LOCUS
DMALD 9224 bp
DNA
INV 05-APR-1995
DEFINITION D.melanogaster ald gene for aldolase.
ACCESSION X60064
VERSION X60064.1 GI:7571
KEYWORDS ald gene; aldolase; fructose 1,6 biphosphate
aldolase.
SOURCE fruit fly.
ORGANISM Drosophila melanogaster
Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
Drosophilidae; Drosophila.
REFERENCE 1 (bases 1 to 9224)
AUTHORS Dorsett,D.
TITLE Direct Submission
JOURNAL Submitted (11-JUN-1991) D. Dorsett, Memorial Clean
Katering Cancer
Centre, 1275 York Ave., New York, NY 10021, USA
REFERENCE 2 (bases 1 to 9224)
AUTHORS Kim,J., Yim,J.J., Wang,S. and Dorsett,D.
TITLE Alternate use of divergent forms of an ancient
exon in the
fructose-1,6-bisphosphate aldolase gene of Drosophila melanogaster
JOURNAL Mol. Cell. Biol. 12 (2), 773-783 (1992)
MEDLINE 92123202
FEATURES
Location/Qualifiers
source 1..9224
/organism="Drosophila melanogaster"
/strain="Oregon R"
/db_xref="taxon:7227"
/chromosome="3, segment 97B, 3-91.5 m.u."
/clone="genomic clones: lambda G3,4,13,32; cDNA clones:
PSCE3,4,5"
/tissue_type="All and adult head"
/clone_lib="lambdaDASH Oregon R; lambdagt11 head cDNA Paul
Salvaterra; genomic Y.&L.Jan"
/dev_stage="All and adult head"
exon
1619..1692
/gene="ald"
/note="2.0 kb, 2.6 kb mRNAs"
/number=1
/evidence=experimental
mRNA
join(1619..1692,4983..5999,7506..8275)
/gene="ald"
/product="2.0 kb mRNA"
gene
1619..8865
/gene="ald"
mRNA
join(1619..1692,4983..5999,7506..8865)
/gene="ald"
/product="2.6 kb mRNA"
intron 1693..4982
/gene="ald"
/note="2.0 kb, 2.6 kb mRNAs"
/number=1
mRNA
join(3199..3227,4983..5999,6314..6402,6630..6928)
/gene="ald"
/product="1.6 kb mRNA"
exon
3199..3227
/gene="ald"
/note="1.6 kb mRNA"
/number=1
/evidence=experimental
intron 3228..4982
/gene="ald"
/note="1.6 kb mRNA"
/number=1
exon
4983..5999
/gene="ald"
/note="1.6 kb, 2.0 kb 2.6 kb mRNAs"
/number=2
/evidence=experimental
CDS
join(5004..5999,6314..6402,6630..6732)
/gene="ald"
/codon_start=1
/product="aldolase-related protein"
/protein_id="CAA42666.1"
/db_xref="GI:7572"
/db_xref="FLYBASE:FBgn0000064"
/db_xref="SWISS-PROT:P29841"
/translation="MTTYFNYPSKELQDELREIAQKIVAPGKGILAADESGPTMGKRL
QDIGVENTEDNRRAYRQLLFSTDPKLAENISGVILFHETLYQKADDGTPFAEILKKKG
IILGIKVDEGVVPLFGSEDEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPS
YQSILENANVLARYASICQSQRIVPIVEPEVLPDGDHDLDGAQKVTETVLAAVYKALS
DHHVYLEGTLLKPNMVTAGQSAKKNTPEEIALTTVQALRRTVPAAVTGVTFLSGGQSE
EEATVNLSAINNVPLIRPWALTFSYGRALQASVLRAWAGKKENIAAGQNELLKRAKAN
GEAACGNYTAGSVKGFAGKDTLHVDDHRPIPRPVRVFTCPAQFRRLPAMPTSLSPSTN
TKS"
CDS
join(5004..5999,7506..7595)
/gene="ald"
