LOCUS SPU18784 3285 bp mRNA INV 02-FEB-1996 DEFINITION Strongylocentrotus purpuratus transcription factor SpGCF1 mRNA, complete cds. ACCESSION U18784 NID g755247 KEYWORDS . SOURCE purple urchin. ORGANISM Strongylocentrotus purpuratus Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata; Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida; Strongylocentrotidae; Strongylocentrotus. REFERENCE 1 (bases 1 to 3285) AUTHORS Zeller,R.W., Coffman,J.A., Harrington,M.G., Britten,R.J. and Davidson,E.H. TITLE SpGCF1, a sea urchin embryo DNA-binding protein, exists as five nested variants encoded by a single mRNA JOURNAL Dev. Biol. 169 (2), 713-727 (1995) MEDLINE 95301110 REFERENCE 2 (bases 1 to 3285) AUTHORS Zeller,R.W. TITLE Direct Submission JOURNAL Submitted (17-DEC-1994) Robert W. Zeller, University of California, San Diego, Biology, 9500 Gilman Drive, La Jolla, CA 92093-0347, USA FEATURES Location/Qualifiers source 1..3285 /organism="Strongylocentrotus purpuratus" /db_xref="taxon:7668" /dev_stage="4 hr embryo" CDS 108..1568 /codon_start=1 /function="transcription factor" /product="SpGCF1" /db_xref="PID:g755248" /translation="MSTLPQPLSHCLLNQVHPALNLPQTGVITDIKPMISNKPPTQEV KPNILATGLPYPPLNVPRLPVMPNVSLPSVSMPSVSMPNVSMPNASMPSVSMPNVSMP SIPHHNLQGNLGQLLNNSNSQKMSQMKKCPNEFLHQNPQSERQLFYNDVAMQLYNSDF NKFASKKGFHGYLLEQQKWRWDTHSYIGNLETRVHNLLINPNSGVAQNVARYRSVPIK CKSEDVKRCKATSKELENMATRIASVRQQLLHKKGTLLTSSDNSVIVWQNELAYIEQL FDRTDQMYNEVLSTLASVNQTFSHLQTSFTAEAAELADRRRLWRRRKENNRKRRKRME KQLEKIEQRSCELLFHITSRGAYDRVRSHPEMPRIGPSEVNTDMLNGIKSKSEVRPLM HLLSKGYMTPGAMEMVSQKIQKLECGIKTEAHQQATQVGINSLSINKITAPASELNSI LPPVTGIASSNMVSSVNSAVTQQSVPTVNLNTQLAK" polyA_signal 1949..1954 polyA_signal 2564..2569 polyA_signal 3093..3098 BASE COUNT 973 a 695 c 647 g 970 t ORIGIN 1 ggattttggg ggcataattt tgctattgat caaggatagc gggcccgaat ttactcattt 61 tttagtgact tgacgaggat ccaacagagg tgagtgagga gtgaggtatg tccactctgc 121 cccagcccct ttcccactgc ctgctgaacc aggtacaccc cgctctcaac ctgccccaga 181 caggggtcat cacagacatc aagcccatga tcagtaataa acctcctaca caggaggtca 241 aaccaaacat cctagcaact ggcttgccct atcctccact caacgtgcct aggctacccg 301 tcatgcccaa tgtgtctctg cctagtgtct ctatgccgag tgtgtctatg cccaatgtct 361 ccatgcccaa cgcatccatg cccagtgttt cgatgcccaa tgtgtccatg ccaagtattc 421 ctcatcacaa cttacagggt aacttaggcc aattactcaa caacagtaat tcccaaaaaa 481 tgtcccaaat gaaaaagtgc cccaacgagt ttttacatca gaatccacaa agtgagcgac 541 agcttttcta caacgacgta gccatgcaac tgtataacag tgacttcaac aagtttgctt 601 ccaagaaggg atttcatggc tacctgttag agcaacagaa gtggaggtgg gatacccaca 661 gctacatagg taacctggag actagagtac ataacttgct cattaatcca aacagtgggg 721 ttgcacagaa tgttgctcgc taccgcagtg tccccatcaa atgtaaaagt gaggatgtga 781 agcgatgtaa agccacgtcc aaagagcttg agaacatggc aacccgtatt gccagtgtac 841 ggcagcagct gctacacaaa aagggcacct tgctgacatc cagcgataac agcgttatag 901 tgtggcagaa