*This page is part of undergraduate assignment for Molecular Biology at Davidson College

Cloning 5 Isoforms of Isocitrate Dehydrogenase

By Elizabeth Shafer

Orthologs

I did a BLASTp search of the first 40 amino acids of IDP2 to search for amino acid structural homologs. Below, I have described the nucleotide sequence, amino acid sequence, and reproduced a sequence alignment amongst five IDP2 orthologs.

Isocitrate Dehydrogenase from E.coli (IBL5).

Escherichia coli is enterobacteria. Its has a gene that encodes for isocitrate dehydrogenase.

For more information check:

Hurley JH, Thorsness PE, Ramalingam V, Helmers NH, Koshland DE Jr, Stroud RM.Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8635-9.

 Nucleotide Sequence CDS 1251 bp
   1 atggaaagta aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa
  61 ctcaacgttc ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat
 121 gtaaccccag ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag
  181 cgtaaaatct cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag
   241 gacgtctggc tgcctgctga aactcttgat ctgattcgtg aatatcgcgt tgccattaaa
  301 ggtccgctga ccactccggt tggtggcggt attcgctctc tgaacgttgc cctgcgccag
    361 gaactggatc tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg
  421 gttaaacacc ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat
  481 gcgggtatcg aatggaaagc agactctgcc gacgccgaga aagtgattaa attcctgcgt
  541 gaagagatgg gggtgaagaa aattcgcttc ccggaacatt gtggtatcgg tattaagccg
   601 tgttcggaag aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac
  661 gatcgtgact ctgtgactct ggtgcacaaa ggcaacatca tgaagttcac cgaaggagcg
  721 tttaaagact ggggctacca gctggcgcgt gaagagtttg gcggtgaact gatcgacggt
  781 ggcccgtggc tgaaagttaa aaacccgaac actggcaaag agatcgtcat taaagacgtg
  841 attgctgatg cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc
  901 tgtatgaacc tgaacggtga ctacatttct gacgccctgg cagcgcaggt tggcggtatc
  961 ggtatcgccc ctggtgcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt
  1021 actgcgccga aatatgccgg tcaggacaaa gtaaatcctg gctctattat tctctccgct
  1081 gagatgatgc tgcgccacat gggttggacc gaagcggctg acttaattgt taaaggtatg
  1141 gaaggcgcaa tcaacgcgaa aaccgtaacc tatgacttcg agcgtctgat ggatggcgct
  1201 aaactgctga aatgttcaga gtttggtgac gcgatcatcg aaaacatgta a
 
 Amino Acid Sequence 416 aa 
 MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
  MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
  LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
   AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
   SVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
  IADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
  FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
   FERLMDGAKLLKCSEFGDAIIENM
Isocitrate Dehydrogenase from Bacillus coagulans (2AYQ_B)

Bacillus coagulans is a bacillus bacteria and moderate thermophile. Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is involved in leucine biosynthesis at high temperatures.

For more information check:
Tsuchiya D, Sekiguchi T, Takenaka A.Crystal structure of 3-isopropylmalate dehydrogenase from the moderate facultative thermophile, Bacillus coagulans: two strategies for thermostabilization of protein structures.




J Biochem (Tokyo). 1997 Dec;122(6):1092-104.
 