/EC_number="4.1.2.13"
/codon_start=1
/product="fructose-bisphosphate aldolase"
/protein_id="CAA42667.1"
/db_xref="GI:7573"
/db_xref="FLYBASE:FBgn0000064"
/db_xref="SWISS-PROT:P07764"
/translation="MTTYFNYPSKELQDELREIAQKIVAPGKGILAADESGPTMGKRL
QDIGVENTEDNRRAYRQLLFSTDPKLAENISGVILFHETLYQKADDGTPFAEILKKKG
IILGIKVDEGVVPLFGSEDEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPS
YQSILENANVLARYASICQSQRIVPIVEPEVLPDGDHDLDGAQKVTETVLAAVYKALS
DHHVYLEGTLLKPNMVTAGQSAKKNTPEEIALTTVQALRRTVPAAVTGVTFLSGGQSE
EEATVNLSAINNVPLIRPWALTFSYGRALQASVLRAWAGKKENIAAGQNELLKRAKAN
GDAAQGKYVAGSAGAGSGSLFVANHAY"
intron 6000..7505
/gene="ald"
/note="2.0 kb, 2.6 kb mRNAs"
/number=2
intron 6000..6313
/gene="ald"
/note="1.6 kb mRNA"
/number=2
exon
6314..6402
/gene="ald"
/note="1.6 kb mRNA"
/number=3
/evidence=experimental
intron 6403..6629
/gene="ald"
/note="1.6 kb mRNA"
/number=3
CDS
6630..6725
/partial
/gene="ald"
/note="mult aldolase-like peptide in -1 frame of 1.6 kb
mRNA, exon 4"
/codon_start=1
/protein_id="CAA42668.1"
/db_xref="GI:763087"
/translation="ANSQACQGIYVPGSIPSFAGNANLFVAQHKY"
exon
6630..6928
/gene="ald"
/note="1.6 kb mRNA"
/number=4
/evidence=experimental
polyA_signal 6907..6912
/gene="ald"
/note="1.6 kb mRNA"
polyA_site 6925..6928
/gene="ald"
/note="1.6 kb mRNA"
exon
7506..8865
/gene="ald"
/note="2.6 kb mRNA"
/number=3
/evidence=experimental
exon
7506..8275
/gene="ald"
/note="2.0 kb mRNA"
/number=3
/evidence=experimental
polyA_signal 8237..8242
/gene="ald"
/note="2.0 kb mRNA"
polyA_signal 8255..8260
/gene="ald"
/note="2.0 kb mRNA"
polyA_signal 8725..8730
/gene="ald"
/note="2.6 kb mRNA"
polyA_signal 8780..8785
/gene="ald"
/note="2.6 kb mRNA"
polyA_signal 8794..8799
/gene="ald"
/note="2.6 kb mRNA"
polyA_signal 8844..8849
/gene="ald"
/note="2.6 kb mRNA"
BASE COUNT 2733 a 1947 c
1857 g 2687 t
ORIGIN
1 tcaactttta atgagtatac cagatcgaac catatcgttt cttaccaaaa ctgggaaaac
61 aatttatcat tcttctgaac cacaaatgca ctcctcttat tcgtatctcc tctttaattt
121 aatatgagtt aaaacacagc tggtgattga atttcagact cttatgcact ccacgttcgg
181 ttcacataac tagaataatc agaaattatt tcgctttggc tgcaccacag tacgtagcgt
241 atacgccgcg gtgtacgcgg gtgtgcgaag gagccgcgca gcgcaagtgg aacgcaacac
301 gtgttgggcg acaggtgccg agcgtttggc acacaactcg accgaccgac gcacgaattc
361 tgaaaactga accagaatcc ccaaacgaac atgggaagcg agtgaggcag gcacgagaac
421 cgaaacagga cgaacgacga agccacgagg cacaaagagc gcgaggacga tgaagagcac
481 tgtggctaat gttccttcat taggtaagca tattatttca tatatatcta gcaactgtat
541 cactttaact aaagaaacct aagcatagat agtctatgaa atatctaaaa tcctttaaat
601 gttataaccc gttccacagt cattcttttc attcagaaaa ctgtgccaaa gtcatattag
