This
web page was produced as an assignment for an undergraduate course at Davidson College
Molecular Biology(Bio
304)
Ed
Haas
In the laboratory portion of this course, we are cloning the five
Saccharomyces cerevisiae(bakers yeast) genes: IDH1, IDH2, IDP1, IDP2, and
IDP3(accession numbers: YLN037C, YOR137W, YDL066W, YLR174W, and YNL009W
respectively). So far we have
cloned them through PCR, ligated them into the pQE-30
UA vector, and inserted this plasmid into the JM109 strain of e.coli.
Go here
to see the list of primers we used for PCR.
These genes are all involved in cellular respiration. To learn more about this click here.
Links to:
The gene sequence of: IDH1,
IDH2,
IDP1,
IDP2,
and IDP3.
The amino acid sequence of: IDH1,
IDH2,
IDP1,
IDP2,
and IDP3.
Six cutter restriction map for: IDH1,
IDH2,
IDP1,
IDP2,
and IDP3.
See SDS-PAGE results of our PCR products here. My lab partner Elizabeth Sellers and I
were cloning IDP3 in the Thursday lab.
Figure 1. This
electron micrograph of strain DY150 Saccharomyces cerevisiae was taken by
Diane Nowicki and Ryan Liermann.
This image was taken from a public SGD
site stating permission for public use.
Send comments, questions, and
suggestions to: edhaas@davidson.edu