LOCUS ANI9973
2463 bp DNA
PLN 08-JAN-1999
DEFINITION Aspergillus niger hxk gene.
ACCESSION AJ009973
VERSION AJ009973.1 GI:4140254
KEYWORDS hexokinase; hxk gene.
SOURCE Aspergillus niger.
ORGANISM Aspergillus niger
Eukaryota; Fungi; Ascomycota; Euascomycetes; Plectomycetes;
Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
REFERENCE 1 (bases 1 to 2463)
AUTHORS Panneman,H.
TITLE Direct Submission
JOURNAL Submitted (31-JUL-1998) Panneman H., Molecular Genetics
of
Industrial Microorganisms, Wageningen Agricultural University,
Dreijenlaan 2, 6703 HA, THE NETHERLANDS
REFERENCE 2 (bases 1 to 2463)
AUTHORS Panneman,H., Ruijter,G.J., van den Broeck,H.C. and
Visser,J.
TITLE Cloning and biochemical characterisation
of Aspergillus niger
hexokinase--the enzyme is strongly inhibited by physiological
concentrations of trehalose 6-phosphate
JOURNAL Eur. J. Biochem. 258 (1), 223-232 (1998)
MEDLINE 99067038
FEATURES
Location/Qualifiers
source 1..2463
/organism="Aspergillus niger"
/strain="N400"
/db_xref="taxon:5061"
/chromosome="6"
mRNA
join(317..356,447..569,652..677,767..1174,1239..2114)
/gene="hxk"
/product="hexokinase"
/evidence=experimental
gene
317..2114
/gene="hxk"
exon
317..356
/gene="hxk"
/number=1
CDS
join(317..356,447..569,652..677,767..1174,1239..2114)
/gene="hxk"
/EC_number="2.7.1.1"
/function="phosphorylation of glucose and fructose"
/codon_start=1
/evidence=experimental
/product="hexokinase"
/protein_id="CAA08922.1"
/db_xref="GI:4140255"
/translation="MVGIGPKRPPSRKGSMADVPQNLLQQIKDFEDQFTVDRSKLKQI
VNHFVKELEKGLSVEGGNIPMNVTWVLGFPDGDEQGTFLALDMGGTNLRVCEITLTQE
KGAFDITQSKYRMPEELKTGTAEELWEYIADCLQQFIESHHENEKISKLPLGFTFSYP
ATQDYIDHGVLQRWTKGFDIDGVEGHDVVPPLEAILQKRGLPIKVAALINDTTGTLIA
SSYTDSDMKIGCIFGTGVNAAYMENAGSIPKLAHMNLPADMPVAINCEYGAFDNEHIV
LPLTKYDHIIDRDSPRPGQQAFEKMTAGLYLGEIFRLALMDLVENRPGLIFNGQDTTK
LRKPYILDASFLAAIEEDPYENLEETEELMERELNIKATPAELEMIRRLAELIGTRAA
RLSACGVAAICTKKKIDSCHVGADGSVFTKYPHFKARGAKALREILDWAPEEQDKVTI
MAAEDGSGVGAALIAALTLKRVKAGNLAGIRNMADMKTLL"
intron 357..446
/gene="hxk"
/number=1
exon
447..569
/gene="hxk"
/number=2
intron 570..651
/gene="hxk"
/number=2
exon
652..677
/gene="hxk"
/number=3
intron 678..766
/gene="hxk"
/number=3
exon
767..1174
/gene="hxk"
/number=4
intron 1175..1238
/gene="hxk"
/number=4
exon
1239..2114
/gene="hxk"
/number=5
BASE COUNT 532 a 767 c
601 g 563 t
ORIGIN
1 ctcgagatac tttgatgcaa gaggacaaga catgaagcgg cgagacaccc aaagcaacaa
61 ccggtgaagg cgatccctcc cctccactgc ggggcccaac tttagttcaa ctattattat
121 tattattatt gtttgcttcc acgcttatga ttctgctgct gccccacgtc actccccctc
181 gatcttcttt catcccggtt tctaattcta actctctctg ctccatcttc tccttgactt