661 gacattttaa agagcgaaac gatgttctct ttcgctcgaa cagattgtat ggcagaatag
721 ggggatattt tctcaaagtt tgcgcgctca atcagctgtt tccagtgccc gggggttgag
781 tcctttaaca ttgcaggagg tcaggcatgg cgtgaaagtc gcttatccta gctgctgctt
841 ctggcacccc tcgtttttgt tgtgctgccg ctggagcgcc tgatgctgat ggtccagaaa
901 aattcattga ccgccccaag taaagcaggt ccctcgtaat taaggttaaa tagacgccaa
961 ttaattattc taccctctct tcaatgcata agtagaatag gaaatgttgt tgacaggtta
1021 aaagatacaa atacttgcaa agataacact tcatttttaa tgctttttta tagttttggg
1081 caagaaagct tggcaagaca tatagttcac ttgatgagga tgatttggga tgactatatc
1141 cagcaagacg cgacattaag tttgtggcac tttattgttt ttaacatttc aatactattt
1201 caatatatat cgtatagtgt ttgagatctt aagataaatt atttattatg ttaatacgct
1261 ttgagcacat ttcatttaaa tttttaccgc ccaaacgaaa gctaaagctt ctcaaagctg
1321 tctaataagt tttacagctt atctgttagg ttccatcccc cactaaaagc tcccagaatg
1381 agctttattt tggatacctt ccgttcgggg atctgggaat ttaaagcgct gggctcagtc
1441 ggtggcccaa caccgtcggc gacgacctcc aaacaacctt ttggggtctc gctcggcatg
1501 tcgggcttat ttcgctttgg aatttcacgt tgacgccgca gtcagcggta gcagaggcat
1561 cggcagcggc agtagcagcg acacgggccg aaaataaaag cgttaaccgc tctcctccag
1621 tgcagcagca gcagcggacc cagccagtag ccagcagcta atcaccacat cccagattca
1681 gtttccagtt cggtgagtgg gcgtcataac gcagtgaaca gaacccctga gccccttaaa
1741 aaaaattcag atatttttcc ccccattggc ggccgtgctg ttgaaaagtc gcgtaatcgt
1801 tcccatttct ctgggtgttt cgtaatccaa atctacagag cagcagcgca tctgtaggcg
1861 tgttagtgtt agtgagtgcg tattcgaata tagtttaatt attctctaag tcttaccatt
1921 tccgcgagct cgccactttt atgacaagtt ctagaacctc gaggccctcg agtgttgttg
1981 acctatgcac cgcggttgaa ggagtggggg ggctacgcga actgcagcga taagaagcct
2041 tatcgggtga cttttggctc actttcgtga gtactagttt tctctaccgg ctcaaccggc
2101 tctcttactt ttcatccgct ctttcattcg agttcatttg cttgttggca ggctttcaac
2161 tgcaatttgt agtcgccgct gcggtttttc tttataaata gttggcgcaa atgttaaatg
2221 tatgtactat acatcgcgat atacatatgt atactttccc atcgctctct cgcttagact
2281 tcaattagcc aacgtcatcg taaattcatc agattgttga cgtcttctca tgatagacgc
2341 aatatgcttt tgtctattgc attttatacc taccatttaa ttgagatttc agtgaatgaa
2401 ggagagcaaa cattttaccg atagtttgta gagagtacat gagaaaagag gggcagaagt
2461 ttaagggttg ttttgaaacc tacagaaaac aagttttggt actccaaagg acttcctttc
2521 aagatcaacc tgattaggat ttttaagaag agttttgaac atttaaaaca accaatttat
2581 gttcctttga tcgtttatat caagctttgg gaaattttat aatgaatata atggaattat
2641 aggtatacat tctcattgta aagtataagc taatcgatga aataagacta gataaggtac
2701 attggcttat ctagtaaaca gttaccacaa caaagttttt ggggtcacat aataagggca