 CDS 1101 bp
 1 atgaaaatga aactggccgt actgcccggc gatgggatcg ggccggaagt gatggatgca
 61 gcgatccgcg ttttaaaaac agtgttggac aatgacgggc atgaagccgt ttttgaaaat
 121 gcgctgattg ggggcgccgc cattgatgaa gcggggacgc ccctaccgga agaaacgctt
  181 gacatttgcc gcaggagcga tgccattttg ctcggcgcgg taggggggcc gaaatgggat
 241 cataacccgg cttccctccg cccggaaaaa ggcctgctcg ggctccggaa agaaatgggg
 301 ctgtttgcga acctgcgccc ggttaaagca tatgccacac ttttaaacgc atcgccttta
 361 aaacgggaac gtgtggaaaa cgtcgatctt gttattgtcc gcgaactgac gggcggcctc
 421 tattttgggc gcccgagtga aaggcgcggg ccgggcgaga atgaagtggt agacacgctt
  481 gcctatacaa gggaagagat tgaaagaatt attgagaaag cattccagct tgcccaaatc
 541 agaagaaaaa aactggcatc cgtcgataag gcgaatgtgc tggaatcaag cagaatgtgg
 601 cgcgaaattg cggaagaaac cgcgaaaaag tatccggacg tggaattgag ccatatgctt
 661 gtcgactcaa cttcgatgca gctgattgca aatccgggcc aatttgatgt cattgtaaca
 721 gagaatatgt tcggcgatat tttaagcgat gaagcgtccg tgattaccgg cagcctcggc
 781 atgttgccat ccgcaagcct ccgttccgac cggttcggca tgtatgaacc ggtccacggc
 841 tccgcgccgg atattgccgg gcagggaaaa gccaacccgc tcgggacagt gctgtcagcg
 901 gctttgatgc tccgttattc gttcgggctt gagaaagaag cggcggccat tgaaaaagca
 961 gtggatgatg tgcttcaaga cggctattgt acaggcgatt tgcaggtggc aaacggaaaa
 1021 gtggtcagta caattgagct cacagaccgg ctgatcgaaa aattaaataa cagcgcagcc
  1081 ggtccgcgca tttttcaata a
 Amino Acid Sequence 336 aa
 1 mkmklavlpg dgigpevmda airvlktvld ndgheavfen aliggaaide agtplpeetl
 61 dicrrsdail lgavggpkwd hnpaslrpek gllglrkemg lfanlrpvka yatllnaspl
 121 krervenvdl vivreltggl yfgrpserrg pgenevvdtl aytreeieri iekafqlaqi
 181 rrkklasvdk anvlessrmw reiaeetakk ypdvelshml vdstsmqlia npgqfdvivt
  241 enmfgdilsd easvitgslg mlpsaslrsd rfgmyepvhg sapdiagqgk anplgtvlsa
 301 almlrysfgl ekeaaaieka vddvlqdgyc tgdlqvangk vvstieltdr lieklnnsaa
 361 rprifq
Isocitrate Dehydrogenase from Thermus thermophilus (1XAA).

Thermus thermophilus is an extremophile. Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is involved in leucine biosynthesis at high temperatures.

For more information check:

Yamada T, Akutsu N, Miyazaki K, Kakinuma K, Yoshida M, Oshima T.Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus strain HB8.
J Biochem (Tokyo). 1990 Sep;108(3):449-56.

 
 
