LOCUS DROPFK
7101 bp DNA
INV 23-FEB-1995
DEFINITION Drosophila melanogaster phosphofructokinase (pfk)
gene, complete
cds.
ACCESSION L27653
NID g514918
KEYWORDS phosphofructokinase.
SOURCE Drosophila melanogaster (strain
Oregon) adult DNA.
ORGANISM Drosophila melanogaster
Eukaryotae; mitochondrial eukaryotes; Metazoa; Arthropoda;
Tracheata; Insecta; Pterygota; Diptera; Brachycera; Muscomorpha;
Ephydroidea; Drosophilidae; Drosophila.
REFERENCE 1 (bases 1 to 7101)
AUTHORS Currie,P.D. and Sullivan,D.T.
TITLE Structure and expression of the
gene encoding phosphofructokinase
(PFK) in Drosophila melanogaster
JOURNAL J. Biol. Chem. 269 (40), 24679-24687 (1994)
MEDLINE 95014229
COMMENT On Jul 23, 1994 this sequence version
replaced gi:471780.
FEATURES
Location/Qualifiers
source
1..7101
/organism="Drosophila melanogaster"
/strain="Oregon"
/db_xref="taxon:7227"
/dev_stage="adult"
TATA_signal 698..704
exon
719..1048
/number=1
5'UTR
719..901
gene
join(902..1048,2070..2414,2472..2635,3008..3110,
4291..4476,4663..5102,5160..5999,6079..6217)
/gene="pfk"
CDS
join(902..1048,2070..2414,2472..2635,3008..3110,
4291..4476,4663..5102,5160..5999,6079..6217)
/gene="pfk"
/codon_start=1
/product="phosphofructokinase"
/db_xref="PID:g514919"
/translation="MNSEINQRFLARGSQKDKGLAVFTSGGDSQGMNAAVRACVRMAI
YLGCKVYFIREGYQGMVDGGDCIRKSNWASVSSIIPFVGWHHPLLRPLPDFRERQGRL
KAANNLIQRGITNLVVIGGDGSLTGANLLPQEWSSLLDELVKNKTITTEQQEKFNVLH
MLGLVGSIDNDFCGTDMTIGTDTALHRIIEQSKAKVQSPVQPNSHQRTFIMEGQVSHC
GYLALVGGLACEAIHIHPNAPGRLGQTGSALSWTQRSAGQRLNIVIVAEGAMDREGHP
ITAEDVKKVIDERLKHDARITVLGHVQRGGNPSAFDRFLACRMGAEPLWPLMEATKDS
VPVVISLDGNQAVRVPLMECVERTQAGKAMAEKRWADAVNVRGRSFERNLETYKMLTR
LKPPKENFDADGKGIEGYRLAVMHIGAPACGMNAAVRSFVRNAIYRGDVVYGINDGVE
GLIARNVRELGWSDVSGWVGQGGAYLGSKRTLPEGKFKEIAARLKEFKIQGLLIIGGF
ESYHAAGQIADQRTTTHSFCIPIVVIPSTISNNVPGTEFSLGCDTGLNEITEICDRIR
QSAQGTKRRVFVIETMGGYCGYLPPLAGLAGGADAAYIYEEKFSIKELQQDVYHMPPR
WPRRLPRSNPANEKASENYSTDFIYRLYSEEGKGLFTCRMNILGHMQQGGSPTPFDRN
MGTKMAAKCVDCWPPRSRRTSTANGVVNCKSPDTATLLGIVSRQYRFSPLVDLIAETN
FDQRIPKKQWWLRLRPLLRILAKHDSAYEEEGMYITVEEECDTDAVA"
intron
1049..2069
/number=1
exon
2070..2414
/gene="pfk"
/number=2
intron
2415..2471
/number=2
exon
2472..2635
/gene="pfk"
/number=3
intron
2636..3007
/number=3
exon
3008..3110
/gene="pfk"
/number=4
intron
3111..4290
/number=4
exon
4291..4476
/gene="pfk"
/number=5
intron
4477..4662
/number=5
exon
4663..5102
/gene="pfk"
/number=6
intron
5103..5159
/number=6
exon
5160..5999
/gene="pfk"
/number=7
intron
6000..6078
/number=7
exon
6079..>6217
/gene="pfk"
/number=8
polyA_signal 6452..6457
polyA_signal 6545..6550
polyA_signal 6773..6778
BASE COUNT 1809 a 1651 c
1623 g 2018 t
ORIGIN
1 agcttataga ttcagcttgt
accatgccag gaataacatg aggattaacg cgcacgttca
61 tttctgggtc tgtcaccggc gcggctaaat
ttacaacttg ctgccagaat gtgccgccaa
