Schistosoma mansoni
LOCUS SCMPFK
3087 bp mRNA INV 09-SEP-1994
DEFINITION Schistosoma
mansoni phosphofructokinase (PFK) mRNA, complete cds.
ACCESSION L31531
VERSION L31531.1
GI:533267
KEYWORDS phosphofructokinase.
SOURCE Schistosoma
mansoni (library: lambda ZAP) adult cDNA to mRNA.
ORGANISM Schistosoma
mansoni
Eukaryota; Metazoa;
Platyhelminthes; Trematoda; Digenea;
Strigeidida; Schistosomatoidea;
Schistosomatidae; Schistosoma.
REFERENCE 1 (bases
1 to 3087)
AUTHORS Ding,J.,
Su,J.-G.J. and Mansour,T.E.
TITLE Cloning
and characterization of a cDNA encoding phosphofructokinase
from Schiftosoma
mansoni
JOURNAL Mol.
Biochem. Parasitol. 66, 105-110 (1994)
MEDLINE 95075381
FEATURES Location/Qualifiers
source 1..3087
/organism="Schistosoma
mansoni"
/db_xref="taxon:6183"
/dev_stage="adult"
/tissue_lib="lambda ZAP"
gene 147..2492
/gene="PFK"
CDS 147..2492
/gene="PFK"
/EC_number="2.7.1.11"
/codon_start=1
/product="phosphofructokinase"
/protein_id="AAA29911.1"
/db_xref="GI:533268"
/translation="MATWMEGKYVARGQFTGECIAVLTSGGDAQGMNAAVRAVVRMGI
YCGCRVFFIREGYQGLVDGGQNIQEASWADVSGILQLGGTKIGSARCMDFRERYGRLK
AAENLVKNQITNLVVIGGDGSLTGANLFRAEWSSLLEELVTSNKISAESAKQFHRLNI
VGLVGSIDNDFCGTDMTIGADSALHRIIEATDAISTTAHSHQRCFILEVMGRHCGYLA
LVASMACEADWVFIPEMPPTDDWREKLCHKLRMNREHGQRVNIIMVAEGAIDRACKPI
TCEIVKNLIVSELQLDTRITVLGHVQRGGSPSAFDRILGSRMGAEAVLALMDADRDPN
LPSCVISLDGNQAVRVPLVKCVDRTRQVAEAMKACDFDHAVELRGTSFMNNLATYIKL
SKIEQPRQSVMSSENNLRIGIVNVGAPACGINAVIRGFTRLGITKGYKVIGIHEGFSG
LVKGDASEIQWADVRGWVGMGGSMLGTRRDTPNGLGIDKVAAKFKELKLSGLLIIGGF
EAYECMIELVEGREKYPELCIPMAMVPATISNNVPGTDFSLGCDTALNEITSVLDKIK
QSALGTKRRVFVVETMGGYCGYLATMSALAGGADAAYIFEEPFTIDDLREDVVHLRAK
IDDNVKRGLVLRADMLINTITSEFIHQLYAQEGQGIFDCRCNVLGHMQQGDRPSPFDR
SLGTKFASKAIDWLDEQINANIVQILQSIHQTFMLYINWYCTSSDNLFKYSLELREHT
DFVHRLPKEEWWLSLRPLMRIMAKHDSLYESESIMAGTDRK"
BASE COUNT 975 a 447 c 639 g 1026 t
ORIGIN
1 ctgttgttta tggtatctgt agattaacac aactaaaggc ttcaagtcat
cgtttggatt
61 ttcagaaata aagctttgac gtttactatt tgataaacat tcagacacga
tatggaatgt
121 agaaggtttg agcgccgagc aacagaatgg ctacctggat ggaaggtaaa
tatgttgccc
181 gaggacaatt tacgggtgaa tgtatcgctg ttcttacgag cggtggagat
gcacaaggaa
241 tgaatgcagc tgtacgagct gtagtccgta tgggaatata ctgtggatgt
cgagtatttt
301 tcatcagaga agggtatcaa ggtcttgtgg atggtggtca aaacattcag
gaggcatcgt
361 gggcagacgt ctctggtatt ctccaattgg gaggtactaa gattgggtca
gctcgatgta
421 tggattttcg tgaacgttat ggacgtttaa aagctgctga aaatctagtc
aaaaatcaaa
481 taaccaattt agtggttatt ggcggtgatg gttctttaac tggtgctaat
ctatttcgag
541 ctgaatggtc aagtctattg gaagaacttg tcacatcaaa taaaattagt
gcagaaagtg
601 ccaaacaatt tcatcgttta aatattgtcg gtttagtcgg tagtattgac
aatgattttt
661 gtggtacaga tatgacaatt ggtgcagact cagcattgca tagaattatt
gaagcgactg
721 atgcaatctc tactacagct cactcacatc aaagatgttt cattttagag