tgagctagcc tacatagaac agctgtttga caggactgat cagatgtaca 961 atgaggtgtt atctaccctg gcaagtgtca accagacctt ctcccacctt cagacaagct 1021 tcacagcaga agctgcagag ttggcagatc gtaggcgctt gtggaggagg agaaaggaga 1081 acaaccgcaa gagacgcaag cgcatggaga aacaacttga aaagattgag cagcgatctt 1141 gtgagcttct ctttcatatc acatcccggg gagcatatga ccgggtgcgt tcccacccag 1201 agatgcctcg tattggaccc agcgaggtga acacagacat gttaaatggg attaaatcta 1261 aatccgaagt gaggcctctt atgcacctac tcagtaaggg ttacatgacc cctggtgcaa 1321 tggagatggt ctctcaaaag atccaaaaac tagagtgtgg tattaagact gaagcgcacc 1381 aacaggcaac ccaggttggt atcaactccc tgtcgatcaa caaaattaca gcacctgctt 1441 cagagctaaa ctccatactg cctcctgtca ctggaattgc ctcatcaaat atggtgtcat 1501 ctgtaaactc agctgtgaca caacaatcag tgcccacagt aaatcttaac actcaattag 1561 cgaagtaaag acattttaac caagtcacag cgactttgcc acattgccga gtgtttgaca 1621 ttgagtaggc tgtactctac tccacactgt tttaaccaac attgtattat gtatgagcat 1681 actcttacat ggcaaaatgt acatttatta tattatgcag gaatgttgca tcagtttaga 1741 tctaaccaaa acagttttag attacttgtt ctttttttta ccaggtgtac atgtaaattt 1801 taactggtga aatttcactt cgcttgaact ctttaaaaaa tcacaatctt cttcatccta 1861 ctagtaatat acatgtagga tggcccacac ttgggaaaga catcatttac caaattccct 1921 aaagctgtaa tcaaactcat tgcggtggaa taaaaattga ttacttgcct tgccacatat 1981 attacatgaa tcgaattgta agaaattgtc ctgttacttt gttctttatt gatcatgtct 2041 aggactgtcc aaattcaatg gcttagttta tcctggatcg gtgcttaatc ttagactttc 2101 ctcttggctg atttgtcact gattcattgt gtttgtaagt tgtaatagag ctgtagggta 2161 aatctcttct cttttttttt ggggggggtg gaggggaatg ggttgtttgg ggggtgttta 2221 tgttgacttt tccccccata atattccttc atggaccaaa tgtatagaac aattactacc 2281 cgttattaca tgtacatttg tcagtctctt aatgttattt tataatgctt tatttttagg 2341 cagctagagt agaatagttt ctgcattctg gacatttgtt ttactttgaa atatattatg 2401 ttcattcttt ttatcatagc cacttacccg ctacctcgga agtagccagt ctgagacctg 2461 gatttttaaa catggtttaa agaaatgggt gagatatttt aagtgtacca atcatccgaa 2521 aaaaaatgat ctgtgtagtg taaagctaga atgtataccg gtaaataaac tcaaccaaaa 2581 atatttacca ggcattatat aggtaaaccg attaaggagt attaaaaaaa atgaagggta 2641 atattaaatc cctcgtgcag ggcttgcaca gaacctaaat ccgacttagt tccgtgcgag 2701 ccctattcat gcaagtacgt acgtgtgcgt ttactactgg tagtctactc aacaagacga 2761 tatactgttg tatttaactt gcaatatctc tttaataata gaagtaatat ttgaagctca 2821 tattttttta atacattata gccacacatt gaacccctta catcaagagc aaaatttgtt 2881 ttagtgattg gtacactgta aacatttttg ttgttgctga gttcttaata agtgctactt 2941 gatctttgcc atttccttta tggtgaaatc attcaaacta tgtgattacc agtaaccttc 3001 caatcaagtt gaaccatgaa atatggtttg tattgctcgt ctatcattca aattattcag 3061 aatgtgatgg tcatatttca gctccaaaac gaaataaatc gggtcactca agcattgaag 3121 ttgagccatt gctttggctt tagggtgtaa atgattgctt aattttttac taatgattca 3181 atgtttttga caaagcatta aaatcaacag gttcaaattt gcttctggtg tgtaaatcta 3241 attatttatg acaacactaa attttgtaca agtctgaatc aagcc //