LOCUS BOVPGF 1220 bp mRNA MAM 27-APR-1993
DEFINITION Bovine lung prostaglandin synthase (PGF) mRNA, complete cds.
ACCESSION J03570
VERSION J03570.1 GI:163511
KEYWORDS prostaglandin synthase; rho-crystallin.
SOURCE Bovine lung, cDNA to mRNA, clones pPF[13,41,131].
ORGANISM Bos taurus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia;
Eutheria; Cetartiodactyla; Ruminantia; Pecora; Bovoidea; Bovidae;
Bovinae; Bos.
REFERENCE 1 (bases 1 to 1220)
AUTHORS Watanabe,K., Fujii,Y., Nakayama,K., Ohkubo,H., Kuramitsu,S.,
Kagamiyama,H., Nakanishi,S. and Hayaishi,O.
TITLE Structural similarity of bovine lung prostaglandin F synthase to
lens epsilon-crystallin of the European common frog
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 85, 11-15 (1988)
MEDLINE 88124772
COMMENT Draft entry and printed copy of sequence for [1] kindly provided by
O.Hayaishi, 04-DEC-1987.
Bovine prostaglandin synthase has extensive amino-acid sequence
identity to a frog (genus Rana) eye lens protein (crystallin). The
protein was initially named epsilon-crystallin, but is now known as
rho-crystallin. PGF does NOT share sequence identity with the
epsilon-crystallin found in Anseriforms (ducks).
FEATURES Location/Qualifiers
source 1..1220
/organism="Bos taurus"
/db_xref="taxon:9913"
mRNA <1..1220
/product="PGF mRNA"
CDS 7..978
/note="lung prostaglandin F"
/codon_start=1
/protein_id="AAA30694.1"
/db_xref="GI:163512"
/translation="MDPKSQRVKLNDGHFIPVLGFGTYAPEEVPKSEALEATKFAIEV
GFRHVDSAHLYQNEEQVGQAIRSKIADGTVKREDIFYTSKLWCNSLQPELVRPALEKS
LQNLQLDYVDLYIIHSPVSLKPGNKFVPKDESGKLIFDSVDLCHTWEALEKCKDAGLT
KSIGVSNFNHKQLEKILNKPGLKYKPVCNQVECHPYLNQSKLLEFCKSHDIVLVAYAA
LGAQLLSEWVNSNNPVLLEDPVLCAIAKKHKQTPALVALRYQVQRGVVVLAKSFNKKR
IKENMQVFDFELTPEDMKAIDGLNRNIRYYDFQKGIGHPEYPFSEEY"
BASE COUNT 362 a 252 c 286 g 320 t
ORIGIN 9 bp upstream of BamHI site.
1 caaacaatgg atcccaaaag tcagagggtg aagcttaatg atggccactt cattcctgtc
61 ctgggatttg gaacctatgc acctgaggag gttcctaaga gtgaagccct ggaggccacc
121 aaatttgcta tagaggttgg gttccgccat gtggacagtg ctcatttgta tcaaaatgag
181 gagcaggttg gccaggccat tcgaagcaag attgcagatg gcactgtgaa gagagaagac
241 atattctaca cttcaaagct ttggtgcaat tcccttcaac cagagttggt ccgaccagcc
301 ttggaaaagt cattgcaaaa tcttcaactg gactatgtcg atctctatat tattcattct
361 ccagtgtctc tgaagccagg gaataaattt gttccaaaag atgaaagtgg aaaactgata
421 tttgactcgg tggatctctg tcacacgtgg gaggccctgg agaagtgtaa ggacgcaggg
481 ctgaccaagt ccattggggt gtccaacttc aaccacaagc agctggagaa gatcctgaac
541 aagccggggc tcaagtacaa gcccgtctgc aaccaggtgg aatgtcaccc ttacctcaac
601 cagagcaaac tgttagagtt ctgcaagtca catgatattg tcctagttgc ttatgctgct
661 ctgggagccc aactattgtc agaatgggtg aactcaaaca accccgttct cttggaggac
721 ccggttcttt gtgccattgc caaaaagcac aagcaaaccc cagctctggt tgcccttcgc
781 taccaggtac aacgtggagt tgtggttctg gccaagagtt tcaacaagaa gaggatcaaa
841 gagaatatgc aggtgtttga ctttgaactg actccggaag atatgaaagc aatcgatggc
901 ctcaatcgta atataagata ctatgatttt caaaagggta ttggtcaccc tgagtaccca
961 ttttctgaag aatattaact gggtgagctg tccaccatgg cttctacctg aacgtctgct
1021 tctagggcta cgaagagcgt gtctatactt ggtggaggtg tttaaaagaa gtgcctgaac
1081 ttttgaaaga ttgtttttct ttaaaaactc tttatgaaat aaccaagatt tcaaatatgg
1141 gtactagttt ttcctaacaa aataatttga aaaataaaag ggaaaagata gaaaataaag
1201 ataacttggt taacttactt