2761 tagatgaagt atgggaaggg ctcgaagttg cctattttca gacagtatac aattattacc
2821 acccatgctt tcggctaatc agttgacggc agttcgtagt agtagtagta gtattattct
2881 caactgttgg atgttctttt gacgcactgt ctgcgttcaa ggaggccgga cctaaaatct
2941 cgttatgatc atgcaaaacc tttggaacta aaacgcaact gggggcgggt tctttcgtcg
3001 ttttcgatcc aaaattttca ctgatcgcaa gctcacgaat acatatagac ggacatgtgg
3061 cggtttattt ttataaacgg tctcttgata ggaacgtaaa tttattttga ttgttttttg
3121 ttgtcatgcg ttggctgctc ttcgttcctc gttctttttt gtcatcgtat atatttcgcc
3181 tagtgttcga aacatttcag tcttcgattg attgccggca agtgtaggtg ggtgttgtct
3241 gctgagtgca tccgcaacat ctatagataa ttctatagat aagggtgtac atacatatct
3301 agtgtcgttc tgtgaagtgt gtttagataa ctgtttagaa attaatagat gaaaaaatga
3361 tttgtattct agagatatat taatatattc ttaaactgtg cagtattttt aatatatgca
3421 tatatcaagc ttttataata tctatctgtt cttaggaacc acaagctatt cacgcgtgta
3481 caaagtgtta aatatatggc gtgggaaatg tggggtcgat gtggtaaaca atatttcgcg
3541 ataagttgcg gaggtctaat ctcttaacct gacatttatg ccattcccag tccttggctc
3601 actgtcaatg tcaaacaagt ataaaatggg tttttagtca tcgcagatga gttcgtatct
3661 gtatctacat attgctggac actagctatc tctatcgcag ttcacacgcg ccacacgact
3721 ttcggttttc aacaatttct attcgcattt cgtaagcaac aaatttcgct ggcaacattt
3781 gttgctaagg cttgttgctt gaccttgttc aaggtcaagc gaaaattcat gcgccctgcg
3841 ttggcgtcgc tgcaggaaca gcgcagcagc agcggcaata aaagtgaaag ttaccacgtc
3901 acgcattatg cgggtgtgaa gaagacagct gcattcggtt agagtgagac acaagattcc
3961 atgactttga ttacatcaga gggaattatt gccgaggttt ccgtgacttt gctcagttac
4021 aaaaacatca tcgtcgaggt tggttcggat ttttctttgt gatctaagct taaaacaatt
4081 gttcggtttt tgcaaatagt ttttagcaaa ttacagacac tataaaataa gacactatat
4141 aatatttcat tccaaaaagt gcagtgatac ttttccaatt tgtatagtac aatccaagag
4201 gatgtttcta aaatgtgaac ctttcattga tgttcgtgag aacctctaaa aaagctctat
4261 taaaaggcaa cattttatat gtgaacgttg agagtgtagg acaaacctag aaaaaaaaaa
4321 atgtgagaga gagagagcga agagctgcag atatagagaa aagcacgttt tccgtgcatg
4381 cgcctttaat ctcattcaat cacccgctct ttgccatcga atcagctgtg aaatacacta
4441 ccatgcaaag catttattat cttcaatgga aaaatatttt taaattggaa aaaacaccag
4501 tgacattgac ctgacactga aaacaaaatt atataatacc gcatcattaa acaacagcat
4561 atgactcaat ggctctaatc ggttaactca gagttccact ttaaataact tgacctttac
4621 aaatatcttt ttattttatg gaaataataa ttaggtcagt tcagtaaaat aatccaacac
4681 ttgattgata gctatctctg tagccgttgt tatctttttc agtaggaaca tatgtaactt
4741 tgagttactt gattttggtt gtcagactgt gcggatcgta tacgaaattt tagtccaaat
4801 ttttagtttt attttttacc tcgaaatatt tcaaaattct ctgcttacgc aatggtttcc
4861 aatcatccgc gccgctgcgg aattcgatag ctagcttatt aaaacacaat gtccgctact