 CDS 1488 bp
 1 atgcccctga tcaccacgga aaccggcaag aagatgcacg ttctcgagga cgggcgcaag
 61 ctcatcaccg tcatccccgg agacggcatc gggcccgagt gcgtggaggc taccctcaag
 121 gtcctagagg cggccaaggc ccccctggcc tacgaggtgc gagaggcggg ggcgagcgtc
 181 ttccggcggg gcatcgcctc gggcgttccc caggagacca ttgagtccat ccgcaagacc
  241 cgggtggtcc tgaagggtcc cctggaaacc ccggtgggct acggggagaa gagcgccaac
 301 gtcaccctaa ggaagctctt tgagacctac gccaacgtcc gccccgtgcg ggagttcccc
 361 aacgtcccca ccccctatgc gggccggggc attgacctcg tggtggtgcg ggagaacgtg
 421 gaggacctct acgccgggat tgagcacatg cagaccccga gcgtggccca gaccctcaag
 481 ctcatctcct ggaagggatc ggagaagatc gtccgcttcg cctttgagct ggcccgggcc
 541 gaggggcgga agaaggtcca ctgcgccacc aagtccaaca tcatgaagct cgccgaagga
  601 cccaagcggg cctttgagca ggtggcccag gagtaccccg acatagaagc ggtccacatc
  661 atcgtggaca acgctgccca ccagctggtg aaaaggcccg agcagtttga ggtgatcgtc
  721 accaccaaca tgaacggaga catcctctcc gacctcacct cggggctcat tgggggcctg
  781 ggcttcgctc cctcggccaa catcggcaac gaggtggcca tctttgaggc cgtccacggt
  841 tccgccccca agtacgccgg gaagaacgtc atcaacccca ccgcggtcct cctctcggcg
  901 gtgatgatgc tccgctacct ggaggagttc gccacggcgg accttataga gaacgccctc
  961 ctctacaccc tcgaggaggg ccgggtcctc acgggggacg tggtgggcta cgaccggggg
  1021 gccaagacca cggagtacac cgaggccatc atccagaacc tgggcaagac cccaaggaag
  1081 acccaggtgc ggggctacaa gcccttccgc ctgccccagg tggacggggc catcgccccc
  1141 atcgtcccta ggagccgccg ggttgtgggg gtggacgtct tcgtggaaac caacctcctg
  1201 cccgaggccc tgggaaaggc cctggaggac cttgccgcgg gcaccccctt ccggctcaag
  1261 atgatctcca accggggcac ccaggtctac ccccccaccg gcgggctcac ggacctggtg
  1321 gaccactacc gctgccgctt cctctacacg ggggaggggg aggctaagga cccggagatc
  1381 ctggacctcg taagccgggt ggcaagccgc ttccgctgga tgcacctgga gaagctccag
  1441 gaatttgacg gcgagcccgg cttcaccaag gcccaagggg aagactaa
 Amino Acid Sequence 345 aa
 1 mkvavlpgdg igpevteaal kvlraldeae glglayevfp fggaaidafg epfpeptrkg
  61 veeaeavllg svggpkwdgl prkirpetgl lslrksqdlf anlrpakvfp glerlsplke
   121 eiargvdvli vreltggiyf geprgmseae awnteryskp evervarvaf eaarkrrkhv
  181 vsvdkanvle vgefwrktve evgrgypdva lehqyvdama mhlvrsparf dvvvtgnifg
  241 dilsdlasvl pgslgllpsa slgrgtpvfe pvhgsapdia gkgianptaa ilsaammleh
 301 afglvelark vedavakall etpppdlggs agteaftatv lrhla
Isocitrate Dehydrogenase from Brassica napus (gi 126201)
Brassica napus is a flowering plant. Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is a chloroplast precusor. 
 For more information check: 
Ellerstrom M, Josefsson LG, Rask L, Ronne H.Cloning of a cDNA for rape chloroplast 3-isopropylmalate dehydrogenase by genetic complementation in yeast.




Plant Mol Biol. 1992 Feb;18(3):557-66.
 
