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Restriction Fragments

The following fragment sizes will be used to determine the correct orientation of the insert (Cherry et al., 2000).

IDH1

Restriction enzyme: EcoR V

Recognition sequence: GATATC

Plasmid: Cuts 16 bp from the insertion point

Insert: Cuts at bp 340

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IDH2

Restriction enzyme: Bgl II

Recognition sequence: AGATCT

Plasmid: Cuts 15 bp from the insertion point

Insert: Cuts at bp 335

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REVERSE:

IDP1

Restriction enzyme: Bgl II

Recognition sequence: AGATCT

Plasmid: Cuts 15 bp from the insertion point

Insert: Cuts at bp 1208

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REVERSE:

IDP2

Restriction enzyme: Bgl II

Recognition sequence: AGATCT

Plasmid: Cuts 15 bp from the insertion point

Insert: Cuts at bp 496

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REVERSE:

IDP3

Restriction enzyme: Cla I and Bgl II

Recognition sequences: Bgl I -- AGATCT; Cla I -- ATCGAT

Plasmid: Bgl I cuts 15 bp from the insertion point

Insert: Cla I cuts at bp 768

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REVERSE:

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