"This web page was produced as an assignment for an undergraduate course at Davidson College."
IDP1
Accession Number: Z74114 and GI:1431073
Gene Information:
This gene is found on chromosome IV. The gene product, NADP-dependent
isocitrate dehydrogenase, is located in the mitochondria. The gene product is
involved in glutamate biosynthesis and isocitrate metabolism. (http://genome-www4.stanford.edu/cgi-bin/SGD/locus.pl?locus=IDP1)
Protein Information:
Protein Information (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=protein&list_uids=1431074&dopt=GenPept)
Amino
Acid Sequence
1 msmlsrrlfs tsrlaafski kvkqpvveld gdemtriiwd kikkklilpy ldvdlkyydl
61 svesrdatsd kitqdaaeai kkygvgikca titpdearvk efnlhkmwks pngtirnilg
121 gtvfrepivi priprlvprw ekpiiigrha hgdqykatdt lipgpgslel vykpsdptta
181 qpqtlkvydy kgsgvamamy ntdesiegfa hssfklaidk klnlflstkn tilkkydgrf
241 kdifqevyea qykskfeqlg ihyehrlidd mvaqmikskg gfimalknyd gdvqsdivaq
301 gfgslglmts ilvtpdgktf eseaahgtvt rhyrkyqkge etstnsiasi fawsrgllkr
361 geldntpalc kfanilesat lntvqqdgim tkdlalacgn nersayvtte efldavekrl
421 qkeiksie
Genbank Open Reading Frame Finder (must enter accession number). This site shows the gene sequence as well as the amino acid sequence together.
6-Cutter Restriction Sites:
Fig. 3 This is a restriction map of six-cutter restriction enzymes for the IDP1 gene from the Saccharomyces Genome Database. (http://genome-www.stanford.edu/Saccharomyces/tips/tip6_remap.html, first erase the content in the box entitled Type or Paste DNA Sequence. Then one must enter gene name in box entitled Enter a Sequence Name and finally select in the Choose Restriction Enzymes box "six-base cutters")
PCR Primer:
IDP1
Forward: 5' ATGAGTATGTTATCTAGAAG 3' Tm= 44° C
IDP1
Reverse: 5' TTACTCGATCGACTTGATTT 3' Tm= 46° C
In-Frame Fusion Protein of His-6 IDP:
Histidine-Histidine-Histidine-Histidine-Histidine-Histidine-Methionine-Serine-Methionine-Leucine-Serine-Arginine-Arginine-Leucine-Phenylalanine-Serine
Coenzyme:
NADP+
For questions or comments contact elsellars@davidson.edu