121 gaaccgcggt ttgtgccgga cgcacaacgc
cttaacccaa ggcaaccaag ctgcagctgc
181 ctcctccaag caccaccgga gtgcccgcct
tagcagccag cagtttccgc cgctttgtga
241 acttaccacg cccagcaatg tgccggtttc
tttgacagga gaaagaatat cggtcatcgt
301 aggccagcca tatccagcaa tgcaggtttc
cagtcacggg tggttagatc aagaagtccc
361 gtggtgccag cgttagaggg atcaacagct
gaacgattgt gggtcgaacg taaacaaatt
421 ggttaaacgc tattttccgc tcgttgctag
tcgcgaaagt cgttcataac agacagaatc
481 ctcagatttt cgacctaaat caaaccaagt
tagggcaatc gcgttggagc gaactcttgg
541 gcggatttag ccatccgaag tttgcattgc
atcagtacgc acttacagaa agcccgtggc
601 tgtgtgctgt gtccgtgtgt gttgtgtatg
cgtctctggc ctgaaggtca ccttgaagct
661 caaagcaaag cgaactgtga ataccgcctg
cgagcggtat atattgctag taactgaaaa
721 gccacggcaa ggagacgtag tagtagtatt
tatagcccac ttgatataac ccagctccag
781 cggcgctgct cgcatagttg caaaactccc
taccgagtac aatagtggtt catagtgtgt
841 gctttgtgcg atatccgtta cgaccccagt
ttgatttaca tatagtgcaa aagattgaaa
901 aatgaattcc gagattaatc aacgattcct
ggcccgcggc tcgcagaagg acaaaggact
961 ggcggtcttc accagcggcg gggattccca
gggcatgaat gccgccgtgc gagcctgtgt
1021 ccgtatggcc atctatttgg gctgcaaggt gagttggtga
cctctgtcaa gtacttccgc
1081 ccacgcagat cgcgtccggt gaccccgatc ccaggcacag
gcaacaaacc catagtgaac
1141 ccagaaattt cgtgaaacac ctggtaattt gcttatcagc
agccgttaaa ccacgattat
1201 tgggccgcaa agccagttag ataatctcca atcggtgcga
aaagaaattc aagaatatat
1261 gtgtacatgc acattgacat tgggtttatt ttcgggtcct
cacatgcgat tttcgatttt
1321 cggctaattt gtttcgtttt tttgttgtcg ctcttggttt
ctattcggtt tatttaaatg
1381 agttctttaa tttgttaggg aacttcttgc ccggccgttg
gctccctcga aatatcgaaa
1441 atgtgtcaat taacgtagcc aatttaaatt gataaactat
tcatttgatt caatttctgg
1501 ggcataaagt aaagtattgc tgccttgcat gaccagcgct
tgaatatggt tcaataaatt
1561 gacaaattaa taaatatatc atggtatgct aaacaaatgg
ttcctaatca gttcaataat
1621 ctaacctttg tttttaatct actagtgcct tggcatcatt
attgtttatg ttattttttc
1681 acttttcaaa ttaagagcgt tataaaattt gaattccctt
ggttatctga gttgcttaaa
1741 aagtatcgat ttaacatcga ttacattgta attacctgta
acggtatttt gaatatgcaa
1801 atgttttgtt gtttaaaact taaacttatc agtaaaagtt
gagaaaagta tatgagataa
1861 gtcatatcat attaatttct attgactgtt ctttactgac
ttcctattaa atattacgac
1921 tttggcagag tgaaatgaaa ttaaataatt tatactcgaa
ttcggtgatt catttttaat
1981 tgtgctaatg atgtcatgtg ccatgaagat accaaactct
attctgtttt acaccatatc
2041 tacaattgat tcgtgtttac ttcggcaagg tctacttcat
tcgtgaaggc taccagggca
2101 tggtggatgg tggcgactgc atcaggaagt ccaactgggc
ctcagtctca tccattattc
2161 cattcgttgg ttggcaccat ccattgctcc gccctttgcc
agacttccgt gagcgtcagg
2221 gtcgcttgaa agccgctaac aacctgattc agcgaggaat
caccaatctg gtggtcattg
2281 gaggcgatgg ctccctcacg ggcgccaatc tgttgcctca
ggagtggtcc agcctgttgg
2341 acgagctggt caagaacaag accattacca ccgagcagca
ggaaaagttc aatgtcctgc
2401 acatgcttgg attggtatgc tacacagagt attccttgga
