Map of Six-cutter Restriction Sites for IDH1:
This page was produced as part of as undergraduate course at Davidson College.
Molecular Descriptions of IDH genes in Saccharomyces cerevisiae
Or Experiment to Check for Orientation of Gene Insert in Plasmid
IDH1
Accession Number in NCBI: M95203
Associated Coenzyme: NAD+
PCR Primer:Forward: 5' ATGCTTAACAGAACAATTGC 3'
Reverse: 5' TTACATGGTAGATAATTTGTTG 3'
His6 in-frame sequence with first 10 Amino Acids of IDH1: RGS-HHHHHH-SHVISSIAS-MLNRTIAKRT
Map of Six-cutter Restriction Sites for IDH1:
IDH2
Accession Number in NCBI: M74131
Associated Coenzyme: NAD+
PCR Primer Forward: 5' ATGTTGAGAAATACTTTTTTTAG 3'
Reverse: 5' TTATAATCTCCTTCAGTGAA 3'
His6 in-frame sequence with first 10 Amino Acids of IDH2: RGS-HHHHHH-SHVISSIAS-MLRNTFFRNT
Map of Six-Cutter Restriction Sites for IDH2:
IDP1
Accession Number in NCBI: M57229
Associated Coenzyme: NADP+
PCR Primer Forward: 5' ATGAGTATGTTATCTAGAAG 3'
Reverse: 5' TTACTCGATCGACTTGATTT 3'
His6 in-frame sequence with first 10 Amino Acids of IDP1: RGS-HHHHHH-SHVISSIAS-MSMLSRRLFS
Map of Six-Cutter Restriction Sites for IDP1:
IDP2
Accession Number in NCBI: L26312
Associated Coenzyme: NADP+
PCR Primer Forward: 5' ATGACAAAGATTAAGGTAGC 3'
Reverse: 5' TTACAATGCAGCTGCCT 3'
His6 in-frame sequence with first 10 Amino Acids of IDP2: RGS-HHHHHH-SHVISSIAS-MTKIKVANPI
Map of Six-Cutter Restriction Sites for IDP2:
IDP3
Acession Number in SGD (SGDID): S0004954
Associated Coenzyme: NADP+
PCR Primer Forward: 5' ATGAGTAAAATTAAAGTTGTTC 3'
Reverse: 5' TTATAGTTTACACTGTCTTC 3'
His6 in-frame sequence with first 10 Amino Acids of IDP3: RGS-HHHHHH-SHVISSIAS-MSKIKVVHPI
Map of Six-Cutter Restriction Sites for IDP3:
Contact: Peter Leese or view Molecular Biology at Davidson