LOCUS       SPSPE2A1      539 bp    DNA             INV       14-FEB-1996
DEFINITION  S.purpuratus Spec2a gene 5'-flank and exon 1 (and joined CDS).
ACCESSION   X12771
NID         g10285
KEYWORDS    calcium binding protein; Spec2 gene.
SOURCE      purple urchin.
  ORGANISM  Strongylocentrotus purpuratus
            Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
            Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
            Strongylocentrotidae; Strongylocentrotus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Hardin,P.E., Angerer,L.M., Hardin,S.H., Angerer,R.C. and Klein,W.H.
  TITLE     Spec2 genes of Strongylocentrotus purpuratus. Structure and
            differential expression in embryonic aboral ectoderm cells
  JOURNAL   J. Mol. Biol. 202 (3), 417-431 (1988)
  MEDLINE   89011969
COMMENT     see x12771, x14529 - x14533 for Spec2a gene exon seq Data kindly
            reviewed (5/589)) by Klein W.H.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Strongylocentrotus purpuratus"
                     /db_xref="taxon:7668"
                     /clone_lib="lambda EMBL3"
     TATA_signal     385..391
     exon            415..533
                     /gene="Spec2a"
                     /label=ex1
     mRNA            join(415..533,X14529:15..51,X14530:14..157,X14531:15..135,
                     X14532:15..133,X14533:2..174)
                     /gene="Spec2a"
                     /label=SPE_mRNA
     misc_feature    531..533
                     /gene="Spec2a"
                     /label=start
     CDS             join(531..533,X14529:15..51,X14530:14..157,X14531:15..135,
                     X14532:15..133,X14533:2..30)
                     /gene="Spec2a"
                     /function="calcuim binding protein"
                     /codon_start=1
                     /label=SPE_CDS
                     /db_xref="PID:e222193"
                     /db_xref="PID:g1197382"
                     /db_xref="SWISS-PROT:P04110"
                     /translation="MAVQLLFTEEEKALFKSSFKSEDTDGDGKITSEELRAAFKSIEI
                     DLTQEKIDEMMGMVDKDGSKDMDFSEFLMRKAEQWRGREVQLTKAFVDLDKDHNGSLS
                     PQELRTAMSACTDPPMTEKEIDAIIEKADCNGDGKICLEEFMKLIHSS"
BASE COUNT      157 a    127 c     92 g    162 t      1 others
ORIGIN
        1 gtaacggata aaacaacaaa aagtccacag gagtggttgt tagaagctga aactacatac
       61 aactttcatt ctctcctaac gaaggacgat tttntttaac agcgggtttt cttggacacc
      121 aattttttct ttattaatga ggtccaaggg tccgccttag gtcccctggt agccattgtg
      181 agcgataaca actctagttc atcccttgag attttaatcg cactgccccc attgtgcgct
      241 cctttcagag aggcacaaac acccctcaga tttaacattc tatctcactc aaaaatagca
      301 gacacgctct taactaaaac aacaacaaca acattcctag catatggtca cgtgctttat
      361 ttcaggagtt atcactcttt cttgaataat actcttcgtc agcactccac ccttgccact
      421 ccttgaaagc aagtcgacta gaagagaggt ttagacttta ggtacctgag atccagagaa
      481 atttttactt tctatcttca attttggccg tgtcaatcca atttttaagg atggtaagt
//