LOCUS       SCPYC2G      5704 bp    DNA             PLN       18-NOV-1991
     DEFINITION  S.cerivisiae PYC2 gene for pyruvate carboxylase.
     ACCESSION   X59890
     VERSION     X59890.1  GI:4254
     KEYWORDS    PYC2 gene; pyruvate carboxylase.
     SOURCE      baker's yeast.
       ORGANISM  Saccharomyces cerevisiae
                 Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales;
                 Saccharomycetaceae; Saccharomyces.
     REFERENCE   1  (bases 1 to 5704)
       AUTHORS   Stucka,R.
       TITLE     Direct Submission
       JOURNAL   Submitted (31-MAY-1991) R. Stucka, Inst fuer Physiol Chemie,
                 Schillerstr. 44, W-8000 Muenchen 2, FRG
     REFERENCE   2  (bases 1 to 5704)
       AUTHORS   Stucka,R., Dequin,S., Salmon,J.M. and Gancedo,C.
       TITLE     DNA sequences in chromosomes II and VII code for pyruvate
                 carboxylase isoenzymes in Saccharomyces cerevisiae: analysis of
                 pyruvate carboxylase-deficient strains
       JOURNAL   Mol. Gen. Genet. 229 (2), 307-315 (1991)
       MEDLINE   92017667
     FEATURES             Location/Qualifiers
          source          1..5704
                          /organism="Saccharomyces cerevisiae"
                          /strain="C836"
                          /db_xref="taxon:4932"
                          /chromosome="II, right arm"
                          /map="15kb centromere distal from tRNA(Glu3) gene"
                          /clone="c411"
                          /clone_lib="cosmid library strain C836"
          protein_bind    1359..1371
                          /bound_moiety="ABF1 /GF1"
          protein_bind    1375..1384
                          /bound_moiety="GRF2"
          misc_feature    1448..1452
                          /note="CT-block"
          misc_feature    1455..1459
                          /note="CT-block"
          TATA_signal     1561..1566
          protein_bind    1573..1578
                          /bound_moiety="GCN4"
          TATA_signal     1786..1790
          gene            1851..5408
                          /gene="PYC2"
          CDS             1851..5408
                          /gene="PYC2"
                          /EC_number="6.4.1.1"
                          /codon_start=1
                          /product="pyruvate carboxylase"
                          /protein_id="CAA42544.1"
                          /db_xref="GI:4255"
                          /db_xref="SWISS-PROT:P32327"
                          /translation="MSSSKKLAGLRDNFCLLGEKNKILVANRGEIPIRIFRSAHELSM
                          RTIAIYSHEDRLSMHRLKADEAYVIGEEGQYTPVGAYLAMDEIIEIAKKHKVDFIHPG
                          YGFLSENSEFADKVVKAGITWIGPPAEVIESVGDKVSARHLAARANVPTVPGTPGPIE
                          TVQEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTC
                          FVERFLDKPKHIEVQLLADKHGNVVHLFERDCSVQRRHQKVVEVAPAKTFPREVRDAI
                          LTDAVKLAKVCGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVSAQI
