Click to see a larger version of this image.

Click to See Photos of Workshop

Only For Faculty at American Institutions

2008 Workshop #1 (20 participants)

2008 Workshop #2 (20 participants)

2008 Instructors

Todd Eckdahl, Laurie Heyer, Anne Rosenwald, Consuelo Alvarez,
Charles Hauser, Malcolm Campbell, Edison Fowlks

Travel and Contact

Below please find some links for directions and a campus map—(on the map it is building H for Humanities/ Social Sciences, note location of McCarthy Hall [MH] and Dan Black Hall [DBH] where the computer room [MH501] and experimental labs [DBH 104 and 161] for the workshop are located). Let me know about the number of parking passes you will need. Looking forward to the workshop!

Highlights:

Free Software and Training

Interdisciplinary Team Participation Encouraged

Produce RNA; cDNA probes; Hybe; Wash; Scan; Analyze

Yeast full-genome microarrays

Computers Provided but You Can Bring Laptops

The first change is the organization of the days, as seen above. Part of this schedule adjustment is required because of the second major change, the use of Genisphere’s 3DNA method instead of direct incorporation. Finally, we will no longer rely on burning CDs and will purchase GCAT Flash Drives (1 – 2 GB each) for the participants (with space for initials). This will allow us to deliver the workshop materials and for them to archive all their data. We will collect all the drives during the scanning period and return them with all the tiff files the next morning in time for dry lab.

Workshop #2 will spend the afternoon of Day 2 in the wet lab. At this time, they will freeze their cDNA. At the end of Day 3, workshop #2 will spend about 1.5 hours in the wet lab starting the overnight hybe. At this same time, workshop #1 will be finished in the lab and they will move to a classroom for curriculum discussions.

Another major change is that participants will prep their own RNA. We will provide for them frozen yeast pellets and the RNA isolation kits. They will analyze the quality of their RNA by spectrophotometer only (i.e., will not run gels) and decide if they want to use their RNA or some prepared by the instructors prior to the workshop. Participant RNA isolation will take place on Day 2 for both workshops during their half day of wet lab.

Selection Criteria

GCAT Protocols

Additional Online Resources

Purpose: Genome Consortium for Active Teaching (GCAT) offers hands-on workshops for faculty who conduct research with undergraduate students and/or teach undergraduate courses to learn about gene expression analysis via microarrays.

Workshop Organization:
Part 1 of the workshop begins with data analysis (see schedule below) to introduce the microarray method and will cover data analysis using public domain data from the literature and from GCAT. Participants will learn public domain and open source MAGIC Tool spot-finding and analysis software. The MIAME background information and its use in microarray evaluation will be covered. Participants will analyze data sets in short projects. Each of the two workshops has room for 20 participants.

Part 2 will be a hybridization workshop which will involve hands-on preparation of fluorescently labeled probes for yeast expression microarrays, their hybridization, data acquisition and data analysis using the methods presented in part 1 of the workshop.

Part 3 concludes with data analysis of the microarrays you produced. This will also serve to reinforce what you learned about data analysis.

Where: California State University, Fullerton

When: workshop #1: July 14 - 18; and workshop #2: 15 - 19, 2008

Costs and Scholarships: If funded by the NSF, this workshop will be completely free (housing, food, lab fees) for each participant with one catch. You pay for your airfare in advance but you will be reimbursed if you submit an action plan for implementing microarrays in your curriculum and submit receipts. For those with financial need for airfare in advance, you may petition to have this paid in advance.

Only For Faculty at American Institutions

Email Edison Fowlks or Malcolm Campbell with Questions

See photos from 2003 workshops

See photos from 2004 workshops

See photos from 2005 workshops

See photos from 2007 workshops

GCAT Home Page

Biology Home Page