This web page was produced by Zack Perfect as an assignment for an undergraduate course at Davidson College

      The Wonder Yeast Saccharomyces cerevisiae and its IDH genes

sacdisc.jpg (6751 bytes)          The yeast Saccharomyces cerevisiae has five IDH (isocitrate dehydrogenase) genes IDH1, IDH2, IDP1, IDP2, or IDP3. The goal of our lab is to clone all five IDH genes and express their proteins in bacteria. Each group was responsible for one of the genes; ours was IDH1. We used the Qiagen pQE-30 UA Plasmid for transformation. We designed appropriate primers, mapped out restriction digests, etc. based on the nature of our gene. Below is some general information on the genes we investigated in lab.

 

The Five Genes of Interest

IDH1button.jpg (9996 bytes)IDH2button.jpg (9894 bytes)IDP1button.jpg (9573 bytes)

IDP2button.jpg (10025 bytes)IDP3button.jpg (10180 bytes)

 

If you are inspired to replicate the entire experiment on your own, or are just curious as to some of the specifics of the protocol, this is a helpful overview.

How to determine whether the IDH1 gene inserted in the correct orientation.

 

 

References

Campbell, M.A. 2002. Lecture and Lab Handouts. Davidson College. http://www.bio.davison.edu/molecular

Saccharomyces Genomic Database  http://genome-www.stanford.edu/Saccharomyces/

 

Saccharomyces cerevisiae is nice, but come on, there's this yeast, and then there's everything else.

 

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