Gene Networks Database

SpOct - alpha H2B interaction

• REGULATOR: SpOct
• TARGET: alpha H2B
• INTERACTION TYPE: protein-DNA
• INTERACTION RESULT: activation
• TISSUE:
• STAGE: blastula
• TARGET SITE: ATGCAAAT
• PROOFS: in vitro binding of SpOct to alpha H2B wild-type promoter
• REFERENCES: Bell et al., 1992
• COMMENTS:It was shown by gel shift and methylation interference assays that incubation of an extract of blastula-stage sea urchin embryonic nuclei with a radiolabeled DNA-probe spanning the octamer site of the early H2B gene leads to formation of closely grouped set of protein DNA-complexes which were greatly reduced in amount by competition with a 17-bp oligonucleotide bearing the octamer site, but not by competition with a 20-bp oligonucleotide bearing the early H2B CCAAT element (Bell et al., 1992). The octamer element has a direct role in the expression of the alpha H2B gene and its activity is essential for the viability of the embryo (Char et al., 1993). A cloned sea urchin octamer-binding factor, SpOct, bound avidly to the wilt-type early H2B promoter fragment, but weakly to a fragment bearing a mutation in the octamer site (Bell et al., 1992).