LOCUS RABCPK
1465 bp mRNA
MAM 30-MAR-1992
DEFINITION Rabbit muscle creatine phosphokinase (M isoenzyme)
mRNA, complete
cds.
ACCESSION K02831
NID g164897
KEYWORDS creatine phosphokinase; phosphoprotein.
SOURCE Rabbit muscle, cDNA to mRNA, clones
pCKM[15,19].
ORGANISM Oryctolagus cuniculus
Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
Vertebrata; Eutheria; Lagomorpha; Leporidae; Oryctolagus.
REFERENCE 1 (bases 1 to 1465)
AUTHORS Putney,S.D., Herlihy,W.C., Royal,N., Pang,H.,
Aposhian,H.V.,
Pickering,L., Belagaje,R.M., Biemann,K., Page,D., Kuby,S. and
Schimmel,P.R.
TITLE Rabbit muscle creatine phosphokinase:
cDNA cloning, primary
structure, and detection of human homologues
JOURNAL J. Biol. Chem. 259, 14317-14320 (1984)
MEDLINE 85054889
COMMENT [1] notes that the reactive cysteine
is located at position 282.
Clean copy sequence kindly provided by S.Putney, 28-MAY-1985.
FEATURES
Location/Qualifiers
source
1..1465
/organism="Oryctolagus cuniculus"
/db_xref="taxon:9986"
mRNA
1..1465
/note="CPK mRNA"
CDS
52..1197
/note="M creatine phosphokinase"
/codon_start=1
/db_xref="PID:g164898"
/translation="MPFGNTHNKYKLNYKSEEEYPDLSKHNNHMAKVLTPDLYKKLRD
KETPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYTVFKDLFDPIIQDRHGGFKP
TDKHKTDLNHENLKGGDDLDPHYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVE
ALNSLTGEFKGKYYPLKSMTEQEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIW
HNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNEH
LGYVLTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVF
DISNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK"
mat_peptide 55..1194
/note="M creatine phosphokinase mature peptide"
BASE COUNT 297 a 497
c 457 g 214 t
ORIGIN 445 bp upstream of MstI site.
1 acacgtagcc ccgcttcagc
acagcccagc cagccccccg acgccgccac catgccgttc
61 ggcaacaccc acaacaagta caagctgaac
tacaagtccg aggaggagta cccggacttg
121 agcaaacaca acaaccacat ggccaaggtg
ctgacccccg acctctacaa gaagctgcgc
181 gacaaggaga cgccctccgg cttcaccctg
gacgatgtca tccagacagg cgtggacaac
241 ccagggcacc ctttcatcat gaccgtgggc
tgcgtggccg gtgacgagga gtcctacacg
301 gtgttcaagg acctgttcga ccccatcatc
caggaccgcc acgggggctt caaacccacc
361 gacaagcaca agaccgacct caaccacgag
aacctcaaag gtggggacga cttggacccc
421 cactacgtgc tcagcagccg cgtgcgcacc
ggccgcagca tcaagggcta cacgctgccc
481 ccgcactgct cccgtggcga gcgccgggcc
gtggagaagc tctccgtgga agccctcaac
541 agcctgacgg gcgagttcaa ggggaagtac
taccccctga agagcatgac cgagcaggag
601 cagcagcagc tcatcgacga ccacttcctg
ttcgacaagc ccgtgtcccc gctgctgctg
661 gcctcgggga tggcccgcga ttggcccgac
gcccgcggta tctggcacaa cgacaacaag
721 agcttcctgg tgtgggtcaa cgaggaggac
cacctccggg tcatctccat ggagaagggc
781 ggcaacatga aggaggtctt ccgccgcttc
tgcgtggggc tgcagaagat tgaggagatc
841 tttaagaaag ctggccaccc cttcatgtgg
aatgagcacc tgggctacgt gctcacctgc
901 ccgtccaacc tgggcaccgg gctgcgtggg
ggcgtgcacg tgaagctggc gcacctgagc
961 aagcacccca agttcgagga gattctcacc
cgcctgcgcc tgcagaagcg gggcacaggg
1021 ggcgtggaca cggctgccgt gggctcggtg ttcgacattt
ccaacgccga ccggctgggc
1081 tcgtccgagg tcgagcaggt gcagctggtt gtggacggtg
tgaagctcat ggtggagatg
1141 gagaagaagc tggagaaagg ccagtccatc gacgacatga
tccccgccca gaagtaggcg
1201 cgcagcccgc cgccgccgcc gccgcctgga ggcgcccctc
gcccgcagcg cgcgcggccc
1261 cgggggctcg gagttccagc caatgggccc cgtcgtctgc
gtcctggcca atgaaatgcc
1321 tccctcacac ctcgctgtcc cggagctccg cccacctccg
gagcacctgc tgacggctgg
1381 cggggggggg ggctcggctc ccggcgtgtc tggagcgggg
gctttttccc cacgcaaagc
1441 agtgaataaa agcagcggcg gcctt
//