Gene Networks Database

LOCUS       Pr_SPSPEC1      640 bp    DNA             INV       28-SEP-1998
DEFINITION  Strongylocentrotus purpuratus Spec1 proximal region.
DBSOURCE    GENBANK:  name SPSPEC11, accession X03287
KEYWORDS    calcium binding protein; spec1 gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Hardin,S.H., Carpenter,C.D., Hardin,P.E., Bruskin,A.M. and
            Klein,W.H.
  TITLE     Structure of the Spec1 gene encoding a major calcium-binding
            protein in the embryonic ectoderm of the sea urchin,
            Strongylocentrotus purpuratus
  JOURNAL   J. Mol. Biol. 186 (2), 243-255 (1985)
  MEDLINE   86115267
REFERENCE   2  (SpF1 binding site)
  AUTHORS   Hardin,S.H., Carpenter,C.D., Hardin,P.E., Bruskin,A.M. and
            Klein,W.H.
  TITLE     Ectoderm nuclei from sea urchin embryos contain a Spec-DNA 
            binding protein similar to the vertebrate transcription factor
            USF. 
  JOURNAL   J. Mol. Biol. 186 (2), 243-255 (1985)
  MEDLINE   91184014   
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
     promoter        347..355
                     /note="CAAT sequence"
     promoter        386..392
                     /note="TATA sequence"
     misc_feature    415
                     /note="transcription initiation site"
     protein_bind    453..474                    
                     /bound_moiety="SpF1" 
                     /evidence=band shift gel assays,
                     /evidence= DNase I footprinting                                          
                     /citation=[2]

BASE COUNT      189 a    146 c    114 g    191 t
ORIGIN
        1 gacattacga ccaatgaaac tttattttgg ttatcaaatg cttttttcgt gctcggatag
       61 ctctactgaa gacgaaacca tttcaaccag caaaatcgac caaggaaatc atgagttgga
      121 gcgaatgtaa ggttttcatc atcatgattt tctcatctaa ttattgacag tttccgaaaa
      181 tttccgaaat ttgtatatag cacggtcaca ccggcaaagc aaaagccgcc gtgctcacag
      241 gtgcatgcat gaataatgat tacataactt tcgtgagcat atatccagaa ttagtctcag
      301 tgggttttac aattttgttc aagtgacctt tatctgcgca tgcacagatc aatccgcgca
      361 tgctcagatt gtatctacga actggtatat ttgttagaac tgcaacccca cactacgcct
      421 aatacgcctt aaacatcagc aacgacattc ctagcgtatg gtcacgtgct ttatttcagg
      481 agttatcact ctttcttgaa taatactctt cgtcagcact ccacccttac cactccttga
      541 aagcaagtcg actagaagag aagtataggt acctgagctc cagaggaatt ttctatcttc
      601 aattttgccg gatctatccc aacttccaaa gatggtaagt