                          QIAAGATLTQLGLLQDKITTRGFSIQCRITTEDPSKNFQPDTGRLEVYRSAGGNGVRL
                          DGGNAYAGATISPHYDSMLVKCSCSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTL
                          LTNPVFIEGTYWTTFIDDTPQLFQMVSSQNRAQKLLHYLADLAVNGSSIKGQIGLPKL
                          KSNPSVPHLHDAQGNVINVTKSAPPSGWRQVLLEKGPCEFAKQVRQFNGTLLMDTTWR
                          DAHQSLLATRVRTHDLATIAPTTAHALAGAFALECWGGATFDVAMRFLHEDPWERLRK
                          LRSLVPNIPFQMLLRGATGVAYSSLPDNAIDHFVKQAKDNGVDIFRVFDALNDLEQLK
                          VGVNAVKKAGGVVEATVCYSGDMLQPGKKYNLDYYLEVVEKIVQMGTHILGIKDMAGT
                          MKPAAAKLLIGSLRTRYPDLPIHVHSHDSASTRVASMTACALAGADVVDVAINSMSGL
                          TSQPSINALLASLEGNIDTGINVEHVRELDAYRAEMRLLYPCFEADLKGPDPEVYQHE
                          IPGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPTSKVVGDLAQFMVS
                          NKLTSDDIRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPLRSDVLRNKRRKLTCRPGL
                          ELEPFDLEKIREDLQNRFGDIDECDVASNNMYPRVYEDFQKIRETYGDLSVLPTKNFL
                          APAEPDEEIEVTIEQGKTLIIKLQAVGDLNKKTGQREVYFELNGELRKIRVADKSQNI
                          QSVAKPKADVHDTHQIGAPMAGVIIEVKVHKGSLVKKGESIAVLSAMKMEMVVSSPAD
                          GQVKDVFIRDGESVDASDLLVVLEEETLPPSPKKVIFTR"
     BASE COUNT     1659 a   1165 c   1226 g   1654 t
     ORIGIN
             1 gaattcctct gatttcgcca ataagtattt cagaaacatc ccactggatc acgggttcat
            61 tagtcttggt gggtacatga agttctcggg catgctttac attgtaataa ccatatatat
           121 catcttttgc accaaggaaa aaccctacgt agagtatttg cccaaagtgg agcccataaa
           181 tacaagtgac agagggtcaa agccgataag tattgagtat gacgacggtg atgtggtgtc
           241 aactcagaat acaagcagta taaagtacat ttaccgctgc tttataaaag tgttgaaatt
           301 gaagtccgta agaagcctag ccttcattca catgattttc gaaatttgcc tttcaatgca
           361 acgaagccgc cacaaacctg aaactactag agcaaggctt caaaagagaa gacttggctg
           421 tgacagtact catagacctg ccgttcgaaa tcatatttgg gtactatgtt gttaaatgga
           481 gctccgacaa ggaccccatg attcgtgaca atagaagatt aagaaacagc acgggcacca
           541 acaaggtcat caagttctta gttggggatg ccggcgtttt aacaccatgg ttatggggct
           601 tcttgttccg tctggcagcc gcagtcttgg gaagttacgt ggtgaagcaa tttcccaagg
           661 atggtgaaat atccacgggt tatttttgtc tcgtgatatc cagcacctct taggttcctt
           721 catgaatacc gtccagttca ttggaatatc ggccttccat acaagagttg cagaccccgt
           781 gctgggtggc acatatatga cactgttaaa taccctcagc aacttcggtg ggacatggcc
           841 gcggttaatc attatgtcca tgatcaacta cttcaccgtg tatagtgcac tattcctggc
           901 acaaataaag tatacgtaac tcacggcggc acgatgcaag cgtgcaccga gcttttgaat
           961 ggcaccgtga ccatcctgcg tgacggctat tacatcacca atctcatatg tattgtagtc
          1021 ggacttttcc tatattttgg atatttgaaa aggaaaatcc tccatttaca aagtctgcca
          1081 atcagttcct ggagatgtac gtaacgaccc ctcaaacaag aattgtacga cattacattc
          1141 aagaaaattt taacgaggta tcgtaatatt tacaaaatct ttttagctac tacaggaagc
          1201 agaaacaaaa tgagatatca ataccagtgt aaatctgtta atgagaatca tcacattatc
