LOCUS       AF068759     3622 bp    DNA             BCT       01-MAR-2000
     DEFINITION  Lactococcus lactis subsp. lactis pyruvate carboxylase (pyc) gene,
                 complete cds.
     ACCESSION   AF068759
     VERSION     AF068759.1  GI:6455966
     KEYWORDS    .
     SOURCE      Lactococcus lactis subsp. lactis.
       ORGANISM  Lactococcus lactis subsp. lactis
                 Bacteria; Firmicutes; Bacillus/Clostridium group; Streptococcaceae;
                 Lactococcus.
     REFERENCE   1  (bases 1 to 3622)
       AUTHORS   Wang,H., O'Sullivan,D.J., Baldwin,K.A. and McKay,L.L.
       TITLE     Cloning, sequencing, and expression of the pyruvate carboxylase
                 gene in Lactococcus lactis subsp. lactis C2
       JOURNAL   Appl. Environ. Microbiol. 66, 1223-1227 (2000)
     REFERENCE   2  (bases 1 to 3622)
       AUTHORS   Wang,H., O'Sullivan,D.J. and McKay,L.L.
       TITLE     Direct Submission
       JOURNAL   Submitted (27-MAY-1998) Food Science and Nutrition, University of
                 Minnesota, 1334 Eckles Ave., St. Paul, MN 55108, USA
     FEATURES             Location/Qualifiers
          source          1..3622
                          /organism="Lactococcus lactis subsp. lactis"
                          /strain="C2"
                          /sub_species="lactis"
                          /db_xref="taxon:1360"
          gene            105..3518
                          /gene="pyc"
          CDS             105..3518
                          /gene="pyc"
                          /function="converts pyruvate and carbon dioxide to
                          oxaloacetate"
                          /note="pyruvate:carbon dioxide ligase"
                          /codon_start=1
                          /transl_table=11
                          /product="pyruvate carboxylase"
                          /protein_id="AAF09095.1"
                          /db_xref="GI:6455967"
                          /translation="MKKLLVANRGEIAVRVFRACNELGLSTVAVYAREDEYSVHRFKA
                          DESYLIGQGKKPIDAYLDIDDIIRVALESGADAIHPGYGLLSENLEFATKVRAAGLVF
                          VGPELHHLDIFGDKIKAKAAADEAQVPGIPGTNGAVDIDGALEFAQTYGYPVMIKAAL
                          GGGGRGMRVARNDAEMHDGYARAKSEAIGAFGSGEIYVEKYIENPKHIEVQILGDSHG
                          NIVHLHERDCSVQRRNQKVIEIAPAVGLSPEFRNEICEAAVKLCKNVGYVNAGTVEFL
                          VKDDKFYFIEVNPRVQVEHTITELITGVDIVQAQILIAQGKDLHTEIGIPAQAEIPLL
                          GSAIQCRITTEDPQNGFLPDTGKIDTYRSPGGFGIRLDVGNAYAGYEVTPYFDSLLVK
                          VCTFANEFSDSVRKMDRVLHEFRIRGVKTNIPFLINVIANENFTSGQATTTFIDNTPS
                          LFNFPRLRDRGTKTLHYLSMITVNGFPGIENTEKRHFEEPRQPLLNIEKKKTAKNILD
                          EQGADAVVEYVKNTKEVLLTDTTLRDAHQSLLATRLRLQDMKGIAQAIDQGLPELFSA
                          EMWGGATFDVAYRFLNESPWYRLRKLRKLMPNTMFQMLFRGSNAVGYQNYPDNVIEEF
