LOCUS SUSHISES3 2424 bp DNA INV 18-MAR-1994
DEFINITION Sea urchin (S.purpuratus) early histone genes H2B ( H2B alpha )
and H3.
ACCESSION J01173 J01205 M10375 V01356
NID g161503
KEYWORDS histone H2B; histone H3.
3 of 4
SOURCE Sea urchin (S.purpuratus) DNA, clones pSp[2,17]; clone pSp2b-1 [4].
ORGANISM Strongylocentrotus purpuratus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Echinodermata;
Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida;
Strongylocentrotidae; Strongylocentrotus.
REFERENCE 1 (bases 1868 to 1953)
AUTHORS Sures,I., Maxam,A., Cohn,R.H. and Kedes,L.H.
TITLE Identification and location of the histone H2A and H3 genes by
sequence analysis of sea urchin (S.purpuratus) DNA cloned in E.
coli
JOURNAL Cell 9, 495-502 (1976)
MEDLINE 77089442
REFERENCE 2 (bases 392 to 2424)
AUTHORS Sures,I., Lowry,J. and Kedes,L.H.
TITLE The DNA sequence of sea urchin (s. purpuratus) H2A, H2B and H3
histone coding and spacer regions
JOURNAL Cell 15, 1033-1044 (1978)
MEDLINE 79084134
REFERENCE 3 (bases 515 to 648)
AUTHORS Levy,S., Sures,I. and Kedes,L.H.
TITLE Sequence of the 5'-end of Strongylocentrotus purpuratus H2b histone
mRNA and its location within histone DNA
JOURNAL Nature 279, 737-739 (1979)
MEDLINE 79199774
REFERENCE 4 (bases 1 to 636)
AUTHORS Mauron,A., Levy,S., Childs,G. and Kedes,L.
TITLE Monocistronic transcription is the physiological mechanism of sea
urchin embryonic histone gene expression
JOURNAL Mol. Cell. Biol. 1 (7), 661-671 (1981)
MEDLINE 97425329
COMMENT [3] mRNA start site.
All four segments of this entry are from a pair of clones pSp2 and
pSp17 which cover the early histone gene repeat. This unit is about
6.5 kb long, contains the H1, H4, H2b, H3 and H2A genes in that
order, and is repeated several hundred times. The five coding
regions are separated by DNA spacers not represented in polysomal
mRNA; there are no reported introns.
FEATURES Location/Qualifiers
source 1..2424
/organism="Strongylocentrotus purpuratus"
/db_xref="taxon:7668"
mRNA 536..>1008
/note="H2b mRNA [3]"
CDS 634..1008
/codon_start=1
/product="histone H2B"
/db_xref="PID:g161508"
/translation="MAPTAQVAKKGSKKAVKGTKTAXGGKKRNRKRKESYGIYIYKVL
KQVHPDTGISSRAMVIMNSXVXDIFERIAGESSRLAQYNKKXTXSSREIQTAVRLILP
GELAKHAVSEGTKAVTKYTTSK"
CDS 1787..2197
/codon_start=1
/product="histone H3"
/db_xref="PID:g161509"
/translation="MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRP
GTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTELRFQSSAVMALQEASEAYLV
GXXXDTNLCAIHAKRVTIMPKDIQLARRIRGERA"
BASE COUNT 747 a 578 c 477 g 575 t 47 others
ORIGIN About 300 bp after segment 2.