 CDS 1221 bp
 1 atggcggcgg ctctgcagac taacatccga ccggttaagt ttccggctac gttgagagct
 61 ctcaccaaac aatcttctcc agcacccttt agagtgagat gcgccgctgc ttcccccggg
  121 aaaaagagat acaatatcac tctccttccc ggcgatggaa tcggtccgga ggtcatctcc
  181 atcgctaaaa atgtgcttca gcaagctggt tccttggaag gtctggagtt tagcttccag
  241 gaaatgcctg taggaggagc tgctttggat ttggtcggag tgcctttgcc tgaggagacc
  301 gtctcggctg ctaaagaatc agatgctgtg cttcttggag ccattggagg gtacaaatgg
  361 gataagaatg aaaaacattt gaagcctgag actgggttac ttcaacttcg ggctggtctt
  421 aaagtctttg ctaatctgag acctgctaca gttcttccac agttagtgga tgcttcgacc
  481 ttgaagagag aggttgcaga aggtgttgat ctgatggttg ttagggagct tacaggaggt
  541 atttactttg gagtgccaag gggcattaag actaatgaaa atggtgagga agttgggtat
  601 aataccgagg tctatgctgc tcacgagatt gatagaattg ctcgtgttgc cttcgagact
  661 gctcggaaac ggcgtggcaa gctgtgttct gttgacaaag ctaatgtctt agatgcctcg
  721 attttatgga ggagacgagt aacagcacta gctgctgaat atccggatgt tgaactgtca
  781 catatgtatg ttgacaatgc tgccatgcag cttgttcgtg accctaaaca gtttgacacc
  841 attgttacaa acaacatttt tggtgatata ttatccgatg aagcgtcgat gatcacagga
  901 agcatcggca tgcttccctc tgctagtctc agtgattcgg gacctggact ctttgaacct
  961 atacatggtt ctgcacctga tattgctgga caggataaag caaacccgtt ggcaaccatc
  1021 ctcagcgctg caatgcttct gaaatacgga ctcggagagg agaaggcagc taagagaatc
  1081 gaagacgctg tgttgggtgc tctgaacaaa ggattcagaa caggagacat ctactccgca
  1141 ggaactaaac ttgtgggctg caaggagatg ggagaggaag ttctgaagtc agtggattcc
  1201 cacgttcaag cttctgttta a
 Amino Acid Sequence 406 aa
 1 maaalqtnir pvkfpatlra ltkqsspapf rvrcaaaspg kkrynitllp gdgigpevis
  61 iaknvlqqag sleglefsfq empvggaald lvgvplpeet vsaakesdav llgaiggykw
  121 dknekhlkpe tgllqlragl kvfanlrpat vlpqlvdast lkrevaegvd lmvvreltgg
  181 iyfgvprgik tnengeevgy ntevyaahei driarvafet arkrrgklcs vdkanvldas
 241 ilwrrrvtal aaeypdvels hmyvdnaamq lvrdpkqfdt ivtnnifgdi lsdeasmitg
  301 sigmlpsasl sdsgpglfep ihgsapdiag qdkanplati lsaamllkyg lgeekaakri
  361 edavlgalnk gfrtgdiysa gtklvgckem geevlksvds hvqasv
Isocitrate Dehydrogenase from Neurospora crassa  (gi_462502)
Neurospora crassa is an eukaryotic fungi. Its homolog for isocitrate dehydrogenase encodes a 3-Isopropylmalate dehydrogenase which is involved in leucine biosynthesis. 
For more information check: 




Li Q, Jarai G, Yaghmai B, Marzluf GA. The leu-1 gene of Neurospora crassa: nucleotide and deduced amino acid sequence comparisons. Gene 1993 Dec 22;136(1-2):301-5
 
  CDS 1107 bp
 1 atggctactc ataacattgt tgtgttcggt ggtgaccact gcggtcccga ggttgttctc
  61 gaggccatca aggtcctcaa ggcgatcgag accaacagcc cttcggcgtg caagttcaac
  121 ctccagaacc acctccttgg cggtgcctcc atcgacaagc acaatgaccc cctcaccgat
  181 gaggccctca acgccgccaa ggctgccgat gccgtccttc tcggtgccat tggcggtccc
  241 gaatggggca cctcttccac cgtccgcccc gagcaaggtc tcctgaagct ccgcaaggag
  301 ctcggcacct atggcaacct tcgcccttgc aactttgctt ccgagtccct cgtcgacagc
  361 tctcccctca aggccgaggt ctgccgcggc actgacttca ttgtcgtccg tgagcttacc
  421 ggtggtatct actttggtga ccgcaccgag gatgacggct ccggctacgc ctgcgatacc
  481 gagccctaca gccgcgccga gatcgtgcgc atcgccagac tcgccggctt cctcgccctg
  541 gccaagaact ctcccgccaa ggtctggtct ctggacaagg ccaacgtgct cgccaccagc
  601 cgcctctggc gcaagactgt gaccgacgtc attagcaagg agtgccccca gcttcagctc
  661 gagcaccagc tcatcgacag cgccgccatg ctgctcgtca agaacccccg tgccctcaac
  721 ggcgtcgtca ttaccagcaa cctctttggc gacatcatct cggacgaggc ctcggtcatc
  781 cccggctcca tcggcctgct cccttccgcc agctggggcg gaatccccga cggcaaggtc
  841 aagtgcaacg gcatttacga gcccatccac ggctccgctc ccgatatttc gggtaagggc
  901 atcgtcaacc ccgtctgtac cattctctcc gtcgccatga tgctccgcta ctcgctcaac
  961 ctccccaagg aggccgatgc cgttgaggct gctgtcaagg cagccattga caacggtacc
  1021 aagaccaagg accttggcgg caacgctact acttcggata tgggtaacgc tgtagttgct
  1081 gagttggaga agatccttaa ggcttaa
 Amino Acid Sequence 368 bp
  1 mathnivvfg gdhcgpevvl eaikvlkaie tnspsackfn lqnhllggas idkhndpltd
   61 ealnaakaad avllgaiggp ewgtsstvrp eqgllklrke lgtygnlrpc nfaseslvds
  121 splkaevcrg tdfivvrelt ggiyfgdrte ddgsgyacdt epysraeivr iarlagflal
  181 aknspakvws ldkanvlats rlwrktvtdv iskecpqlql ehqlidsaam llvknpraln
  241 gvvitsnlfg diisdeasvi pgsigllpsa swggipdgkv kcngiyepih gsapdisgkg
  301 ivnpvctils vammlrysln lpkeadavea avkaaidngt ktkdlggnat tsdmgnavva
  361 elekilka
 