                          IHVAAHEGIDVFRIFDSLNWLPQMEKSIQAVRDNGKIAEATICYTGDILDPSRPKYNI
                          QYYKDLAKELEATGAHILAVKDMAGLLKPQAAYRLISELKDTVDLPIHLHTHDTSGNG
                          IITYSGATQAGVDIIDVATASLAGGTSQPSMQSIYYALEHGPRHASINVKNAEQIDHY
                          WEDVRKYYAPFEAGITSPQTEVYMHEMPGGQYTNLKSQAAAVGLGHRFDEIKQMYRKV
                          NMMFGDIIKVTPSSKVVGDMALFMIQNELTEEDVYARGNELNFPESVVSFFRGDLGQP
                          VGGFPEELQKIIVKDKSVIMDRPGLHAEKVDFATVKADLEQKIGYEPGDHEVISYIMY
                          PQVFLDYQKMQREFGAVTLLDTPTFLHGMRLNEKIEVQIEKGKTLSIRLDEIGEPDLA
                          GNRVLFFNLNGQRREVVINDQSVQTQIVAKRKAETGNPNQIGATMPGSVLEILVKAGD
                          KVKKGQALMVTEAMKMETTIESPFDGEVIALHVVKGEAIQTQDLLIEID"
     BASE COUNT     1163 a    667 c    731 g   1061 t
     ORIGIN
             1 caaatgtcag tcgtttaaaa gtgtataaaa gatttttata atatatggaa gagtgaaagg
            61 aattaataat tacctttcca aacaagcctc attaaggagt caaaatgaaa aaactactcg
           121 tcgccaatcg tggagaaatc gccgttcgtg tctttcgtgc ctgtaatgaa ctcggacttt
           181 ctacagtagc cgtctatgca agagaagatg aatattccgt tcatcgcttt aaagcagatg
           241 aatcttacct tatcggtcaa ggtaaaaaac caattgatgc ttatttggat attgatgata
           301 ttattcgtgt tgctcttgaa tcaggagcag atgccattca tcccggttat ggtcttttat
           361 ctgaaaatct tgaatttgct acaaaagttc gagcagcagg attagttttt gtcggtcctg
           421 aacttcatca tttggatatt ttcggcgata aaatcaaagc aaaagccgca gctgatgaag
           481 ctcaagttcc cggaattccc ggaacaaatg gtgcagtaga tattgacgga gctcttgaat
           541 ttgctcaaac ttacggatat ccagtcatga ttaaggcagc attgggcggc ggcggtcgtg
           601 gaatgcgtgt tgcgcgtaat gacgctgaaa tgcacgacgg atatgctcgt gcgaaatcag
           661 aagctatcgg tgcctttggt tctggagaaa tctatgttga aaaatacatt gaaaatccta
           721 agcatattga agttcaaatt cttggggata gtcatggaaa tattgtccat ttgcacgaac
           781 gtgattgctc tgtccaacgc cgaaatcaaa aagtcattga aattgctcca gccgtaggac
           841 tctcaccaga gttccgtaat gaaatttgtg aagcagcagt taaactttgt aaaaatgttg
           901 gctatgttaa tgctgggacg gttgaatttt tagtcaaaga tgataagttc tactttatcg
           961 aagtcaaccc acgtgttcaa gttgaacaca caattaccga gcttattaca ggtgtagata
          1021 ttgttcaagc acaaattttg attgctcaag gcaaagattt acatacagaa attggtatcc
          1081 cggcacaagc tgaaatacca cttttgggct cagccattca atgtcgtatt actacagaag
          1141 acccgcaaaa tggcttcttg ccagatacag gtaaaatcga tacctaccgt tcaccaggtg
          1201 gtttcggcat tcgtttggac gttggaaatg cctatgctgg ttatgaagtg actccctatt
          1261 ttgactcgct tttagtaaaa gtttgtacct ttgctaatga atttagcgat agtgtacgta
          1321 aaatggatcg tgtgcttcat gaattccgta ttcgtggggt gaaaactaat attccatttt
          1381 tgattaatgt tattgccaat gaaaacttta cgagcggaca agcaacaaca acctttattg
          1441 acaatactcc aagtcttttc aatttcccac gcttacgtga ccgtggaaca aaaaccttac
          1501 actacttatc aatgattaca gtcaatggtt tcccagggat tgaaaataca gaaaaacgcc