241 ctctacattc ttctttttga tcaaactatc cttctctact acctctttct ccatctaata
301 tctattcacc gtcatcatgg ttggaatcgg tcctaagcgt cccccctccc gcaagggtaa
361 actcatagct ctcctcctcc cacttccccc ctccatttct cgggatgccg atcgctgacc
421 cgatggcttc cctcgtgctt ctccaggttc catggccgat gttccccaga acctcttgca
481 gcagatcaag gacttcgagg accaattcac cgtcgatcgc tccaagctca agcagattgt
541 caaccacttt gtcaaggaat tggaaaaggg tgcgtcttcc acactttacc cgcttccaat
601 gcggccccgg aatgcatcac tccgactgac tctctttctt ccatgcaata ggtctctctg
661 tcgagggtgg aaacatcgta agtgctttgg ctctcgcaat gcggcattcg actcaattcg
721 tttttgtttc ctccgttctc gactaacgct tctcttcccc tctcagccta tgaacgtcac
781 ctgggttctg ggattccccg atggcgacga acagggtact ttcctcgccc tcgacatggg
841 tggcaccaac ctgcgtgttt gtgagatcac cctgacccag gagaagggtg ccttcgacat
901 cacccagtcc aagtaccgca tgcccgagga attgaagacc ggtaccgccg aggagctgtg
961 ggaatacatc gccgactgcc tgcagcaatt catcgagtcc caccacgaga acgagaagat
1021 ctccaagctg cccctgggtt tcaccttctc ctaccccgcc acccaggatt acatcgacca
1081 cggtgtcctg cagcgctgga ccaagggttt cgacattgat ggtgtcgagg gccacgacgt
1141 cgtcccgccg ttggaggcca tcctgcagaa gcgcgtacgt cgaattcccc tccaccccca
1201 tgatgtgtaa cccgagctaa tctggtatgt ctctctaggg cctgcccatc aaggtggctg
1261 cactgatcaa cgacaccacc ggaaccctca tcgcctcttc ttacaccgac tccgacatga
1321 agatcggctg catcttcggt accggtgtca acgccgccta catggagaac gccggctcca
1381 tccccaagct ggctcacatg aacctgcccg ccgacatgcc cgtggctatc aactgcgagt
1441 acggtgcttt cgacaacgag cacatcgtgc tgcctctgac caagtacgac cacatcatcg
1501 accgcgactc gccccgtccc ggtcagcagg ccttcgagaa gatgaccgcc ggtctgtacc
1561 tgggtgagat cttccgtctg gccctgatgg acctggtgga gaaccgcccc ggcctcatct
1621 tcaacggcca ggacaccacc aagctgcgca agccctacat cctggatgcc tccttcctgg
1681 cagccatcga ggaggacccc tacgagaacc tggaggagac cgaggagctc atggagcgcg
1741 agctcaacat caaggccacc ccggcggagc tggagatgat ccgccgcctg gccgagctga
1801 tcggtacgcg tgccgctcgc ctgtcggcct gcggtgttgc cgccatttgc acgaagaaga
1861 agatcgactc gtgccacgtt ggtgccgacg gctccgtctt caccaagtac cctcacttca
1921 aggcgcgcgg agccaaggct ctgcgcgaga tcctggactg ggctccggag gagcaggaca
1981 aggtgaccat catggcggcc gaggatggat ctggtgtggg agctgcgctg attgcggcgc
2041 tgaccctgaa gcgggtcaag gccggcaacc tggccggtat ccgaaacatg gctgacatga
2101 agaccctgct ataatgacct ggcgcacata aatatgaatt ctttggtgtg atattttatt
2161 ctctggtgtt gagacggcgg tggacggaat gaaaataatt gattacagtt gttatgaaga
2221 attggcccgg cctggtctgg tctggagcaa tccttgtact gattaaaaag taagccagta
2281 agccagtgaa tgtgtaactc tcttgttcct atcgactaga cctgccacac taccatcctg
2341 tagacacaaa accccgcagc aaacaagccc tgagcaatgc aagcggaagg caaaaaagaa
2401 agaatgtgca cggtcactca gttggccagt gttgacttgg ttgcacagga agaatgggga
2461 tcc