYDROPHOBIC N-TERMINAL SEQUENCE MAY BE INVOLVED IN MEMBRANE BINDING. IN VERTEBRATES THERE ARE FOUR MAJOR GLUCOSE-PHOSPHORYLATING ISOENZYMES, DESIGNATED HEXOKINASE I, II, III AND IV (GLUCOKINASE). [SIMILARITY] THE N- AND C-TERMINAL HALVES OF THIS HEXOKINASE SHOW EXTENSIVE SEQUENCE SIMILARITY TO EACH OTHER. THE CATALYTIC ACTIVITY IS ASSOCIATED WITH THE C-TERMINUS WHILE REGULATORY FUNCTION IS ASSOCIATED WITH THE N-TERMINUS. [SIMILARITY] STRONG, TO OTHER HEXOKINASES.

FEATURES Location/Qualifiers source 1..918 /organism="Bos taurus" /db_xref="taxon:9913" 1..918 Region 1..12 /note="HYDROPHOBIC." /region_name="Domain" Protein 1..918 /product="HEXOKINASE, TYPE I" /EC_number="2.7.1.1" Region 13..475 /note="REGULATORY." /region_name="Domain" Site 84..89 /note="ATP." /site_type="np-binding" Region 149..175 /note="GLUCOSE-BINDING." /region_name="Domain" Region 476..918 /note="CATALYTIC." /region_name="Domain" Site 532..537 /note="ATP." /site_type="np-binding" Site 558 /note="ATP." /site_type="binding" Region 597..623 /note="GLUCOSE-BINDING." /region_name="Domain"

ORIGIN 1 miaaqllayy ftelkddqvk kidkylyamr lsdetlldim nrfkkemkng lsrdfnptat 61 vkmlptfvrs ipdgsekgdf ialdlggssf rilrvqvnhe qnrpvhmese vydtpenimh 121 gsgsqlfdhv leclgdfmek kkikdkklpv gftfsfpcrq skidqailit wtkrfkarga 181 egnyvvklld kaikkrgdyd anivavvndt vgtmidcgyd dqhcevglii gtgtnacyme 241 elrqidfgwg ddgrmcinte wgdlgddgsl edirkefdre frrgslnpgk qrfekmvsgr 301 ymedvvrlvl vkmakegllf egritpellt rgkfntsdvs aiekdkeglh nakeiltrlg 361 versdddcvs vqhvctivsf rsanlvaatl gailnrlrdn kstprlrttv rvdgslykth 421 pqysrrfhkt lrrlvpdsdv rfllsesgtg kgaamvtava yrlaeqhrqi eetlahfrls 481 kqtlmevkkr lrtememglr ketnsnatvn mlpsflrsip dgtedgdfla ldlggtnfrv 541 llvkirsgkk stvemhnkiy ripieimqgt geelfdhivs cisdfldymg ikgprmplgf 601 tfsfpcqqts ldagilitwt kgfkatdcvg hdvvtllrda vkrreefdld vvavvndtvg 661 tmmtcayeep tcevglivgt gsnacymeem knvemvegnq rqmcinmewg afgdngcsdd 721 irtdfdkvvd eyslnsgnqr fenmisgiyl geivrnilid ftkkgflfrg qiseplktrg 781 ifetkflsqi esdrlallqv railqqlgln stcddsilvk tvcgvvskra aqlcgagmaa 841 vvekirenrg ldrlnvtvgv dgtlyklhpq fsrimhqtvk elspkcnvsf llsedgsgkg 901 aalitavgvr lrgesais