2007 GCAT Microarray Workshop
One Session; 20 people
Week of May 22 - 25: University of Toronto
Workshop Schedule
Instructors:
Malcolm Campbell, Todd Eckdahl, Laurie Heyer
Time |
Activity |
Lead Instructors |
| 9:00 - 10:00 | Greetings
and Overview (quick
links to short and long animations) Launch |
Malcolm Campbell Laurie Heyer |
| 10:00 - 10:30 | Making a Project |
Laurie Heyer |
| 10:30 - 10:45 | Short Break | Dr. Pleez B. Prompt |
| 10:45 - 12:15 | Slide Layout and Gene List Practice Layout and Gene List |
Laurie Heyer |
| 12:15 - 1:15 | Lunch Break | |
| 1:15 - 2:15 | Addressing and Gridding Practice Gridding |
Laurie Heyer |
| 2:15 - 3:15 | Segmentation Practice Segmentation |
Laurie Heyer |
| 3:15 - 3:30 | Short Break | Dr. B. Ohn Thyme |
| 3:30 - 5:00 | Generate Multiple Ratio Expression Files Practice Multiple Ratio Expression Files Quit MAGIC Tool |
Laurie Heyer |
Goal for Day 1: Create 6 Columns
of Data |
||
Day 2 (May 23)
Time |
Activity |
Lead Instructors |
| 9:00 - 10:00 | Log Transforming Data Gene Information Practice Gene Information and Log Transforming Data |
Laurie Heyer |
| 10:00 - 11:00 | Explore Data | Laurie Heyer |
| 11:00 - 11:15 | Short Break | Dr. Don T Linger |
| 11:15 - 12:00 | Practice Exploring | Laurie Heyer |
| 12:00 - 12:45 | Lunch Break | |
| 12:45 - 1:30 | Set up Reverse Transcription and Cy-Dye Incorporation | Todd Eckdahl |
| 1:30 - 2:00 | Wet Lab Overview | Todd Eckdahl |
| 2:00 - 2:45 | Discussion DeRisi Experiment; Explore DeRisi Data RNA Isolation & Quality Control |
Todd Eckdahl |
| 2:45 - 3:15 | Discussion of Labeling Alternatives | Todd Eckdahl |
| 3:15 - 3:30 | Short Break | Dr. R Ush Bach |
| 3:30 - 4:00 | RNA Degradation | Todd Eckdahl |
| 4:00 - 4:30 | Purification of cDNA | Todd Eckdahl |
| 4:30 - 5:00 | Prehybridization Procedures, & Measurement of Labeling Efficiency |
Todd Eckdahl |
| 5:00 - 5:30 | Labeled Sample Preparation and Hybridization |
Todd Eckdahl |
Goal for Day 2: Manipulate and Explore Data Generate cDNA Probes & Hybe Them on Chips |
||
Day 3 (May 24)
Time |
Activity |
Lead Instructors |
| 9:00 - 10:00 | Discussion of Quantifying mRNA Levels with RT PCR Discussion of Using Microarrays in Courses |
Todd Eckdahl Todd Eckdahl and Malcolm Campbell |
| 9:45 - 10:00 | Short Break |
|
| 10:00 - 11:00 | Posthybridization Washing | Todd Eckdahl |
| 11:00 - 12:45 | Microarray Slide Scanning | Todd Eckdahl |
| 12:45 - 1:30 | Lunch Break | |
| 1:30 - 2:30 | Discussion of Results | Instructors and Participants |
| 2:30 - 3:30 | Discussion of Using Microarrays in Research | Eckdahl, Heyer, and Campbell |
| 3:30 - 5:00 | Flexible Catch Up Time | GCAT Instructors |
Goal for Day 3: Wash & Scan Chips Discuss and Trouble Shoot Results |
||
Day 4 (May 25)
Time |
Activity |
Lead Instructors |
| 9:00 - 11:00 | Refresh Your Memory Generate Ratios from Your Microarray |
Laurie Heyer You |
| 11:00 - 11:15 | Short Break |
Dr. Hur E. Back |
| 11:15 - 1:00 | 1 Column Exploration Make a Series from Separate 1 Columns Clustering Overview (you practice later: online + PDF) |
Laurie Heyer |
| 1:00 - 2:00 | Lunch Break, Workshop Assessment, and Closing Discussions | You and Heyer, Eckdahl, Campbell |
Goal for Day 4: Know what to
do after wet lab! |
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See photos from 2003 workshops
See photos from 2004 workshops
© Copyright 2007 Department of Biology, Davidson College,
Davidson, NC 28035
Send comments, questions, and suggestions to: macampbell@davidson.edu
