"This web page was produced as an assignment for an undergraduate course at Davidson College."
IDP2:
Accession Number: L26312 and GI:457396
Gene Information:
This gene is found on chromosome XII. Much like all of the preceeding genes,
the gene product of IDP2 is involved in glutamate biosynthesis and isocitrate
metabolism. The gene product, NADP-dependent isocitrate dehydrogenase, is
located in the cytosol (http://genome-www4.stanford.edu/cgi-bin/SGD/locus.pl?locus=IDP2).
Protein Information:
Protein Information (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=protein&list_uids=736722&dopt=GenPept)
Amino
Acid Sequence
1 mtkikvanpi vemdgdeqtr iiwsfnqdkl vlpyldvdlk yydlsveyrd qtndqvtvds
61 atatlkyrva vkcatitpde arveefhlkk mwkspngtir nilggtvfre piiipriprl
121 vpqwekpiii grhafgdqyk atdvivpeeg elrlvyksks gthdvdlkvf dypehggvam
181 mmynntdsie gfakasfela ierklplyst tkntilkkyd gkfkdvfeam ylevikrsle
241 slgiwyehrl iddmvaqmlk skggyimamk nydgdvesdi vaqgfgslgl mtsvlitpdg
301 ktfesdrahg tvtrhltdyd kgretstnsi asifawtrgi iqrgkldntp dvvkfgqile
361 satvntvqed gimtkdlali lgksersayv tteefidave srlkkefeaa a
Genbank Open Reading Frame Finder (must enter accession number). This site shows the gene sequence as well as the amino acid sequence together.
6-Cutter Restriction Map:
Fig. 4. This is a restriction map of six-cutter restriction enzymes for the IDP2 gene from the Saccharomyces Genome Database. (http://genome-www.stanford.edu/Saccharomyces/tips/tip6_remap.html, first erase the content in the box entitled Type or Paste DNA Sequence. Then one must enter gene name in box entitled Enter a Sequence Name and finally select in the Choose Restriction Enzymes box "six-base cutters")
PCR Primer:
IDP2
Forward: 5' ATGACAAAGATTAAGGTAGC 3' Tm=46° C
IDP2
Reverse: 5' TTACAATGCAGCTGCCTCGA 3' Tm= 52° C
In-Frame Fusion Protein of His-6 IDP:
Histidine-Histidine-Histidine-Histidine-Histidine-Histidine-Methionine-Threonine-Lysine-Isoleucine-Lysine-Valine-Alanine-Asparagine-Proline-Isoleucine
Coenzyme:
NADP+
For questions or comments contact elsellars@davidson.edu