tatattttct tatggatgaa
2461 tctttttgta ggttggctcc atcgacaacg atttctgtgg
cactgacatg accatcggca
2521 cggacacggc actgcatcgc atcatcgagc aatcgaaggc
taaggtccaa tctccagtac
2581 agcctaactc ccatcagcgc accttcatca tggaaggtca
ggtgtctcat tgcgggtaag
2641 ttgatctctg agcggatatt gcgaggaaaa gttggatgcc
tctgtattag cctaagtgtc
2701 agtgattaaa ttgggatttt agctaatgcc tggaacgact
atctctagct atctttcggt
2761 ggtagctggc attatctccg aaggcaagac tacgtctttc
tgcccgagtc tcctccgcag
2821 ccgattggcc cgatcgactt gtccttaaat tggaacaggc
aagcccaaaa cggaaatctg
2881 ttgaatgtcg tcgactcatt tcgcacattt ctgtttctat
ggccgcagta ctatgtcgat
2941 cgatccccat atcctccaaa atcccatccc ccttttgact
aatattttgt aatatatatt
3001 tttgcagcta tttagctctt gtgggtggac tggcgtgcga
ggcgattcat attcatccga
3061 atgcgccagg tcgattaggc cagacaggct ctgctctcag
ctggacccag gtcaggaaga
3121 tgagctccat ttccattcca tcaagtcact ggagaagtcc
gaaaggcatc ggaaatatgt
3181 tctcgagaga cttggtatag gatttaggat attcttatta
atatagccag ttagctgtcg
3241 aagataatat tcctgtttga gatagagttg tatacttcag
tgcagttgtc gttgcttgat
3301 gcttaagtag tacacctgta cacaattttt tgaaagacac
attttaagag cgacacttgc
3361 catacaaagc ttggttctta tttctgctta ctgcacacga
atgtatcttt aaacacaaac
3421 cggagatacg gaatgaatta cgcacagttt ttgatctgta
ccagcataca aaatcatatg
3481 taactaactt aagtttaccc agatactcca atcccggata
atatctcccg acattgtgaa
3541 ccatctttat tttgtaccta actcttgttt ttagttacta
gccatatcgg cagccaatag
3601 gccacagagg ctgatttatg ttcatacccg agagccggtg
tcagtcaatt ggaaggacag
3661 atctgtgtca agctccacca ggtcaggtcc agagtccttc
gctatccaat taatttctag
3721 ctactctttt gtcaaatttt tgaatttcta aattttattc
tgcattgttt ttggactatt
3781 cgatgtgccg aaatttccat ggtggcgata aacagcttta
acagctattt gatttacttg
3841 tttgggtttt cggtctctgt ttttgagtac cactatgatc
tcactgagtt cccttactaa
3901 cttttccaaa tccactaacg tccatcctcg ttaatcgatc
gtaaatcttc atcaatgttt
3961 ctattcatcg acttgactga tcggctaagg atcggctcaa
gtcaacgtta atctaatcat
4021 gaagcccgca ttatgatcga actgaatcat gaatatcata
gaataacgtg agtactttag
4081 aacgcatgca ctcatcgctt gctgatacac atttggtgac
actattcaac attgtaagca
4141 aagcatatgt aaactacatt attgtatgat gatgtctact
catcatgccc tcttcataat
4201 tcgtttgtgc ttttgtagtt tctgtatcat ccacgattag
tatcttacat atatgcttaa
4261 atgaatccct tcaccattga ccaacaggag cgatctgctg
gccagcgtct gaacatcgtg
4321 attgtggccg agggagccat ggatcgcgag ggtcatccca
tcacagccga ggatgtaaag
4381 aaggtgatcg acgagcgtct gaagcacgat gcccgcatca
ctgtcttggg tcacgtgcag
4441 cgcggtggca atcccagtgc cttcgatcgt tttttggtaa
gtatttatac gaagatgacc
4501 tcaataactg tatttttagt aatttacaat gctagctagt
tttagattgt atcttttaaa
4561 ggttaatatt aaaacactaa agttaccaaa ataaggatat
acttattaag atatgattca
4621 atctattttt gacttgagag ttaatgcttc taccccgcat
aggcttgccg catgggagct
4681 gagccacttt ggcccttgat ggaggccacc aaggactcgg
tgccagtggt catctctctg
4741 gacggcaacc aggcggttcg cgtgccgctg atggagtgcg
