This web page was created for an undergraduate molecular biology course at Davidson College
All Melting Temps are between 44-46 C
IDH1:
Forward: 5' ATGCTTAACAGAACAATTGC 3'
Reverse: 5' TTACATGGTAGATAATTTGTTG 3'
IDH2:
Forward: 5' ATGTTGAGAAATACTTTTTTTAG 3'
Reverse: 5' TTATAATCTCCTTCAGTGAA 3'
IDP1:
Forward: 5' ATGAGTATGTTATCTAGAAG 3'
Reverse: 5' TTACTCGATCGACTTGATTT 3'
IDP2:
Forward: 5' ATGACAAAGATTAAGGTAGC 3'
Reverse: 5' TTACAATGCAGCTGCCT 3'
IDP3:
Forward: 5' ATGAGTAAAATTAAAGTTGTTC 3'
Reverse: 5' TTATAGTTTACACTGTCTTC 3'
Check The Actual Primers Used by Class
Check Out Our Results (Chris Norbet and I are cloning IDH1 on Thursday afternoon)
Contact Kevin James with comments or questions.