          1261 ccagtttttt ccatgcaagg gaaaaatata ctacacgaca tttctatact ttaattatca
          1321 attaaggtca tctttctgta cgaaaactaa ctacgattgt cactaacgac gtgtccctta
          1381 cccgacgcag ctgctgatag ctaccgcaca gcacagccag ctcttctgtg attggcagag
          1441 aggggtcctt cctgcggaag acgccgcaaa ggcacatatg actaaccttt ttttttcgca
          1501 attaattttc taatagtttt tcattttttt ttttacaatt ttggcagtta cgtctcgagc
          1561 tatttattat aagagtcaga attggcgcag ggagtgttaa gtaagaagta ctccccatcg
          1621 gatatttcct attgtgtttc tgtgattttt agtctttttt tcttttcttc tttctttcgg
          1681 tatccttact tgattacata cataaacaag cccctctttt cttccaaact cttgtagctt
          1741 actatcgtgg cccgtacatt tgagtttgat ttttttgcca attactatat tgcaaaataa
          1801 aggacagtta ctaggagaga aaataagaga catagagaac aaagtaaaat atgagcagta
          1861 gcaagaaatt ggccggtctt agggacaatt tctgtttgct cggcgaaaag aataagatct
          1921 tggtcgccaa tagaggtgaa attccgatta gaatttttag atctgctcat gagctgtcta
          1981 tgagaactat cgccatatac tcccacgagg accgtctttc aatgcatagg ttgaaggcgg
          2041 acgaagcgta tgttatcggg gaggagggcc agtatacacc tgtgggtgct tacttggcaa
          2101 tggacgagat catcgaaatt gcaaagaagc ataaggtgga tttcatccat ccaggttatg
          2161 ggttcttgtc tgaaaattcg gaatttgccg acaaagtagt gaaggccggt atcacttgga
          2221 ttggccctcc agctgaagtt attgaatctg tgggtgataa ggtctctgcc agacacttgg
          2281 cagcaagagc taacgttcct accgttcccg gtaccccagg acctatcgaa actgtgcaag
          2341 aggcacttga cttcgttaat gaatacggct acccggtgat cattaaggcc gcctttggtg
          2401 gtggtggtag aggtatgaga gtcgttagag aaggtgacga cgtggcagat gcctttcaac
          2461 gtgctacctc cgaagcccgt actgccttcg gtaatggtac ctgctttgtg gaaagattct
          2521 tggacaagcc aaagcatatt gaagttcaat tgttggctga taaacacgga aacgtggttc
          2581 atcttttcga aagagactgt tctgtgcaaa gaagacacca aaaagttgtc gaagtcgctc
          2641 cagcaaagac tttcccccgt gaagttcgtg acgctatttt gacagatgct gttaaattag
          2701 ctaaggtatg tggttacaga aacgcaggta ccgccgaatt cttggttgac aaccaaaaca
          2761 gacactattt cattgaaatt aatccaagaa ttcaagtgga gcataccatc actgaagaaa
          2821 tcaccggtat tgacattgtt tctgcccaaa tccagattgc cgcaggtgcc actttgactc
          2881 aactaggtct attacaggat aaaatcacca cccgtggttt ttccatccaa tgtcgtatta
          2941 ccactgaaga tccctctaag aatttccaac cggataccgg tcgcctggag gtctatcgtt
          3001 ctgccggtgg taatggtgtg agattggacg gtggtaacgc ttatgcaggt gctactatct
          3061 cgcctcacta cgactcaatg ctggtcaaat gttcatgctc tggttctact tatgaaatcg
          3121 tccgtaggaa gatgattcgt gccctgatcg aattcagaat cagaggtgtg aagaccaaca
          3181 ttcccttcct attgactctt ttgaccaatc cagtttttat tgagggtact tactggacga
          3241 cttttattga cgacacccca caactgttcc aaatggtatc atcacaaaac agagcgcaaa
          3301 aactgttaca ctatttggca gacttggcag ttaacggttc ttctattaag ggtcaaattg
          3361 gcttgccaaa actaaaatca aatccaagtg tcccccattt gcacgatgct cagggcaatg
          3421 tcatcaacgt tacaaagtct gcaccaccat ccggatggag acaagtgcta ctggaaaagg
