"This web page was produced as an assignment for an undergraduate course at Davidson College."
IDH1
Accession Number: Z71313 and GI:1301885
Gene Information:
IDH1 is located on chromosome XIV and encoded on the Crick strand. IDH1 plays a
part in glutamate biosynthesis, isocitrate metabolism, and the tricarboxylic
acid cycle. The gene product, isocitrate dehydrogenase 1 alpha-4-beta-4
subunit, is located in the mitchondrial matrix. (http://genome-www4.stanford.edu/cgi-bin/SGD/locus.pl?locus=IDH1)
Protein Information:
Protein Information: (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=protein&list_uids=1301886&dopt=GenPept)
Amino
Acid Sequence:
1 mlnrtiakrt lataaqaert lpkkyggrft vtlipgdgvg keitdsvrti feaenipidw
61 etinikqtdh kegvyeaves lkrnkiglkg lwhtpadqtg hgslnvalrk qldiyanval
121 fkslkgvktr ipdidlivir entegefsgl ehesvpgvve slkvmtrpkt eriarfafdf
181 akkynrksvt avhkanimkl gdglfrniit eigqkeypdi dvssiivdna smqavakphq
241 fdvlvtpsmy gtilgnigaa liggpglvag anfgrdyavf epgsrhvgld ikgqnvanpt
301 amilsstlml nhlglneyat riskavheti aegkhttrdi ggsssttdft neiinklstm
Genbank Open Reading Frame Finder (must enter accession number). This site shows the gene sequence as well as the amino acid sequence together.
6-Cutter Restriction Sites:
Fig. 1 This is a restriction map of six-cutter restriction enzymes for the IDH1 gene from the Saccharomyces Genome Database. (http://genome-www.stanford.edu/Saccharomyces/tips/tip6_remap.html, first erase the content in the box entitled Type or Paste DNA Sequence. Then one must enter gene name in box entitled Enter a Sequence Name and finally select in the Choose Restriction Enzymes box "six-base cutters")
PCR Primer:
IDH1
Forward: 5' ATGCTTAACAGAACAATTGC 3' Tm= 46° C
IDH1
Reverse: 5' TTACATGGTAGATAATTTGTTG 3' Tm= 46° C
In-Frame Fusion Protein of His6-IDH:
Histidine-Histidine-Histidine-Histidine-Histidine-Histidine-Methionine-Leucine-Asparagine-Arginine-Threonine-Isoleucine-Alanine-Lysine-Arginine-Threonine
Coenzyme:
NAD+
For questions or comments contact elsellars@davidson.edu