LOCUS ECENO
2089 bp DNA
BCT 15-APR-1997
DEFINITION E.coli eno gene.
ACCESSION X82400
VERSION X82400.1
GI:563867
KEYWORDS eno gene; enolase.
SOURCE Escherichia
coli.
ORGANISM Escherichia coli
Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
Escherichia.
REFERENCE 1 (bases 1 to
2089)
AUTHORS Klein,M.,
Sprenger,G.A. and Freudl,R.
TITLE
Cloning, nucleotide sequence, and functional expression of the
Escherichia coli enolase (eno) gene in a temperature-sensitive eno
mutant strain
JOURNAL DNA Seq. 6,
315-355 (1996)
REFERENCE 2 (bases 1 to
2089)
AUTHORS Freudl,R.
TITLE
Direct Submission
JOURNAL Submitted
(27-OCT-1994) R. Freudl,
Inst.f.Biotech.1,Forschungszentrum Julich, Forschungszentrum
Juelich, GmbH, D-52425, Juelich, FRG
FEATURES
Location/Qualifiers
source
1..2089
/organism="Escherichia coli"
/strain="K12"
/sub_strain="JM109"
/db_xref="taxon:562"
RBS
13..17
gene
25..1338
/gene="eno"
CDS
25..1338
/gene="eno"
/EC_number="4.2.1.11"
/note="enolase"
/codon_start=1
/transl_table=11
/product="phosphopyruvate hydratase"
/protein_id="CAA57795.1"
/db_xref="GI:563868"
/db_xref="SWISS-PROT:P08324"
/translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEK
KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
NDEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
IANSYLIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
KTGSMSRSDRVAKYNQLIRIEEALGEKARTTVVKRSKARHKTDFI"
BASE COUNT 605
a 485 c 497 g 502
t
ORIGIN
1 aactagtgac ttgaggaaaa cctaatgtcc aaaatcgtaa aaatcatcgg tcgtgaaatc
61 atcgactccc gtggtaaccc gactgttgaa gccgaagtac atctggaggg tggtttcgtc
121 ggtatggcag
ctgctccgtc aggtgcttct actggttccc gtgaagctct ggaactgcgc
181 gatggcgaca
aatcccgttt cctgggtaaa ggcgtaacca aagctgttgc tgcggtaaac
241 ggcccgatcg
ctcaggcgct gattggcaaa gatgctaaag atcaggctgg cattgacaag
301 atcatgatcg
acctggacgg caccgaaaag aaatccaaat tcggcgcgaa cgcaatcctg
361 gctgtatctc
tggctaacgc caaagctgct gcagctgcta aaggtatgcc gctgtacgag
421 cacatcgctg
aactgaacgg tactccgggc aaatactcta tgccggttcc gatgatgaac
481 atcatcaacg
gtggtgagca cgctgacaac aacgttgata tccaggaatt catgattcag
541 ccggttggcg
cgaaaactgt gaaagaagcc atccgcatgg gttctgaagt tttccatcac
601 ctggcaaaag
ttctgaaagc gaaaggcatg aacactgctg ttggtgacga aggtggctat
661 gcgccgaacc
tgggttccaa cgatgaagct ctggctgtta tcgctgaagc tgttaaagct
721 gctggttatg
aactgggcaa agacatcact ttggcgatgg actgcgcagc ttctgaattc
781 tacaaagatg
gtaaatacgt tctggctggc gaaggcaaca aagcgttcac ctctgaagaa
841 ttcactcact
tcctggaaga actgaccaaa cagtacccga tcgtttctat cgaagacggt
901 ctggacgaat
ctgactggga cggtttcgca taccagacca aagttctggg cgacaaaatc
961 cagctggttg
gtgacgacct gttcgtaacc aacaccaaga tcctgaaaga aggtatcgaa
1021 aaaggtatcg
ctaactccta cctgatcaaa ttcaaccaga tcggttctct gaccgaaact
1081 ctggctgcaa
tcaagatggc gaaagatgct ggctacactg cagttatctc tcaccgttct
1141 ggcgaaactg
aagacgctac catcgctgac ctggctgttg gtactgctgc aggccagatc
1201 aaaactggtt
ctatgagccg ttctgaccgt gttgctaaat acaaccagct gattcgtatc
1261 gaagaagctc
tgggcgaaaa agcacgtaca acggtcgtaa agagatcaaa ggccaggcat
1321 aagactgact
ttatctgatt taaaaactag ctccgggctg gcatttttta tggttgagtt
1381 accacgggcc
ggatgcgccg ccacctccag aagaaccgcc cccgccgcta aatccgccgc
1441 tatctgagcc
tccagcggaa ccgctgttgt ctgaatgata acttctacca gatgctcttt
1501 tctgaagtac
acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa
1561 atacagcaat
ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg
1621 aaatacagac
tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga
1681 aagggaagaa
catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag
1741 acgctgattc
actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat
1801 tcaaagcgtt
tatagctagc tctaatccct tagctaattt ttgttgttta aatgcgggta
1861 tcatgttgct
acggatgata tctccagcca gagcatcggt taccttttct tccagcccat
1921 aacctacctg
gatgcggaca gtgcgatccg accaggcaac aacgatcagt atcccatcat
1981 tacgtttggc
atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg
2041 tctcgtcttt
ggttgtggga acaattaata cggcaatttg cacttggaa
//