4921 caaaaacatc caacaaaata acaccacacc aaatcaaacg taaatggatc ttcgtgctgc
4981 agaactacac tcgaagctca aaaatgacga cctacttcaa ctaccccagc aaggagctgc
5041 aggatgagct gcgcgaaatc gcccagaaaa tcgttgcccc cggcaaggga atcctcgccg
5101 ccgatgagtc cggcccaacc atgggcaagc gtctgcagga catcggcgtg gagaacaccg
5161 aggacaaccg ccgtgcctac cgtcagctgt tgttcagcac tgaccccaag ctggccgaga
5221 acatctctgg agtgatcctg ttccacgaga ccctctacca gaaggccgat gatggcaccc
5281 ccttcgccga gatcctgaag aagaagggaa tcattctggg catcaaggtc gacgagggtg
5341 ttgtcccact gttcggctct gaggatgagg tcaccaccca gggtctggat gacctggccg
5401 cccgttgcgc ccagtacaag aaggacggtt gcgacttcgc caagtggcgt tgcgtcctga
5461 agatcggcaa gaacacccca tcctaccagt cgattctgga gaacgccaat gtcctggccc
5521 gctacgcctc catctgccag tcgcagcgca tcgtcccaat tgtggagccc gaggttctgc
5581 ccgatggcga tcacgatctg gacggcgccc agaaggtcac cgagaccgtc ctggcggccg
5641 tctacaaggc cctgagcgac caccacgtct acctggaggg tactctgctg aagcccaaca
5701 tggtcaccgc cggtcagtcg gccaagaaga acaccccaga ggagatcgct ctgaccaccg
5761 tgcaggctct gcgccgcacc gttcccgccg ccgttactgg cgtgaccttc ctgtctggag
5821 gtcagtccga ggaggaggcc accgtcaacc tgagtgccat caacaacgtt cccttgatcc
5881 gcccatgggc cctcaccttc tcgtacggtc gtgccctgca ggcctccgtc ctgcgtgcct
5941 gggctggcaa gaaggagaac attgctgccg gccagaacga gctgcttaag cgcgccaagg
6001 ttaggtcctt ggatagttgg aatctatgcg cggattgatt gcagttagca atttaagtgg
6061 tgaatgtatt aagagatgtc aggatatgct tcggaagtat tccttgggct aagaaatatt
6121 atacatgtat aatataacat acagcatatt cccagccatt gattccattt ccgatcccaa
6181 atgaccacaa tgttcttcta atcctctaat cttatccctt ttatttccct accattattt
6241 gtaacctttt tttgctttta gtacgtgaga cattaatttg gatgatccat aaccacacat
6301 taatcatgcc taggctaacg gagaggcggc ttgtggtaac tatacagctg gatcggttaa
6361 ggggttcgct ggaaaagata ctttgcatgt ggatgaccac aggtattgat ctaaggattg
6421 aagaattgct gccttaagat ggcgatgaac aagctttcat ggggtaactt aagaatcata
6481 gccgcctttc accaaatccg acttatcgca aacgccttta acatactgtc ctcgtgccac
6541 tcatgcttcg cttgctctaa acctaatcca caaaagccca aaatctgatt aatcccaacc
6601 aaaatcccgc tctcctcggg cttccacagg ccaattccca ggcctgtcag ggtatttacg
6661 tgcccggctc aattccgtcg tttgccggca atgccaacct ctttgtcgcc cagcacaaat
6721 actaagtcct aaatgccacg cactaatccc gagtcacaat cgagcgaagc tatatcccaa
6781 gccgaatcca gataatccgc aagagttact gatcgaggcc cagctggagt gagacgggca
6841 acatttatat tcctccaaca atggaactgc aaatatattc cttttataca tttttacatt
6901 ttaccgaata aactgactag accttaaaga aaatattttg ggcgaacaga aataaattat
6961 atatttctaa taataccttg cttatgattt tgtttacttt agttagcttt aaacaaaaat
7021 tcgatgttat tttacctttt aatttaattt aaatacattt taattaaaaa tgtcttgtct
