LOCUS HUMCKMT
6896 bp DNA
PRI 15-MAY-1996
DEFINITION Human mitochondrial creatine kinase (CKMT) gene, complete
cds.
ACCESSION J04469
NID g180589
KEYWORDS creatine kinase.
SOURCE human.
ORGANISM Homo sapiens
Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
Vertebrata; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6896)
AUTHORS Haas,R.C., Korenfeld,C., Zhang,Z.F., Perryman,B.,
Roman,D. and
Strauss,A.W.
TITLE Isolation and characterization
of the gene and cDNA encoding human
mitochondrial creatine kinase
JOURNAL J. Biol. Chem. 264 (5), 2890-2897 (1989)
MEDLINE 89123390
REMARK Erratum:[J Biol Chem 1989 Sep 25;264(27):16332]
COMMENT Computer-readable copy of sequence
for [1] kindly provided by
A.W.Strauss, 23-NOV-1988.
FEATURES
Location/Qualifiers
source
1..6896
/organism="Homo sapiens"
/db_xref="taxon:9606"
/clone="lambda-hCK39."
/dev_stage="adult"
/tissue_type="placenta"
/map="15"
exon
241..552
/gene="CKMT"
/number=1
gene
241..5570
/gene="CKMT"
sig_peptide 404..517
/gene="CKMT"
CDS
join(404..552,1157..1355,1559..1654,2041..2262,2392..2477,
2593..2716,4378..4512,5014..5139,5343..5459)
/gene="CKMT"
/EC_number="2.7.3.2"
/note="precursor"
/codon_start=1
/product="creatine kinase"
/db_xref="PID:g180590"
/translation="MAGPFSRLLSARPGLRLLALAGAGSLAAGFLLRPEPVRAASERR
RLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFI
KTVGMVAGDEETYEVFADLFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVL
SSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQ
QLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKG
GNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPL
LSKDSRFPKILENLRLQKRGTGGVDTAATGGVFDISNLDRLGKSEVELVQLVIDGVNY
LIDCERRLERGQDIRIPTPVIHTKH"
mat_peptide join(518..552,1157..1355,1559..1654,2041..2262,2392..2477,
2593..2716,4378..4512,5014..5139,5343..5456)
/gene="CKMT"
/EC_number="2.7.3.2"
/product="creatine kinase"
intron
553..1156
/gene="CKMT"
/number=1
exon
1157..1355
/gene="CKMT"
/number=2
intron
1356..1558
/gene="CKMT"
/number=2
exon
1559..1654
/gene="CKMT"
/number=3
intron
1655..2040
/gene="CKMT"
/number=3
exon
2041..2262
/gene="CKMT"
/number=4
intron
2263..2391
/gene="CKMT"
/number=4
exon
2392..2477
/gene="CKMT"
/number=5
intron
2478..2592
/gene="CKMT"
/number=5
exon
2593..2716
/gene="CKMT"
/number=6
intron
2717..4377
/gene="CKMT"
/number=6
exon
4378..4512
/gene="CKMT"
/number=7
intron
4513..5013
/gene="CKMT"
/number=7
exon
5014..5139
/gene="CKMT"
/number=8
intron
5140..5342
/gene="CKMT"
/number=8
exon
5343..5570
/gene="CKMT"
/number=9
BASE COUNT 1746 a 1718 c
1720 g 1712 t
ORIGIN 227 bp upstream of PstI site.
