LOCUS MUSHEXOKB 3071 bp mRNA ROD 16-MAY-1997

DEFINITION Mus musculus hexokinase (Hk-1) mRNA, complete cds.

ACCESSION L16949 NID g309292

KEYWORDS hexokinase.

SOURCE house mouse.

ORGANISM Mus musculus Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata; Vertebrata; Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.

REFERENCE 1 (bases 1 to 3071)

AUTHORS Mori,C., Welch,J.E., Fulcher,K.D., O'Brien,D.A. and Eddy,E.M.

TITLE Unique hexokinase messenger ribonucleic acids lacking the porin-binding domain are developmentally expressed in mouse spermatogenic cells

JOURNAL Biol. Reprod. 49 (2), 191-203 (1993)

MEDLINE 93385326

COMMENT *Reference [1] reports bases 1 through 286; updated sequence to concur with publication, NCBI: 25-FEB-1997.

FEATURES Location/Qualifiers source 1..3071 /organism="Mus musculus" /strain="CD-1" /db_xref="taxon:10090" /clone="Hk1-sb" /cell_type="germ cell" /dev_stage="gamete" /tissue_type="testis" mRNA 1..3071 /gene="Hk-1" /evidence=experimental 5'UTR <1..187 /gene="Hk-1" gene 1..3071 /gene="Hk-1" misc_feature 1..117 /gene="Hk-1" /note="unique region" /evidence=experimental misc_feature 118..257 /gene="Hk-1" /note="region shared with L16948 and L16950" /evidence=experimental CDS 188..3025 /gene="Hk-1" /EC_number="2.7.1.1" /codon_start=1 /evidence=experimental /product="hexokinase" /db_xref="PID:g1850140" /

translation="MGQNCQRGQAVDVEPKIRPPLTEEKIDKYLYAMRLSDEILIDIL TRFKKEMKNGLSRDYNPTASVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVN HEKSQNVSMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSF PCRQSKIDEAVLITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTM MTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLED IRTEFDRELDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKESLLFEGRITPELLT RGKFTTSDVAAIETGWELSPDRRWYQAYMRCTQDTHRDKEGVQNAKEILTRLGVEPSH DDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKMHPQY SRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLSHFRLSK QALMEVKKKLRSEMEMGLRKETNSRATVKMLPSYVRSIPDGTEHGDFLALDLGGTNFR VLLVKIRSGKKRTVEMHNKIYSIPLEIMQGTGDELFDHIVSCISDFLDYMGIKGPRMP LGFTFSFPCKQTSLDCGILITWTKGFKATDCVGHDVATLLRDAVKRREEFDLDVVAVV NDTVGTMMTCAYEEPSCEIGLIVGTGSNACYMEEMKNVEMVEGNQGQMCINMEWGAFG DNGCLDDIRTDFDKVVDEYSLNSGKQRFEKMISGMYLGEIVRNILIDFTKKGFLFRGQ ISEPLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKR AAQLCGAGMAAVVQKIRENRGLDHLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCT VSFLLSEDGSGKGAALITAVGVRLRGDPTNA"

misc_feature 258..1232 /gene="Hk-1" /note="Hk1 sequence" /evidence=experimental misc_feature 1233..1301 /gene="Hk-1" /note="unique region" /evidence=experimental misc_feature 1302..3071 /gene="Hk-1" /note="Hk1 sequence" /evidence=experimental 3'UTR 3026..3071 /gene="Hk-1" /evidence=experimental