gtgatgggta
781 gacattgtgg ctatctagca cttgtcgcat ctatggcgtg tgaagctgat
tgggtattta
841 taccagaaat gccaccaact gatgattggc gtgaaaaatt atgtcataaa
cttcgaatga
901 atcgtgaaca tggacaacgt gtcaatataa ttatggttgc tgaaggtgct
atcgatagag
961 catgtaaacc gatcacttgt gaaatagtga aaaatttaat agtatcagaa
cttcaacttg
1021 atacacgtat aactgttttg ggtcatgtcc aacgtggtgg ttcaccatct
gcatttgatc
1081 gtattttggg cagtcgtatg ggagctgaag ctgttctagc cctaatggat
gctgatcgtg
1141 atcctaatct accatcatgt gttattagtt tggatggtaa ccaagcagtt
cgtgtaccac
1201 ttgtgaaatg tgttgacaga acacgtcagg ttgctgaagc aatgaaagct
tgtgattttg
1261 atcatgccgt tgaactacgt ggaacgagtt tcatgaacaa tttagcaacg
tatataaaac
1321 tttcaaaaat tgaacaacca cgtcaaagtg tcatgtcatc tgaaaacaac
ttgagaatcg
1381 gtatcgtgaa tgtcggtgct ccagcttgcg gtattaatgc agtaataaga
ggatttactc
1441 gtttgggtat cactaaaggt tataaggtta tcggaataca cgaaggattt
tccgggcttg
1501 ttaaaggtga tgctagtgaa atacagtggg ctgatgtccg tggatgggtg
ggaatgggtg
1561 gttctatgtt aggaacacgt agagatacac cgaatggttt gggtatagat
aaagtagcgg
1621 cgaaatttaa agaattaaaa ttaagtggtc tacttattat tggtggcttt
gaagcgtacg
1681 aatgtatgat agaactggtt gaaggacgtg aaaaatatcc tgaattatgt
atacctatgg
1741 ctatggtacc agcgacaatt tctaataatg ttcctggtac tgatttctct
ttgggttgtg
1801 atacagcttt aaatgaaata acctctgttt tagataaaat taaacaaagt
gcattaggta
1861 caaaacgtcg tgtatttgtt gttgaaacaa tgggcggtta ttgtggttat
ttagctacaa
1921 tgagtgcatt ggctggtgga gcagatgctg cttatatatt tgaagaacca
tttacaattg
1981 atgatttacg tgaagatgta gtacatttac gtgcaaaaat tgatgataat
gttaaacgtg
2041 gtttagtact acgagctgat atgctaataa atactataac aagtgaattt
attcatcaat
2101 tatatgctca agaaggtcaa ggtatatttg attgccgttg taatgtacta
ggtcatatgc
2161 aacaaggtga tagaccaagt ccatttgata gaagtttagg tacaaaattt
gcatcgaaag
2221 caattgactg gttagacgaa caaatcaacg ctaatatcgt tcagattcta
cagtctatac
2281 accagacatt tatgttgtac attaattggt attgtacgtc gtcagacaac
ttattcaaat
2341 attcactgga attaagagaa catacagatt tcgtacatcg tttaccaaaa
gaagaatggt
2401 ggcttagttt acgtccatta atgcgtatta tggctaaaca tgatagtctt
tatgaatcag
2461 aaagtattat ggctggtact gatagaaaat aattactttt tcttttatca
tacatatttc
2521 aacttattca atattagttt tgcttaattt tcattcataa aatcagatct
tattattatt
2581 gttaatatcg ataatttttt tgtctttttc ttgttgtttt cttcactgat
ttattcgttt
2641 acttagttac tacttatggt aattcaacca accttgtcca tcttattctg
ctttattgaa
2701 gattaatcaa taatatgtat tgtaacccta caagtttagt taaattcaat
attataaatc
2761 agtcatttta acctttcaaa accaacaccg atcgccttta ctatagttgt
agaaaatgaa
2821 atttaattta gccctttttt ttcaagaaaa aaaaggagaa aaacaaagta
aagaaaaacg
2881 aaagttattg ctaacatgtt cctttcattc tgtttttttt tccttttttt
gtcttttagt
2941 gagggtatgt agtattgtcc tagtgcaata tttaaagttt tgtcatgttt
tccgtttttt
3001 ttttgtacaa cagagaaatt atataataaa cactttgatt ttcatgtaaa
aaaaaaaaaa
3061 aaaaaaaaaa aaaaaaaaaa aaaaaaa