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PCR Primers for 5 Yeast (Saccharomyces cerevisiae) IDH Genes

IDH1 Forward: 5' ATGCTTAACAGAACAATTGC 3' Tm = 46° C
IDH1 Reverse: 5' TTACATGGTAGATAATTTGTTG 3' Tm = 46° C

IDH2 Forward: 5' ATGTTGAGAAATACTTTTTTTTAG 3' Tm = 45° C
IDH2 Reverse: 5' TTATAATCTCTTGATGACTG 3' Tm = 44° C

IDP1 Forward: 5' ATGAGTATGTTATCTAGAAG 3' Tm = 44° C
IDP1 Reverse: 5' TTACTCGATCGACTTGATTT 3' Tm = 46° C

IDP2 Forward: 5' ATGACAAAGATTAAGGTAGC 3' Tm = 46° C
IDP2 Reverse: 5' TTACAATGCAGCTGCCTCGA 3' Tm = 52° C

IDP3 Forward: 5' ATGAGTAAAATTAAAGTTGTTCAT 3' Tm = 45° C
IDP3 Reverse: 5' TTATAGTTTGCACATACCTT 3' Tm = 44° C

© Copyright 2002 Department of Biology, Davidson College, Davidson, NC 28035
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