Amino Acid Sequence Alignment:
 

 10 20 30 40 50 60
 ....*....|....*....|....*....|....*....|....*....|....*....|
consensus  1 KIALLPGDGIGPEVTEAALKVLKAALEKAP---LEFEFEEYLVGGAAIDATG--EPLPDE 55
1BL5  26 IIPYIEGDGIGVDVTPAMLKVVDAAVEKAYkgeRKISWMEIYTGEKSTQVYGqdVWLPAE 85
2AYQ_B  4 KLAVLPGDGIGPEVMDAAIRVLKTVLDNDG---HEAVFENALIGGAAIDEAG--TPLPEE 58
1XAA  2 KVAVLPGDGIGPEVTEAALKVLRALDEAEG---LGLAYEVFPFGGAAIDAFG--EPFPEP 56
gi 126201  45 NITLLPGDGIGPEVISIAKNVLQQAGSLEG---LEFSFQEMPVGGAALDLVG--VPLPEE 99
gi 462502  5 NIVVFGGDHCGPEVVLEAIKVLKAIETNSPs-aCKFNLQNHLLGGASIDKHN--DPLTDE 61

 70 80 90 100 110  120
 ....*....|....*....|....*....|....*....|....*....|....*....|
consensus  56 TLEACRKADAVLKGAVGGPKWd--pGEVRPENglLALRKELDLYANLRPVKVYp-aLGDK 112
1BL5  86 TLDLIREYRVAIKGPLTTPVG----GGIRSLN--VALRQELDLYICLRPVRYY---QGTP 136
2AYQ_B  59 TLDICRRSDAILLGAVGGPKwdhnpASLRPEKglLGLRKEMGLFANLRPVKAYa-tLLNA 117
1XAA  57 TRKGVEEAEAVLLGSVGGPKwdglpRKIRPETglLSLRKSQDLFANLRPAKVFp-gLERL 115
gi 126201 100 TVSAAKESDAVLLGAIGGYKwdkneKHLKPETglLQLRAGLKVFANLRPATVLp-qLVDA 158
gi 462502  62 ALNAAKAADAVLLGAIGGPEWgt-sSTVRPEQglLKLRKELGTYGNLRPCNFAsesLVDS 120

 130 140 150 160 170 180
 ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 113 SPLKNEvvEGVDIVIVRELTGGIYFGIEKGIDG-----------------SGN-GEEVAV 154
1BL5 137 SPVKHP--ELTDMVIFRENSEDIYAGIEWKADSadaekvikflreemgvkKIRfPEHCGI 194
2AYQ_B 118 SPLKRErvENVDLVIVRELTGGLYFGRPSER-------------------RGPgENE-VV 157
1XAA 116 SPLKEEiaRGVDVLIVRELTGGIYFGEPRGMS-----------------------EAEAW 152
gi 126201 159 STLKREvaEGVDLMVVRELTGGIYFGVPRGIKT-----------------NEN-GEEVGY 200
gi 462502 121 SPLKAEvcRGTDFIVVRELTGGIYFGDRTEDDG-----------------SG-----YAC 158