1 catgccacat ccccggggcg
ggagggggct acatccccgg ctttagacgc gcgagtctca
61 ggtcccgcta attacctggc gggtgctgcc
cacccctgcc ctcgcgcacc tagcgcgtgg
121 cagcgggaag gcggggcctg ggggagcccc
acccctggag actgcggctg gggcctccct
181 ctcctccgcc cgcccgcctg ccactagctc
attgcgcctc tcctgcagtc tgattgggca
241 ccggctccca ttccggctcc agcctccaat
ccgaccccca tttcggctgc agcctcggac
301 ctagctccgg ccctcggtct atccggttgc
atcctccctc cctgttccgg atcttatctt
361 gcgccagcgc ctactccagg atcccgtagc
cagacctcaa gccatggctg gtcccttctc
421 ccgtctgctg tccgcccgcc cgggactcag
gctcctggct ttggccggag cggggtctct
481 agccgctggg tttctgctcc gaccggaacc
tgtacgagct gccagtgaac gacggaggct
541 gtatcccccg aggtaacagt gcctgaggcg
cgggaggagg cgggggcagg aggtgatggg
601 aacgaaggtg cgggtagaag tgagaatccg
ggcaacagag aagggctata atcacgaagg
661 ccctggagct ggagggctgt gcagtctgca
gacctcagtg gggtgggggt gggggccaaa
721 accataaagc aagaacattc ctggggacct
gccaagacca gctctggccc tacgagttct
781 agctgcactg gctgcccaaa tccctaattg
taaagccagg aactatcctt ttcgctcccc
841 tccatctcct tccctcattt cctcaattcc
tctccttagg cttttcccct cctccatccg
901 tagtgttgtg tcatgggagg aaagaactga
gcagatctga agaaactgag ctggccagcc
961 agaggcaact agaactatta ggaaagcata
gactctgaaa gtccctaaag agattaccaa
1021 ggtttaccct ctttctaatt cccctcctcc cgcggagcaa
agccagacat ggccaactgg
1081 acagctccca ggtaactgca ctaggtctag gcgtctgtga
ccctccctcc atggttactg
1141 ggtaccccct ccccagcgct gagtacccag acctccgaaa
gcacaacaac tgcatggcca
1201 gtcacctgac cccagcagtc tatgcacggc tctgcgacaa
gaccacaccc actggttgga
1261 cgctagatca gtgtatccag actggcgtgg acaaccctgg
ccaccccttc atcaagactg
1321 tgggcatggt ggctggagat gaggagacct atgaggtagg
gggtccccag agtctccctg
1381 atgatccaat tcatcttccc agtaatccca gctcctttcc
cttaaagacc tctcactttc
1441 ccccaagact ctgagccccc catacttaag ttttctgaac
cagtgaaatc aatgcacaat
1501 tgaagtctgg ggagggattc cctctcctta accatctctc
cctcttaact ccccttaggt
1561 atttgctgac ctgtttgacc ctgtgatcca agagcgacac
aatggatatg acccccggac
1621 aatgaagcac accacggatc tagatgccag taaagtgagt
tcaaatatcc cacttctgat
1681 ttgcattgcc tgtgtacaac actctgtatc tccaacccct
tcaccttatt tcctgactca
1741 tggtcattat actgctgagc ttttaatctt aatgtaagga
aagaatcata tcttaagggg
1801 cagcatatat ggagatggaa ggatagataa gaatgaccat
gacccaaggt gggtggtttg
1861 gggacgggtc tgcaatgccc ccttcaattc cagtgctttc
ccaaagggcc tcttcttcca
1921 atgcatgcag gaagaatgca cacagagtcc tctaatgcct
aaggaaggtc tctcctttcc
1981 caggggccct cagttcccac cgtgtttctg tgacttacat
tcatttccct tatctcccag
2041 atccgttctg gctactttga tgagaggtat gtattgtcct
ctagagtcag aactggccga
2101 agcatccgag gactcagtct gcctccagct tgcactcgag
cagagcgacg agaggtggaa
2161 cgtgttgtgg tggatgcact gagtggcctg aagggtgacc
tggctggacg ttactatagg
2221 ctcagtgaga tgacagaggc tgaacagcag cagcttattg
atgtgagggc cttaagaggg
2281 tgctggttgg tgggagcaga tggggaaggc tgggccagat
