Gene Networks Database

LOCUS       Rr_SPEC2A     348 bp    DNA             INV       20-MAY-1998
DEFINITION  Strongylocentrotus purpuratus aboral ectoderm-specific Spec2a
            gene, sequence of the 348-bp XhoI fragment, containing RSR enhancer 
KEYWORDS    
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Mao, C. A., Gan, L., Klein, W. H.
    TITLE   Multiple Otx binding sites required for expression of 
            the Strongylocentrotus purpuratus Spec2a gene.
  JOURNAL   Dev. Biol. 165 (1): 229-242 (1994)   
  MEDLINE   94374572
REMARK      GeNet staff at the IHC&DB created this entry from the original
            journal article. This sequence comes from Fig. 1B
COMMENTS    The RSR enhancer is a 188-bp SstI-XhoI between base pairs
            -631 and -443 (154-342 of this entry) in the Spec2a control region.
            The RSR enhancer appears tp be a key regulatory region controlling
            Spec2a gene transcription during embryogenesis. Three DNA elements
            with the consensus sequence TAATCC/T are positive cis-regulatory elements
            essential for full reporter gene expression in sea urchin embryos.
            Because the sea urchin orthodenticle-related protein SpOtx binds
            these TAATCC/T elements with high affinity, they have been designated
            Otx sites. These sites, and most likely a fourth just upstream of the
            RSR enhancer, appear to act additively to confer positive activity to
            the Spec2a control region [1]. 
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
     protein_bind    111..116
                     /citation=[1]
                     /bound_moiety="SpOtx"
                     /evidence=bandshift analysis
     protein_bind    185..190
                     /citation=[1]
                     /bound_moiety="SpOtx"
                     /evidence=bandshift analysis
     protein_bind    276..281
                     /citation=[1]
                     /bound_moiety="SpOtx"
                     /evidence=bandshift analysis
     protein_bind    332..337
                     /citation=[1]
                     /bound_moiety="SpOtx"
                     /evidence=bandshift analysis
ORIGIN
       1  CTCGAGAGGA TCAGACCTTT CTGATTTTGG AATGAATGAG GGGTGTTGGC CACGAAGGTG 
      61  AATTTTCCAG CCACAGCTAG GCTACAATCC ATCGTGAGCA ATAAAAACTC TAATCCATCC 
     121  CCTGAAAATT TTAATCGCAC CGCCCCCATT TGTGAGCTCC TTTCAAAGAG CACAACAAGC 
     181  CCTCAGATTA ACATTCCATC TCATTCAAAA ATAGCAGACA GCTTGATATT ATGGGATGAT 
     241  ACCCACATTG TTGAGTTGTC CATCTAAAAA GGATTTAATC CTTCACTGAA TTGGTGCTCT 
     301  TGCTATACAT TGTTACCTTT CTTTGCTCCA GTAATCTCAA GCCTCGAG