 190 200 210 220 230 240
 ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 155 DTKLYSRDEIERIARAAFELARKRG-RKKVTSVDKANVLKSSD----LWREIVAEVAAK- 208
1BL5 195 GIKPCSEEGTKRLVRAAIEYAIAND-RDSVTLVHKGNIMKFTEgafkDWGYQLAREEFGg 253
2AYQ_B 158 DTLAYTREEIERIIEKAFQLAQIR--RKKLASVDKANVLESSR----MWREIAEETAK-- 209
1XAA 153 NTERYSKPEVERVARVAFEAARKR--RKHVVSVDKANVLEVGE----FWRKTVEEVGR-- 204
gi 126201 201 NTEVYAAHEIDRIARVAFETARKR--RGKLCSVDKANVLDASI----LWRRRVTALAA-- 252
gi 462502 159 DTEPYSRAEIVRIARLAGFLALAKNsPAKVWSLDKANVLATSR----LWRKTVTDVISK- 213

 250 260 270 280 290  300
 ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 209 ---------------EYPDIELEHMLVDNAAMQLVKNP-KQFDVIVTPNLFGDILSDEAS 252
1BL5 254 elidggpwlkvknpnTGKEIVIKDVIADAFLQQILLRP-AEYDVIACMNLNGDYISDALA 312
2AYQ_B 210 ---------------KYPDVELSHMLVDSTSMQLIANP-GQFDVIVTENMFGDILSDEAS 253
1XAA 205 ---------------GYPDVALEHQYVDAMAMHLVRSP-ARFDVVVTGNIFGDILSDLAS 248
gi 126201 253 ---------------EYPDVELSHMYVDNAAMQLVRDP-KQFDTIVTNNIFGDILSDEAS 296
gi 462502 214 ---------------ECPQLQLEHQLIDSAAMLLVKNPrALNGVVITSNLFGDIISDEAS 258

 310 320 330 340 350 360
 ....*....|....*....|....*....|....*....|....*....|....*....|
consensus 253 MLTGSLGMLPSASLGPDGf------ALFEPVHGSAPDIAGKDKANPIATILSAAMMLRHs 306
1BL5 313 AQVGGIGIAPGANIGDEC-------ALFEATHGTAPKYAGQDKVNPGSIILSAEMMLRH- 364
2AYQ_B 254 VITGSLGMLPSASLRSdr------fGMYEPVHGSAPDIAGQGKANPLGTVLSAALMLRYs 307
1XAA 249 VLPGSLGLLPSASLGrg-------tPVFEPVHGSAPDIAGKGIANPTAAILSAAMMLEHa 301
gi 126201 297 MITGSIGMLPSASLSDsg------pGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYg 350
gi 462502 259 VIPGSIGLLPSASWGGIPdgkvkcnGIYEPIHGSAPDISGKGIVNPVCTILSVAMMLRYs 318

 370 380 390 400
 ....*....|....*....|....*....|....*....|....*..
consensus 307 LGLED-AaDAIEAAVLKTLEAGIRTKDLAGNAD--KYVSTSEFGDAV 350
1BL5 365 MGWTEaA-DLIVKGMEGAINAKTVTYDFERLMDgaKLLKCSEFGDAI 410
2AYQ_B 308 FGLEK-EaAAIEKAVDDVLQDGYCTGDLQVANG--KVVSTIELTDRL 351
1XAA 302 FGLVE-LaRKVEDAVAKALLETP-PPDLGGSAG--TEAFTATVLRHL 344
gi 126201 351 LGEEK-AaKRIEDAVLGALNKGFRTGDIYSAGT--KLVGCKEMGEEV 394
gi 462502 319 LNLPK-EaDAVEAAVKAAIDNGTKTKDLGGNA------TTSDMGNAV 358

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Created by: Elizabeth Shafer. Email questions to lishafer@davidson.edu