7081 aactttattc atgaaggccc ttggcaaact tcctactgtt aattagaata ataattcgga
7141 atatatgtac ctgacagtta agttgaaaac aacgtgaaat tcgttcggag agctgttaca
7201 tatatttaat cgaatcataa ttttcctcca aagaacttgc aagattaaat tttccaaaac
7261 tttccgcagt acgcagcatg tattcgtgcg ctttgatgcg tggaaagttt ttagaaaatt
7321 gccgcaggtg gaaaaagtgg cggaagttct ttgagcgcag caaaaatcag tgttaaaact
7381 ttttcatact ggccgaaaaa cttttttgct gcggttcgcc aagttacaac taagtaataa
7441 ttaggagtag gcatttctca caacgaaatt aatcaatttt ccccgctttc tttaccctcc
7501 tccaggccaa cggtgatgct gctcagggca agtacgttgc cggcagcgct ggtgccggat
7561 ctggatccct gttcgtggcc aaccacgcct actaaggcgt ttggttggac cttggctaca
7621 cgttactaca aaaaaaaaaa aatacgaagc agattgctgg aaaagtttgg cagatttgtt
7681 tatttttacc ttagtttcga gagctaagca aagcaagcat tacaaaaaat aaccaaaaaa
7741 aaaagatgaa cgattacact ttcaaaacat gttatatata tgtagtacgt gacatttttg
7801 caagtttatt atattataac attattttag agaacaacta aatgatgagt gcagaagaaa
7861 ggattgaata tgttcgatgg agaaacaatt caacaattca aacaatttta tctttagttt
7921 ttaagcacat gaacctatta ttgttgaagt tattacattt ctattctatt attatgatta
7981 tgcaagattt caatgaaaac aaaaggatat gtcgtaaatc gctcggctaa agcacatttt
8041 atggtgaacg cgacagcaat cactatcagc aacacctaaa tgtatgaggc ttgtaacatt
8101 gacgccgcgc gccgtttttt gaattttttt aatgttttat aaaaagctcc actatgaaga
8161 tcggatgttt tccattatta ttattattaa actttatatt tatgattgat aagtataagt
8221 ggaaacaaat gacgagaata aacaaacaca ttaaaataaa cgcgaaaagc caccaaaaca
8281 gctttattaa ccttatatac atatatttac attgttaaaa gagaacttgt tgttgcaaaa
8341 tactaaaaga aaatatatga atttatatat tcaacatttg gacttcagtg tccaatggct
8401 tctaaactgt gctggggcac aatgcttgtt actagaattt ttttgaaacc aactctgatt
8461 ctctatccaa aacgcgatgg gtagtaacaa cggcagcaac gacattatca cagtacatat
8521 ttaaatacaa aaatattgta ccaatgacta acccacatat tatcacatca tgttttaatt
8581 tacacgttat tcaagttgca aattctgcat atcgttgtta ttcacacaat tatttgaagt
8641 gcaatgtcaa aatataacag acacgagtga aggatataca ataataccta ccggaagggt
8701 taagcttaaa atagtttatt gtgaaataaa aaccaccaca aacggcaaca acaaccagct
8761 gtacatccaa tatacaagaa ataaaaaaca acaaataaac atcatcggca tacaagtaat
8821 ggtgttaaag tgtaacatct ttcaataaaa aaccttaaga gcaaagaaac ccgaatttgg
8881 tttaatttta atgggttgtt ttaaaaagcg ttgcatacct ccaggcctga gttacagttt
8941 aaaaggtact gtgggcttaa aaccaagtca tctttatcat tgaggttaca tgtagcattc
9001 gaagttctta tatcactgaa gtcaaccagt aataaccttg ggcggttgct attaacatag
9061 ccaaaggtaa agccgcagac cttgaggcaa cgcagaatct gtacccaatt tcgagagttt
9121 tgtgttactt aaatttacaa tttcgcttat ttgtaatgca aatggaagca aaagcacagc
9181 gggcaaccgg gtttcgtaat cagcaccgtg atccttttaa ttgg
//