gagacatggg ctctgaaagg
2341 cccaggggcc accatgaaga ttcttaaccc aagtcccgtt
actcttccca ggaccacttt
2401 ctgtttgata agcctgtgtc cccgttgctg actgcagcag
gaatggctcg agactggcca
2461 gatgctcgtg gaatttggta tgaagctgct cattacctct
tttgtcttca tgccctcata
2521 aatgcttttt ttccctctat ctctcccaat tcttgccttg
cctcttgatc actgtccctc
2581 tccggccctc aggcacaaca atgagaagag cttcctgatc
tgggtgaatg aggaggatca
2641 tacacgggtg atctccatgg agaagggtgg taacatgaag
agagtgtttg aaagattctg
2701 ccgaggcctc aaagaggtta gagaagacta tgtaggggag
ctaggtggga ggacataagg
2761 aaaaccaaag agtagcataa atagattatg taatttacca
accaacccag gacatgtctt
2821 atagtaaaaa ggactatcta ggactcactc caggactaaa
ggtgtaaacc agctgggacc
2881 atactgggaa aaccaggaca tgtggtcaca ctaagattag
gaaaagaaag agtgtcagga
2941 atcttaggaa gtgaacaagg cttttgacag agagtgcaaa
gaaggaataa atgagatggc
3001 acgtcagtgc ctgggatgtg tgcagtggga tggtgaggtg
tgcagataag gaaaacattc
3061 gagcttagat tgatgttggc ggggagaggt tgctgtgttc
atgactctaa tataaccacc
3121 cagttctgag acaaggtagg ccttgactct ggattctatc
attcttgtta aagtttcggg
3181 tctaggcttt aagttgagag ttcggagaga gactggggaa
ggtggaggat agaatggttc
3241 gagttctaga atatgtggct ctagatgaga ggttgaactg
aatcatcaat cctacatgga
3301 ttgggtctcc gtattcaagt ctacattaga aatccccata
aactcaattc aattcttact
3361 gtatgttctc aaacatacag ttctatttta ggtttgcaaa
gaaaaagagc tcctctttta
3421 gattctgaga agtttctact atttttggca agtaatagat
aacatattct gactatgagt
3481 gggtagggaa gtacctttaa attatatgcc tcagtttcct
catctgtaaa attgggataa
3541 tgagattttc tacattttag gttgttgtgg ggattaagtg
aaatacaggt aaagtacttg
3601 gtccacagta agtgcttaat aagtgttaaa gtgttagctg
caatattatt ctggatggaa
3661 gagtttcccc ccatgttcag catgtaagat atcccctatg
gcatggttcc ttctgaacta
3721 taaagaggat ccctttactc atgttgggtt gtggtctttg
tgaccatcat tctgctagat
3781 cccttgtctc ttgaactcta atagtcatct tcatgactac
atggttaagt gaagccaaac
3841 gccttccccc cgccccctat tcctatgaat ctggcttttc
tgctctgttt tcatctttct
3901 ctgcattcac acaggtgctc cgttcacagc taacagaatg
ttatcttacc tcttcctggc
3961 aaagcttaca ccttcatctt ctgtctgaag ggacccttct
aagctctagg ctcattagca
4021 aagcaaagat aatcgatgca tgcagacctc attgaataat
cagtcatctc tcagttcagt
4081 ttaccacctc tgttcatttc cctagatcat ccttaataca
ccactccttc gagttttctt
4141 cttccacata agatattttt tcacaatctc attattatgc
acatcataat tttgcatcat
4201 gcatgcatga aaacaataac aaaccttttt catttaaaaa
aagaccaatg tcattcattc
4261 acagccaagt ttctgttcta gacatatttc tagtgttctt
gtgggtctag ctaagggagg
4321 gtccagggtt aatgaaatat ccctgatttt tcgttaacaa
aacctttgtg gactcaggtg
4381 gagagactta tccaagaacg tggctgggag ttcatgtgga
atgagcgttt gggatacatc
4441 ttgacctgtc catctaacct gggcactgga cttcgggcag
gagtgcacat caaactgccc
4501 ctgctaagca aagtaaagga gttgtggggt tacagagggg
tgtgagtaag gaagggtggg
4561 ttgtggatgg ggagggagtg gaccctttgg aaaggagcca