          3481 gaccatgtga atttgccaag caagtcagac agttcaatgg tactctactg atggacacca
          3541 cctggagaga cgctcatcaa tctctacttg caacaagagt cagaacccac gatttggcta
          3601 caatcgctcc aacaaccgca catgcccttg caggggcttt cgctttagaa tgttggggtg
          3661 gtgcgacatt cgacgttgca atgagattct tgcatgagga tccatgggaa cgtctgagaa
          3721 aattaagatc tctggtgcct aatattccat tccaaatgtt attgcgtggt gccactggtg
          3781 tggcttactc ttcattacct gacaatgcta ttgaccattt tgtcaagcaa gccaaggata
          3841 atggtgttga tatatttaga gtctttgatg ccttgaatga tttagaacaa ttaaaagttg
          3901 gtgtgaatgc tgtcaagaag gccggtggtg ttgtcgaagc tactgtttgt tactctggtg
          3961 acatgcttca gccaggtaag aaatacaact tagattacta cctagaagtt gttgaaaaaa
          4021 tagttcaaat gggtacacat atcttgggta ttaaggatat ggcaggtact atgaaaccgg
          4081 ccgctgccaa attattaatt ggctccctaa gaaccagata tccggattta ccaattcatg
          4141 ttcacagtca tgactccgca agtactcgtg ttgcgtctat gactgcatgt gccctagcag
          4201 gtgctgatgt tgtcgatgta gctatcaatt caatgtcggg cttaacttcc caaccatcaa
          4261 ttaatgcact gttggcttca ttagaaggta acattgatac tgggattaac gttgagcatg
          4321 tccgtgaatt agatgcatat agggccgaaa tgagactgtt gtatccttgt ttcgaggccg
          4381 acttgaaggg cccagatcca gaagtttacc aacatgaaat cccaggtggt caattgacta
          4441 acttgttatt ccaagctcaa caactgggtc ttggtgaaca atgggctgaa actaaaagag
          4501 cttacagaga agccaattac ctactgggag atattgttaa agttacccca acttctaagg
          4561 ttgtcggtga tttagctcaa ttcatggttt ctaacaaact gacttccgac gatattagac
          4621 gtttagctaa ttctttggac tttcctgact ctgttatgga cttttttgaa ggtttaattg
          4681 gtcaaccata cggtgggttc ccagaaccat taagatctga tgtattgaga aacaagagaa
          4741 gaaagttgac gtgccgtcca ggtttagaat tagaaccatt tgatctcgaa aaaattagag
          4801 aagacttgca gaacagattc ggtgatattg atgaatgcga tgttgcttct aacaatatgt
          4861 atccaagggt ctatgaagat ttccaaaaga tcagagaaac atacggtgat ttatcagttc
          4921 taccaaccaa aaatttccta gcaccagcag aacctgatga agaaatcgaa gtcaccatcg
          4981 aacaaggtaa gactttgatt atcaaattgc aagctgttgg tgacttaaat aagaaaactg
          5041 ggcaaagaga agtgtatttt gaattgaacg gtgaattaag aaagatcaga gttgcagaca
          5101 agtcacaaaa catacaatct gttgctaaac caaaggctga tgtccacgat actcaccaaa
          5161 tcggtgcacc aatggctggt gttatcatag aagttaaagt acataaaggg tctttggtga
          5221 agaagggcga atcgattgct gttttgagtg ccatgaaaat ggaaatggtt gtctcttcac
          5281 cagcagatgg tcaagttaaa gatgttttca ttagggatgg tgaaagtgtt gacgcatcag
          5341 atttgttggt tgtcctagaa gaagaaaccc tacccccatc cccaaaaaaa gtaattttta
          5401 ctcgttaatt atattttatg acatctgaaa atactagctg tactatatat ggcgtatatt
          5461 ttatctagtt atgttccatg tatatttaaa tgccaaatag aaagtaatca aacactttcg
          5521 atgaaatacg tgctaactgt gtttcttcct taatgctttc acttaccatg tctccattct
          5581 ccattttctt cttgagtgaa aatgtgagtt tataacgctc aagtacgtta actactctat
          5641 ttaatatcgt acgggatttt tgatcgactg taggttttct tcttagacca ttccagcgcc
          5701 cggg

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