1 gcgcctttag atatattata taggttccct ttttcatttg ctgtaaccaa ataattttga
61 aatacaatgt caaagatatt tcattgctca tttctgttat atcattacgt ttattattat
121 tgtctatcat tgtgtatatt gtgtgagaag aaatgaaaaa taaattcgct ctttacatat
181 agagagagct actactacta ctatataatt atatatctcc gagataatgt caaaagtagg
241 agtgannnnn nnnnnnnnnn tgcaagtatg tcttggataa aaagtctcga catgttccat
301 attcccatca aaattcatcg tcttcttcaa ctttttcaca tttctcattc tttggggatt
361 gaattgaaca atgcagacag annnnnnnnn ntgcccgtat gatcactatg tcgccatctc
421 taggcagggg atggaacagg cactaanctg cgacgcctaa gaccaatgaa aggatcgaga
481 ccgaggctca tttgcatacg gaccgcagca tacggatccg gccccgtgta taaaaaggaa
541 aggttctcgc tggccattca cagtatccaa agaatatttg cttgacatac tcgtttcgct
601 gcatctttac agaccagaaa acctcaattc atcatggctc caacagctca agttgctaag
661 aaaggctcca agaaggcagt caaaggcacc aagacggccn gcggtggcaa gaagaggaac
721 aggaaaagga aggagagtta tggaatctac atctacaaag tcctcaagca ggttcatcca
781 gataccggca tctccagtcg ggccatggtc atcatgaaca gctnngtnna cgacatcttc
841 gagcgaattg ccggcgaatc ttcccgcctc gctcagtaca acaaaaagtn nacnntcagc
901 agtcgcgaga ttcagaccgc cgtccgcctc attctccccg gagagctggc aaagcacgct
961 gtgagcgagg gtaccaaggc agtgacgaaa tacactacct ccaagtagac aggtcatatc
1021 ctgctctaat tggacataat acaacggccn nacaaataat caagaaagaa tgatatccgt
1081 agtaatgtag cgtagttgtt tattaaataa ataatgttgt ataacgaata ttaagatata
1141 atcaagatta cacaatagta tgtaataata ttagccctat ataaatctca ctctcataaa
1201 tctagactag agcatgaagt gtagtcatga tggtcggaag gaagacgaaa atagttgatt
1261 aattaaataa aaagaaatac aattcgagat ggcaacatgt cctaaatgat atacgtccat
1321 gcatcctttc cttccttgaa ngtnnacaga tgcaggttaa gtcttttctc atgattcatc
1381 accacccagc tccctgcctg cctccctctc atccgccctc ttaagatcag ccgagcagac
1441 tctcggtcaa acaccttaat catcatttgt tagaatgcat attttcgtaa ttatttggca
1501 ccactgagtg cgtgagccga attcccggct tgccatgtaa cccatgttgc aaacggtggt
1561 agcgccacct ttcgacacat tttattgact ttgcacatac ggcatcgcca agcccccttc
1621 ccgtcacgcg ctaaacaaaa gagcaacccg gttgaccaat caagagagct ttacaaacgg
1681 caccaggatc ccgcagcaca tataaatagc tgaaaattgc cagtggttct cattcatccc
1741 gtcactcgta tttgaagtac tgaattacct actgtcccaa gcaactatgg cacgcaccaa
1801 gcagaccgct cgcaaatcta caggagggaa ggctccccgc aagcagctgg caaccaaagc
1861 tgccagaaag agtgcccccg ccactggagg agtcaagaag cctcatcgat acaggcctgg
1921 cacagtcgcc ctgagagaga ttcgccgcta ccagaagagc actgagcttc tcatccgaaa
1981 actgccattc cagcgtctag tgcgtgagat tgcacaggac ttcaagacag agctacgttt
2041 ccagagttcc gctgtgatgg cccttcaaga agccagcgag gcatacctag ttggccnnnn
2101 nngagacacc aacctgtgtg ccatccacgc caagagggtt accatcatgc ccaaagacat
2161 ccagctcgcc cgtcgaatcc gcggagaacg cgcctagaac catcggtaca gcatgtagcc
2221 catgcaccgc atacacaaac ggctcttttc agagccacca caaccccaag aaagantcat
2281 tgatttctaa aatgaacacg atgcatgaca atcgacgcta ttataagaag aaagaaaaaa
2341 acaacaacaa acatttgtag atcataattg agatgaacga tatgatgaaa tgtagaacat
2401 aatttagata cacgatatga taaa
//