Dr. Karmella Haynes and Samantha Simpson ('09) Continued Flipping
We constructed 2 stacks of one pancake
(promoter flavored)
It flips!!
We constructed 2 stacks of one pancake
(gene flavored)
It flips, too!!
We Built A Lot of Parts!
Unexpected Problems = opportunities to learn more
Position Matters for RBS
It Flips Too Fast!
Needed Two Plasmids to Reduce Hin Production; Remove RE
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Pancakes in pSB1A7 |
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Hin in pSB3K3 |
Read Through Transcription
Tetracycline Resistance Takes Very Few Molecules
Built Insulated Plasmid
Convert Constructs to Numerical Symbols
Biological Equivalents
(1) , (2) = (-2) , (-1)
Distinguish 1,2 from -2, -1
pBad is not good promoter
What Are We Working On Right Now?
Model predicts behavior of all 8 constructs.
Mouse over this figure.
pLac Promoter and Tetr Reveal Secrets
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White Colonies Live on Tet Plates |
Faint Pink Colonies Live on Tet
Plates |
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Cells Die |
Red Colonies Live on Tet Plates |
Cross-trained students in biology and math/CS
Understand: Promoters, Reporter Strengths, Critical Parts, and System Behavior
Near completion of biological computer
Produce better transgenic organisms?
Biological digital data storage (0's and 1's) device?
Massive computation as cheap as an undercooked hamburger!
