Gene Networks Database

LOCUS       PR_SPMSP130   867 bp    DNA             INV       06-MAY-1998
DEFINITION  Strongylocentrotus purpuratus msp130 gene promoter structure
DBSOURCE    GENBANK:  name SUSMSP130A, accession M31750
KEYWORDS    cell surface glycoprotein; mesenchyme-specific cell surface
            glycoprotein.
SOURCE      S.purpuratus sperm DNA, clone HG 2A.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  
  AUTHORS   Parr,B.A., Parks,A.L. and Raff,R.A.
  TITLE     Promoter structure and protein sequence of msp130, a lipid-anchored
            sea urchin glycoprotein
  JOURNAL   J. Biol. Chem. 265, 1408-1413 (1990)
  MEDLINE   90110195
COMMENTS    The promoter region of msp130 contains such putative regulatory
            elements:  possible TATA box (TATTA), possible CCAAT box (CCAT), 
            CGCTCAT heptamers (and variants with base mismatch), and GCCCCT 
            hexamer. Without additional data it is impossible to define promoter
            elements that regulate primary mesenchyme cell gene expression, 
            but some sequence elements, the CGCTCAT and GCCCCT motifs 
            (which also occur in the promoter region of SM50 gene and has been
            identified as possible regulatory elements) serve as examples 
            with possible functional significance. 		
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
  TATA_signal        640..644
                     /evidence=sequence inspection
                     /citation=[1]   
  misc_feature       438..441, 462..465, 535..538, 550..553, 565..568
                     /note="CCAAT sequence (CCAT-like box)"
                     /evidence=sequence inspection
                     /citation=[1]
  misc_feature       654..660, 735..741
                     /note="CGCTCAT_motif"
                     /evidence=sequence inspection
                     /evidence=
                     /citation=[1]
  misc_feature       432..437
                     /note="GCCCCT_motif"
                     /evidence=sequence inspection
                     /citation=[1]
  prim_transcript    668..
                     /note="cell surface glycoprotein (msp130) mRNA"
                     /evidence=nuclease S1 protection assays

ORIGIN     
        1 gccataagac gaaagacttc atgcatggct tattatcaac gaaacggagt taagtctttt
       61 gttaattctg cttgttgcta cggaaggcag ccagtaaaaa cgaattcgtt taatgaactt
      121 atggaaatgc aagccttact ttaaacaaaa agaaatttgt gacaactagt gtagataatg
      181 attaaaaggg ggttatagct agtctgtaaa aatgttccga caaccagtaa cagtgaagcc
      241 gttcaaaaac atcaaacaaa tctatccccc cccccacaaa aaaaacaaac aaaaaacttc
      301 aggttataag agacctctaa attttaatat tgtgtgataa gtgtaaaggt tttctaatat
      361 ccacaatttc tttaataaag attaattatc tttcgaggac taaaatggag atgtcatgac
      421 attttagcga tgcccctcca tatatcttgt cttagatggt tccataactt tggtctgtat
      481 ttcgcgacta ttctttaccc ccttcaagct ctgctttgcg agttcctgtt tataccatat
      541 ttcttctatc cattacccgg gagaccattt attctcacct ctacattcgg ctgttgtgaa
      601 cgaggcaccg actcgatctg tgcgagacaa ggctagacct attaaagagg atccgctctt
      661 ttttctttta ccagaagttg aaaagttttc gcttgaagcg caccacgaaa actctgccgg
      721 tagctgcaga gcaacgctca ttctggtgga aagagtacct ttggacaaag caagaagagc
      781 ggagttccac agctctttgg gagtactttt ttgattccca gaatacttgg gagaatttat
      841 ttttacggtc tctaatttga caaaatg