aacatgttgt ggctaaaggg
4621 tcagaggaca ggccaggcac agtggctcat gcctctaatc
ccaacacttg ggaggccaag
4681 gcaggcagat tacttgagcc caggagttca agaccagcct
gggcaacctg gtgaaacccc
4741 atctctacct acaaatacaa aagttagctg ggtgtagtgg
aggctgaggt gagaggatca
4801 cttaagcctg ggaagtcgag gcttcagtga gctgtgatca
ctccagcctg ggtgacagag
4861 agagaccctg tctaaaaaaa attaaaaaag aaaaaagaaa
aaaggaaaaa aaaagttcag
4921 gagacagagc tctgagcagg ttcagggctc tttcaggtag
gacctagtct ctgcctctat
4981 tgaccctgct cccaatccct atctcctctc taggatagcc
gcttcccaaa gatcctggag
5041 aacctaagac tccaaaaacg tggtactgga ggagtggaca
ctgctgctac aggcggtgtc
5101 tttgatattt ctaatttgga ccgactaggc aaatcagagg
tgagatccta agggattagg
5161 acaaggagag gtataggtct gcgagggccg aaatatggca
gtgagtgagc ctccgggatg
5221 taacataatc tgaaatgaaa ttcaggttga gtgggaggca
attggaaatg agcaggcaag
5281 tcagtcagtg ataaagaaaa actcagactg taggaagcag
atcaaagatt agtgtccctt
5341 aggtggagct ggtgcaactg gtcatcgatg gagtaaacta
tttgattgat tgtgaacggc
5401 gtctggagag aggccaggat atccgcatcc ccacacctgt
catccacacc aagcattaac
5461 tccccatcgc cagctgatga ctcaagattc ccaggagttt
tgctcattct aatgatggcc
5521 cattctactt gctctggacc tgcccccgca tcccctgcct
ccatcctagt aaagactcct
5581 tgctatgctg cagctgtctg tgttacttct aatggtgggg
tgaggaggga gcagccttca
5641 ggaaatgaaa agaggcagtg ggattattta tgatggaaag
agactccaga tatggcaacc
5701 caggaacact gattctcagg tgggtggaaa gcattaacat
tttacccata ttcctcatca
5761 gcttctgaaa ataatcagga tgcacttctg tttgcacttt
attcattatg acttaagatt
5821 tctctcccca caatctcctt ctactgtaga gacaggctca
tagcaggtgg ccaaggaagc
5881 tgatagtcaa taccagggac caggaaggtc gtgaccagtc
ctggaggccc caggctgtac
5941 ttcgacctat aatagacagg gaatgggagt aatatcacaa
ctcagctctc caggagcatt
6001 gatacttgga aattagcgct ctgcctgtag actccttcac
tccagggatc tccctgggtg
6061 cactctaaga gccagacagc accaaattag gggtttgatt
ctgggtcagg agatggagga
6121 tcaagctgtg cagctgggaa ctcaccttgc tgttctgggc
tctcctttcc ctcatgttgg
6181 gcccatgcaa ctgctcgtcg ctgctcagga ctcagaaagg
ccatttgctc aggagtgaca
6241 gccacagcct gagcactggt gagactagat agttggatgg
gactaaacac cacctgaggg
6301 caggggtagg aatcagtgca tgcatgtagt ccccattggg
ccctggctct cctgtggtca
6361 ccccagtcca ttaatactta cagcaaattt aggaggaggg
atgacagaaa tggcaagagg
6421 agtaacgccc tggatctgtc cccgcagcag tgctgaaaga
gccaggtctg ggatcccagc
6481 tgttgaagca agtggcatcc aaacattgtc ttagactgac
cttccctctc ttcaaaccta
6541 tagaccttct ctaactactc ccaaagtgcc ctatcataga
ccttccccaa tatgtctcta
6601 gccccttatt taaacaccct caggccccca ccttaagaat
tgcagggcag tcttccatcc
6661 agtccaccca tggtatagaa accaaaccaa cttgcaccag
cagtggccca gctccccacc
6721 tgctatggtg ccaatttcag tgaagatctc aggcccccag
ttactgattg ggccaaaccc
6781 accaggcagt acaagtaggt gggccagaac ctccagttgt
tcctcagagc actgcagatg
6841 cagggtgccg aggaagagag ctgcttggct gtagaacagt
gggaaggaag gaagaa
//