Gene Networks Database

LOCUS       Rr_SPU6      338 bp    DNA             INV       27-MAY-1998
DEFINITION  SpU6 gene regulatory region
DBSOURCE    GENBANK:  name S82534, accession X76546
KEYWORDS    U6 small nuclear RNA; U6 snRNA; U6.2 snRNA gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Marzluff,W.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-1993) W.F. Marzluff, Univ. of North Carolina at
            Chapel Hill, Program in Molecular Biology, CB#7100, Chapel Hill, NC
            27599, USA
REFERENCE   2  (bases 1 to 335)
  AUTHORS   Sakallah,S.A., Norton,D.R., Zhang,W. and Marzluff,W.F.
  TITLE     Isolation and characterization of the U6 snRNA genes from the sea
            urchin Strongyocentrotus purpuratus
  JOURNAL   Unpublished
REFERENCE   3 (sites)
  AUTHORS   Li, J.-M., Parsons, R. A. and Marzluff, W. F. (1994).
  TITLE     Transcription of the sea urchin U6 gene in vitro requires
            a TATA-like box, a proximal sequence element, and sea urchin
            USF, which binds an essential E box.
  JOURNAL   Mol. Cell. Biol. 14 (3): 2191-2200
  MEDLINE   94325356
COMMENTS    Only 88 nucleotides of 5` flanking sequence of the U6 gene are required
            for the maximal expression in vitro. Mutagenesis experiments demonstrated
            the requirement for three elements, a CACGTG element (E-box) at -80 (199-204
            of this entry), a proximal sequence element at about -55 (217-236) and the
            TATA-like box (TTAAA) at -25 (251 of this entry). The TTAAA sequence is
            important but not essential for tanscription, but the E-box is the essential 
            element. The major protein in the sea urchin extracts that interacts with
            the CACGTG element is sea urchin USF
            (SpF1) and immunodepletion of it greatly reduces transcription. The USF
            binding site in the U6 gene is highly homologous (11 0f 13 nucleotides)
            with the USF binding sites found in the promoter of the S. purpuratus 
            Spec genes [3] 
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /germline
                     /cell_type="sperm"
     protein_bind    199..204
                     /bound_moiety="USF"
                     /site="E-box"
                     /evidence=mobility shift assays                     
                     /citation=[3]    
     misc_feature    215..236
                     /note="proximal sequence element"
                     /evidence=series of scanning mutations                     
                     /citation=[3] 
     misc_feature    251..255
                     /note="TTAAA sequence (TATA-like box)"
                     /evidence=series of scanning mutations                     
                     /citation=[3] 
     misc_RNA        281..
                     /gene="U6 snRNA"
                     /product="U6 small nuclear RNA"
     gene            281..
                     /gene="U6 snRNA"
ORIGIN      
        1 tttggacttt gcacaaagga agcatccgtg cgtgcaaatg cctcgtgggc gttttgtata
       61 cacaaagatt gtgtgcgctc cttattggtt ggattaaggg tcacacacga tcattctttt
      121 atgtggtcca gtgttattca aatcagtggt aaatagaacc gttcagaact cattgcaaaa
      181 ctccactgcg tacgggaaca cgtgcttacg gcgcaaaact gtgcggaatt gaagtttatg
      241 ggcggattgt ttaaagggag tgctgctctc aggtactttc gtgttagctt cggcaacaca
      301 tctattaaaa ttggaacgat acagagaaga ttagcatg