Gene Networks Database

LOCUS       Pr_SPPM27     1209 bp    DNA             INV       06-MAY-1998
DEFINITION  Strongylocentrotus purpuratus PM27 gene, promoter region
DBSOURCE    GENBANK:  name  SPU18132, accession U18132
KEYWORDS    .
SOURCE      Sea urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1
AUTHORS     Harkey,M.A., Klueg,K., Sheppard,P. and Raff,R.A.
TITLE       Structure, expression, and extracellular targeting of PM27, a
            skeletal protein associated specifically with growth of the sea
            urchin larval spicule
JOURNAL     Dev. Biol. 168 (2), 549-566 (1995)
MEDLINE     95246928
REFERENCE   2
AUTHORS     Raman,V., Andrews,M.E., Harkey,M.A. and Raff,R.A.
TITLE       Protein-DNA interactions at putative regulatory regions 
            of two coordinately expressed genes, msp130 and PM27, during
            skeletogenesis in sea urchin embryos.
JOURNAL     Int. J. Dev. Biol. 37, 499-507 (1993)
MEDLINE     87191419  
COMMENTS    The promoter region of the PM27 gene contains neither a
            TATA motif at 25-30 bases upstream of the transcription
            start site nor a CCAAT motif. Instead, as in case for the
            SM50 gene, and for the ectoderm-specific Spec2c gene,
            this region is GC-rich. At least four sequence motifs in
            the PM27 promoter (two P3A sites, a P3B site and a P71 site)
            are identical to protein binding sites of CyIIIa actin
            and other genes of S. purpuratus. Comparison of the
            PM27 promoter to SM50 promoter and 850-bp region of msp130 gene 
            located between exons 6 and 7 revealed three larger sequence
            elements (designated A, B and C) that are strictly similar
            in these genes. A and B elements bind proteins on gastrula 
            and pluteus stages, while no binding was observed with morula 
            extracts. Competitive gel shift assay showed that A and B 
            elements of PM27 and msp130 genes bind the same proteins.
            The A and C elements identified in this work seem to be not identical to A and C
            elements in the SM50 regulatory region, which are
            identified by Makabe et al (1995).
FEATURES            Location/Qualifiers
     source          1..1209
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /clone="SpMG20-Xba-A"
     mRNA            1081..
    element A        149..173
                     /evidence=sequence inspection
                     /evidence=DNase I footprinting
                     /gel shift assays
                     /citation=[1,2]
    element B        181..197
                     /evidence=sequence inspection
                     /evidence=DNase I footprinting
                     /evidence=gel shift assays
                     /citation=[1]
    element C        601..614
                     /evidence=sequence inspection
                     /citation=[1]
    misc_feature     361..369
                     /site="P7I"
                     /evidence=sequence inspection
                     /citation=[1]
    misc_feature     963..970
                     /site="P3A"
                     /evidence=sequence inspection
                     /citation=[1]
    misc_feature     979..1007
                     /site="P3B"
                     /evidence=sequence inspection
                     /citation=[1]
    misc_feature     1064..1071
                     /note="P3A"
                     /evidence=sequence inspection
                     /citation=[1]
ORIGIN
        1 ctgcaggcga ctctagaaat gattgagaat gctgaataaa catgatttct attatggtat 
       61 tttagaatca tcaaaacgaa acgatgtttc ctttgtaaag caatgtacac acaaatatga
      121 ctacatggac tacgtccact cactcactca ctcatataca cattcacgta catagcagca
      181 aaagttggtc caaagtttta aatatttctc gttgaattat aaagcaatat ttgacttcaa
      241 atcaattagc aactctcgta ctcttgtaaa cataaaagtt ctacagttca cttttttatt
      301 gaattaagaa ctctaatata tatggaatgt gagggtataa caagagcaat gttttttcgt
      361 tgttcttatt aggggggggg ggttgtgagt gtgtgagacc tcattccata gagaggaaaa
      421 aatggttatc ccgaaaatca ttttctcctc cacaagatct aaacttcttg tttaacatat
      481 taacatgtat gcagtggaat aatatgttga aaatagaaga gactaaaata attcaattgg
      541 aaagacgatt tgaaacttat tctatgtttt tacaaggtta aacttactgg aaacatttcc
      601 gttttgaggt gcggtatcat gaacgggaaa catgtttagg gaaaaagatc tattaatttt
      661 ctgaattttc tgaagatgac ccttcaccta aataacaact tacttttctt taaatacact
      721 ttcacatctt tttgaattat atggggcctc ctttttttcg ttctatcaaa atgtgttata
      781 tacgcaaaat aaagtttggt cagagtacag gattaggagg cagattagga gttaaggcaa
      841 aaacagggtt ggatatatat tattatgtgg atgtacgcac atgtcgccat attaactctt
      901 gccgctaact tgtccccttt gggtatttcc tgctgcgtat tctggtccaa aattagtcac
      961 tctttgcgca tttccttcat ttgcatacga tccacccctt ttaggagagg ggcgtggcga
     1021 ctgctgcttc cgtgccgtgt gcaaacatcc ccctgacccc ttcagcgcac gtttatgtgc
     1081 attcgaagcc aggcagctca cttcagtaag tcattctgtg aattcactct tactgttgaa
     1141 cattgtgcag ggttatacag ccagatactt gcctgaaagg aaaacaacag agaactttac
     1201 ttagccatg