This web page was created as an assignment for an undergraduate course at Davidson College.

Structure of Isocitrate Dehydrogenase

This page requires the Rasmol plugin. Download a free copy of Rasmol here.

Figure 1: Isocitrate dehydrogenase isolated from Bacillus subtilis. There are two protein chains that can easily be seen by changing the color of the image to amplify the chains. This structure is NADP(+)-dependent. MMDB Id: 16922 PDB Id: 1HQS

Authors: S.K.Singh, K.Matsuno, D.C.Laporte & L.J.Banaszak

PDB Title: "Crystal Structure Of Isocitrate Dehydrogenase From Bacillus Subtilis"

Full article at http://www.jbc.org/cgi/content/full/276/28/26154

Figure 2: E. coli isocitrate dehydrogenase complex with NADP(+). MMDB Id: 3431 PDB Id: 9ICD

Contrary to the original caption of this chime image, it does not contain NADP(+).

Authors: J.H.Hurley, A.M.Dean, D.E.Koshland Junior & R.M.Stroud

Abstract located here

The alpha subunit in E. coli is coded by a sequence similar to the yeast IDH2 sequence. See also Orthologs.

Figure 3: NAD(+)-dependent 3-isopropylmalate dehydrogenase from Thermus thermophilus. MMDB Id: 1160 PDB Id: 1HEX

Authors: J.H.Hurley

Abstract located here

Although we are not studying 3-isopropylmalate dehydrogenase, Figure 3 is the only protein that has been isolated and crystallized in its NAD(+)-dependent form. This protein is in the same family and is very similar to the isocitrate dehydrogenase proteins and may give some insight into what these proteins may look like three dimensionally.

I have also included the sequences of the IDH1, IDH2, IDP1, IDP2, and IDP3 genes from Saccharomyces cerevisiae in these webpages and you can find them here. While the sequences located on these webpages are specific for yeast, some may have orthologs in other species. To see the orthologs for IDH2, click Orthologs.

© Copyright 2002 Department of Biology, Davidson College, Davidson NC 28035

Send comments, questions, and suggestions to vistatler@davidson.edu