Gene Networks Database

LOCUS       Rr_SPCYCB        538 bp    DNA             INV       14-MAY-1998
DEFINITION  Strongylocentrotus purpuratus cyclin B gene, 5' regulatory region
DBSOURCE    GENBANK:  name S80441, accession S80441
KEYWORDS    .
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Thatcher,J.D., McBride,B. and Katula,K.S.
  TITLE     Promoter binding factors regulating cyclin B transcription in the
            sea urchin embryo
  JOURNAL   DNA Cell Biol. 14 (10), 869-881 (1995)
  MEDLINE   96027756
COMMENTS    DNase I footprinting of the cyclin B upstream region revealed eight
            binding regions within 435 bp of the start of transcription; seven
            of these sites are within 215 bp. Found within these regions were
            consensus sequences for two CCAAT boxes, TATA  and E-boxes, and sequences
            with some similarity to E2F and octamer binding motifs. Upstream
            sequences were functionally defined by generating cyclin B-CAT fusion
            genes, containing deletions and base specific mutations, and testing
            for relative levels of expression by gene transfer. Both CCAAT boxes
            were found to be essential for maximal levels of expression. A third
            binding site (PR7) with no recognizable consensus sequence was also
            found to act as a positive element. Experiments show that protein
            binding to the E2F-like sequences may act to reduce expression.
            Sequence comparisons and methylation interference data indicate
            that the likely protein binding the CCAAT sequences is the characterized
            CCAAT-binding protein CP1. The results indicate that cyclin B is subject
            to positive and negative regulation, involving multiple factors that
            bind between -200 and -90 bp from the start of transcription [1].
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
     mRNA            442..
     protein_bind    7..40
                     /citation=[1]
                     /evidence=DNaseI footprinting
                     /site="PR8"
                     /note="octamer element"
     protein_bind    224..247
                     /citation=[1]
                     /evidence=DNaseI footprinting
                     /site="PR7"
     protein_bind    272..289
                     /citation=[1]
                     /site="PR6"
                     /evidence=DNaseI footprinting
                     /note="CCAAT box"
     protein_bind    309..323
                     /citation=[1]
                     /site="PR4"
                     /evidence=DNaseI footprinting
                     /note="CCAAT box"
     protein_bind    326..337
                     /citation=[1]
                     /site="PR3"
                     /evidence=DNaseI footprinting
                     /note="E2F"
     protein_bind    348..400
                     /citation=[1]
                     /site="PR2"
                     /evidence=DNaseI footprinting
                     /note="E2F and two E-boxes"
     protein_bind    408..427
                     /citation=[1]
                     /site="PR1"
                     /evidence=DNaseI footprinting
                     /note="TATA"
     protein_bind    435..477
                     /citation=[1]
                     /site="PR0"
                     /evidence=DNaseI footprinting
                     /note="E-box and transcription start"
     TATA_signal     414..417
ORIGIN
        1 tttgttttaa aaatatgcat acaatttaaa tatgttatta caccggtcgc tttggcactg
       61 ctgaaaaagt actttaattt cttttctctc tctcattgta tggatcaact atgtgactaa
      121 tttacattcc tagctacatg tatagcacca gcaggtgttt gacgagtgga tacctccatc
      181 gcgcatacca ccattgaatg ttaccactcc gactcatcaa ggcaagagga acgacctcta
      241 ttgatcgcct agcaatgcat cttttacatc accattgacc aatcagcgcc gtgaagtagc
      301 gatcaacgga ccaatcaggt tgcccccgcg atattcaagc acggtccgcg acaataacaa
      361 aaatcctacg tgcattatcc atatgcgttc attcatttgc acacactgca ctatatattg
      421 cgttagctgc ctacacattt gaatccgact cattctgaaa gctgaaaaca actcggacag
      481 ttctcatcct agacatcgat aatcgaagaa tttccatact ttgnagtgct aaatcatg