tggagcgcac ccaggctggc
4801 aaggccatgg cggagaaacg ctgggcggat gccgtcaacg
tgcgcggacg ctccttcgag
4861 cggaatctgg agacctacaa gatgctgacg cgcttgaagc
cgcccaagga gaacttcgac
4921 gccgatggca agggaatcga aggataccgc ctagctgtga
tgcatattgg tgctccggct
4981 tgcggtatga atgccgctgt gcgcagcttc gtgcgcaatg
ccatctaccg cggtgatgtg
5041 gtttatggaa tcaacgacgg tgtcgagggt ctgattgcgc
gaaatgtccg cgagctgggc
5101 tggtaagtaa tccgacaagg gatcactcta aatcgtgtct
taatgcaatt caatttcagg
5161 tcggatgtct caggatgggt tggtcagggt ggcgcctact
tgggatccaa acgcacactg
5221 cctgagggca agttcaagga gatcgccgct cgtctcaagg
agtttaagat ccagggtctc
5281 ctgatcattg gtggctttga gagttaccat gccgccggac
agatcgccga tcagcggaca
5341 actacccaca gcttctgcat ccccattgtg gttattccat
cgacgatttc gaacaatgtg
5401 cccggcacag aattttctct gggatgcgac accggtttaa
atgagattac ggagatttgc
5461 gaccgtatcc gtcagtcggc acagggaacc aagcgccgag
tgttcgtcat tgagacgatg
5521 ggtggctact gtggctattt gccaccgttg gccggtttgg
ccggcggagc tgatgccgcc
5581 tacatctacg aggagaagtt ctccatcaag gagctgcagc
aggatgtcta ccacatgcct
5641 ccaagatggc cgaggcgtct cccgcggtct aatcctgcga
acgagaaggc tagcgagaac
5701 tacagcacgg acttcattta ccgcctgtac tcggaggagg
gcaagggcct cttcacctgc
5761 cgtatgaaca tcctgggtca catgcagcag ggcggctcac
ccactccctt cgaccgcaac
5821 atgggcacca aaatggccgc caagtgtgtc gactgctggc
cgcccagatc aaggcgaaca
5881 tcgacggcca acggtgtggt caactgcaag tccccggaca
ccgccacgct gctgggaatc
5941 gtgtcgcggc agtaccgctt ctcaccgctg gtcgacctca
ttgcagagac gaacttcgag
6001 taagtttaac ccgatggtca ccacaaaggg gactatgaat
tccctttaag ctcttggcat
6061 aacccactcc tcttccagtc aacgcatccc gaagaagcag
tggtggctgc gcctgcgtcc
6121 cctgctgcgc atcctggcca agcacgattc cgcctacgag
gaggagggta tgtacatcac
6181 cgtcgaggag gagtgtgaca ctgacgccgt cgcctaagcg
gactcggatc cggactctga
6241 tccccacttc cgcttatcac caccatcgct gcatgcaagc
atcaatgtcc acctatccgc
6301 accctcgaat cagtctctgt gttcgctttt taagttgttt
tggcaccgca tccgatccgt
6361 tttatggttt ttaccaagat cagacgaaga ggcccatgac
agcagcacat acgaaagtat
6421 ttcataatct acagacgcat aaaaaagcac aaatacataa
cttatttata ttgctcgcca
6481 gtcgattctt tcttatgatt tttccaaata tcgtgtattc
ttgaaatttg aatctcgaat
6541 actgaataac gttaatagtt cattctacat tgcagccgtt
ccgaagagtc tggcgaccta
6601 ttagcggaaa acgtttcgta tattatcgta attgttggtt
cttcagccca actaaattaa
6661 atgttaaagt atttagacaa tttacccagt caaaggaaca
aattctatct gcaaactgtt
6721 tttcgagtat tgttatcgaa cgaagcaatg atctatttgt
aacgacgtgc taaatacata
6781 tcaaacacct gttggcacaa tttatcgtcg tttgttttct
aattgttcag taggtatgtg
6841 ggaattgagc ttgaatttca cttttggtta agaaaacatt
tatttttgaa atggcttgct
6901 gggcgcgatt ggaacagcaa tgtttcagtg gaatcagcct
ataatacgat gtttctgcag
6961 caacttcact gcttgcaagc tatcggctca atcgattgtg
gtgccatcga tgcgcttcca
7021 gcctagacac ttgacttttt gcacggatgt gtatgtagtt
tattgtgctc gaaactgcgt
7081 aagtgatgtc agcgtgactg a