"This web page was produced as an assignment for an undergraduate course at Davidson College."
IDP3
Accession Number: GI:6324319 and SGD:S0004954
Gene Information:
This gene is found on chromosome XIV. IDP3 is involved in NADPH regeneration,
fatty acid beta-oxidation, and isocitrate metabolism. The gene product is
NADP-dependent isocitrate dehydrogenase. (http://genome-www4.stanford.edu/cgi-bin/SGD/locus.pl?locus=IDP3)
Protein Information:
Protein Information (http://www.ncbi.nlm.nih.gov/cgi-bin/Entrez/getfeat?gi=6323989&id=335&entity=19)
Amino
Acid Sequence
1 mskikvvhpi vemdgdeqtr viwklikekl ilpyldvdlk yydlsiqerd rtndqvtkds
61 syatlkygva vkcatitpde armkefnlke mwkspngtir nilggtvfre piiipkiprl
121 vphwekpiii grhafgdqyr atdikikkag klrlqfssdd gkenidlkvy efpksggiam
181 amfntndsik gfakasfela lkrklplfft tkntilknyd nqfkqifdnl fdkeykekfq
241 alkityehrl iddmvaqmlk skggfiiamk nydgdvqsdi vaqgfgslgl mtsilitpdg
301 ktfeseaahg tvtrhfrkhq rgeetstnsi asifawtrai iqrgkldntd dvikfgnlle
361 katldtvqvg gkmtkdlalm lgktnrssyv tteefideva krlqnmmlss nedkkgmckl
421
Genbank Open Reading Frame Finder (must enter accession number). This site shows the gene sequence as well as the amino acid sequence together.
6-Cutter Restricion Map:
Fig. 5 This is a restriction map of six-cutter restriction enzymes for the IDP3 gene from the Saccharomyces Genome Database. (http://genome-www.stanford.edu/Saccharomyces/tips/tip6_remap.html, first erase the content in the box entitled Type or Paste DNA Sequence. Then one must enter gene name in box entitled Enter a Sequence Name and finally select in the Choose Restriction Enzymes box "six-base cutters")
PCR Primer:
IDP3
Forward: 5' ATGAGTAAAATTAAAGTTGTTCAT 3' Tm= 45° C
IDP3
Reverse: 5' TTATAGTTTGCACATACCTT 3' Tm= 44° C
In-Frame Fusion Protein of His-6 IDP:
Histidine-Histidine-Histidine-Histidine-Histidine-Histidine-Methionine-Serine-Lysine-Isoleucine-Lysine-Valine-Valine-Histidine-Proline-Isoleucine
Coenzyme:
NADP+
For questions or comments contact elsellars@davidson.edu