BASE COUNT 783 a 789 c 890 g 609 t

ORIGIN 1 ccttaacacc tgggaccatg gagggccccc ctcttctcaa ggcttgctag gttagaccag 61 taactacatc aaggactcca acccaccagc tagagaccgt tcctcagaca cctgagaagg 121 atcagaaaac cccgcttctg aggacagaag acctctggaa aaacagtgta gccatcacct 181 ctacactatg ggacagaact gccagcgagg acaggctgta gatgttgaac caaaaatacg 241 accccctctc acagaggaaa agattgataa gtatctgtat gccatgcggc tctctgatga 301 aattctgata gatatcctga cacgcttcaa gaaagagatg aagaatggcc tctcccggga 361 ttataaccca acggcctccg tcaagatgct gccaaccttt gtccggtcca ttccggacgg 421 ctcagaaaag ggggatttca ttgcactgga tctcggcggg tcttcctttc gaatcctgcg 481 ggtgcaggtg aaccacgaga agagtcagaa cgtcagcatg gagtctgagg tctacgacac 541 cccagagaac atcgtgcacg gcagtggaag ccagcttttt gatcacgtcg ctgaatgcct 601 cggagacttc atggagaaaa ggaagatcaa ggacaagaaa ttacccgtgg gattcacgtt 661 ttccttcccg tgccgacaat ccaaaataga cgaggccgta ctgatcacgt ggacaaagcg 721 gttcaaagcc agtggcgtgg aaggggcgga tgtggtcaag ctgctgaata aagccattaa 781 gaagcgaggg gactatgacg ctaacattgt agctgtggtg aatgacacag tggggaccat 841 gatgacctgc ggctacgatg accaacagtg tgaagtcggc ctgatcattg gcactggcac 901 caatgcttgc tacatggagg aactgcgaca catcgacctg gtggaaggcg atgaggggag 961 gatgtgtatt aacacggaat ggggagcctt tggggatgat gggtccctgg aagacattcg 1021 aacagagttt gacagagagt tagaccgggg atccctcaac cctgggaaac agctgttcga 1081 gaagatggtg agcggcatgt acatggggga gctggtccgg ctgatcctgg tgaagatggc 1141 caaggaaagc ctcttatttg aagggcgcat tactccagag ctgctcacga ggggcaagtt 1201 caccactagc gacgtagccg ccattgaaac gggatgggaa ctctcccctg accgcaggtg 1261 gtaccaggca tatatgaggt gcacacaaga cacccacagg gataaggaag gcgttcaaaa 1321 tgccaaggaa atcttgaccc gcctgggagt ggagccgtct cacgatgact gcgtatcggt 1381 ccagcacgta tgcacgatcg tctccttccg atcagccaac ctggtggctg ccacgctcgg 1441 tgccatcttg aaccgcctgc gggacaataa gggcacgccc aggctgcgga ccacagttgg 1501 cgtagacggt tctctctaca agatgcaccc acagtattcc cggcggttcc acaagaccct 1561 gaggcgcctg gtgcctgact cggacgtccg gttcctcctc tcggagagtg gcagtggcaa 1621 gggagccgcc atggtgaccg ctgtggccta ccgcctggcc gagcagcacc ggcagattga 1681 ggaaaccctg tcccacttcc gcctcagcaa gcaggcactg atggaggtga agaagaagct 1741 gcggtcagag atggaaatgg ggctgagaaa ggagaccaac agcagagcta cggtcaaaat 1801 gctgccttct tatgtgcgga gcatcccaga tgggactgag catggtgact tcctggcctt 1861 ggatctcgga ggaacgaatt tccgagtcct actggtaaag atccgtagtg ggaaaaagag 1921 aacagtggag atgcacaaca agatctactc cattcccctg gaaatcatgc agggcaccgg 1981 ggatgagctg tttgatcaca ttgtctcctg catctccgac ttcctggact acatggggat 2041 caaaggcccc cggatgcctc tgggcttcac cttctcgttt ccctgcaagc agacgagcct 2101 agattgcgga atcttgatca cgtggacaaa gggattcaaa gccaccgact gtgtgggtca 2161 cgatgtagcc actttactga gggatgctgt aaaaaggaga gaggaatttg acctggatgt 2221 ggtggctgtg gtcaacgaca ccgtgggcac catgatgact tgtgcttatg aagaaccttc 2281 ttgtgagatt ggactcatcg tggggactgg cagcaatgcc tgctacatgg aggagatgaa 2341 aaacgtggag atggtggagg ggaaccaggg ccagatgtgc atcaatatgg aatggggtgc 2401 ctttggtgac aacggctgtc tggacgacat cagaacagac ttcgacaaag tggtggacga 2461 atattctcta aacagtggga aacaaaggtt tgagaagatg atcagtggaa tgtacctggg 2521 tgagatcgtc cgtaacatcc tgattgactt caccaagaaa ggcttcctct tccggggaca 2581 gatctctgag ccactcaaga cccgaggcat cttcgagacc aagtttctct ctcagatcga 2641 gagtgaccga ttagcgctgc tccaggtgcg ggccatcctt cagcagctgg gtctaaacag 2701 cacgtgcgac gacagtatcc tggtcaagac cgtgtgtggg gtggtgtcca agcgggcggc 2761 ccagctgtgt ggtgccggca tggccgccgt ggtgcaaaag atccgagaga acagaggcct 2821 agaccacctg aatgtaaccg tgggcgtgga cgggacgctc tacaaactcc atccacactt 2881 ctccagaatc atgcaccaaa cagtgaagga actgtcacca aagtgtaccg tgtccttcct 2941 cctgtctgaa gacggcagcg gcaagggggc cgcccttatc acagctgtgg gcgtgcggct 3001 cagaggagac cctacgaacg cctaagagca ggatcctccc agcccccagc ccgccaccct 3061 tccagcactc c