          1561 attttgaaga acctcgtcaa cctctactta acattgaaaa gaaaaagaca gctaaaaata
          1621 tcttagatga acaaggggct gatgcggtag ttgaatatgt gaaaaataca aaagaagtat
          1681 tattgacaga tacaacttta cgtgatgctc accagtctct tcttgccact cgtttgcgtt
          1741 tgcaagatat gaaaggaatt gctcaagcca ttgaccaagg acttccagaa cttttctcag
          1801 ctgaaatgtg gggtggggca acctttgatg tcgcttatcg tttcttgaat gaatcgcctt
          1861 ggtatcgtct acgtaaatta cgtaaactca tgccaaatac catgttccaa atgcttttcc
          1921 gtggttcaaa tgcagttgga tatcaaaact atcctgataa tgtcattgaa gaatttatcc
          1981 acgtagctgc acatgaagga atcgatgtct ttcgtatctt tgatagcctc aactggttgc
          2041 cacaaatgga aaaatcaatc caagcagtgc gtgataatgg aaaaattgcc gaagcaacca
          2101 tttgttatac aggagatatc cttgacccaa gtcgaccaaa atataatatc caatactaca
          2161 aagatttggc aaaagagtta gaagctactg gggctcatat acttgccgtt aaagatatgg
          2221 cgggcttgtt gaaacctcaa gcggcatatc gcttgatttc agaattaaaa gatacggttg
          2281 acttaccaat tcacttgcat acacatgata cttcaggaaa tggtattatt acctattctg
          2341 gtgcaactca agcaggagta gatattattg atgtggcaac tgccagtctt gctggtggaa
          2401 cttctcaacc ttcaatgcaa tcaatttatt atgcccttga acatggtccc cgtcatgctt
          2461 caattaatgt gaaaaatgca gagcaaattg accattattg ggaagatgtg cgtaaatatt
          2521 atgcaccttt tgaggcagga attacgagcc cacaaactga agtttacatg catgaaatgc
          2581 ctggcggaca atatactaac ttgaaatctc aagcagcagc tgttggactt ggacatcgtt
          2641 ttgatgaaat caaacaaatg tatcgtaaag taaacatgat gtttggcgat atcattaaag
          2701 taactccttc atcaaaagta gttggtgata tggcactctt tatgattcaa aacgaattga
          2761 cagaagagga tgtctatgcg cgaggaaatg agcttaactt ccctgaatca gtagtctcat
          2821 tcttccgtgg tgatttagga cagcctgttg gaggtttccc agaagaacta caaaaaatta
          2881 ttgtaaaaga caaatcggtc attatggatc gtccaggatt acatgccgaa aaagttgatt
          2941 ttgcaactgt aaaagctgac ttggaacaaa aaattggtta tgaaccaggt gatcatgaag
          3001 ttatctctta cattatgtat ccacaagttt tccttgatta tcaaaaaatg caaagagaat
          3061 ttggagctgt cacactactc gatactccaa ctttcttaca cggaatgcgc ctcaatgaaa
          3121 aaattgaagt ccaaattgaa aaaggtaaaa cgctcagcat tcgtttagat gaaataggag
          3181 aacctgacct cgctggaaat cgtgtgctct tctttaactt gaacggtcag cgtcgtgaag
          3241 ttgttattaa tgaccaatcc gttcaaactc aaattgtagc taaacgtaag gccgaaacag
          3301 gtaatccaaa ccaaattgga gcaactatgc ccggttctgt tcttgaaatc ctagttaaag
          3361 ctggagataa agttaaaaaa ggacaagctt tgatggttac tgaagccatg aagatggaaa
          3421 cgaccattga gtcaccattt gatggagagg ttattgccct tcatgttgtc aaaggtgaag
          3481 ccattcaaac acaagactta ttgattgaaa ttgactaact ttaaattaac cctaccatta
          3541 tggtaggatt ttttattttc taaaaaaaag taattttaat tttcagaaaa tatataattt
          3601 tcggaataaa aagtgtataa ta

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