LOCUS DROADH 2126 bp DNA INV 18-SEP-1997

DEFINITION D.melanogaster alcohol dehydrogenase gene, complete cds.

ACCESSION J01066 M11290

NID g156787

KEYWORDS alcohol dehydrogenase.

SOURCE D.melanogaster, cDNA to mRNA, and DNA (see comment).

ORGANISM Drosophila melanogaster

Eukaryotae; mitochondrial eukaryotes; Metazoa; Arthropoda;

Tracheata; Insecta; Pterygota; Diptera; Brachycera; Muscomorpha;

Ephydroidea; Drosophilidae; Drosophila.

REFERENCE 1 (bases 1683 to 2126; 1528 to 1612)

AUTHORS Benyajati,C., Wang,N., Reddy,A., Weinberg,E. and Sofer,W.

TITLE Alcohol dehydrogenase in Drosophila: Isolation and characterization

of messenger RNA and cDNA clone

JOURNAL Nucleic Acids Res. 8, 5649-5667 (1980)

MEDLINE 81124290

REFERENCE 2 (bases 1433 to 1577)

AUTHORS Goldberg,D.A.

TITLE Isolation and partial characterization of the Drosophila alcohol

dehydrogenase gene

JOURNAL Proc. Natl. Acad. Sci. U.S.A. 77, 5794-5798 (1980)

MEDLINE 81077261

REFERENCE 3 (bases 1044 to 1158; 1192 to 1949)

AUTHORS Benyajati,C., Place,A.R., Powers,D.A. and Sofer,W.

TITLE Alcohol dehydrogenase gene of Drosophila melanogaster: Relationship

of intervening sequences to functional domains in the protein

JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78, 2717-2721 (1981)

MEDLINE 81247357

REFERENCE 4 (bases 1137 to 1213)

AUTHORS Benyajati,C., Place,A.R., Wang,N., Pentz,E. and Sofer,W.

TITLE Deletions at intervening sequence splice sites in the alcohol

dehydrogenase gene of Drosophila

JOURNAL Nucleic Acids Res. 10, 7261-7272 (1982)

MEDLINE 83116953

REFERENCE 5 (bases 1 to 1100)

AUTHORS Benyajati,C., Spoerel,N., Haymerle,H. and Ashburner,M.

TITLE The messenger RNA for alcohol dehydrogenase in Drosophila

melanogaster differs in its 5' end in different developmental

stages

JOURNAL Cell 33, 125-133 (1983)

MEDLINE 84282660

REFERENCE 6 (bases 1122 to 1216; 1291 to 1321; 1890 to 1949)

AUTHORS Benyajati,C., Place,A.R. and Sofer,W.

TITLE Formaldehyde mutagenesis in Drosophila: Molecular analysis of

Adh-negative mutants

JOURNAL Mutat. Res. 111, 1-7 (1983)

MEDLINE 84013866

FEATURES Location/Qualifiers

source 1..2126

/organism="Drosophila melanogaster"

/db_xref="taxon:7227"

gene 1..2126

/note="Alcohol dehydrogenase"

/gene="Adh"

/allele=""

/db_xref="FlyBase:FBgn0000055;"

prim_transcript 267..2126

/gene="Adh"

/note="Adh adult mRNA [1],[5]"

intron 354..1007

/gene="Adh"

/note="Adh adult 5' intron"

/db_xref="FlyBase:FBgn0000055;"

prim_transcript 974..2126

/gene="Adh"

/note="Adh larval mRNA [5]"

CDS join(1044..1142,1208..1612,1683..1949)

/gene="Adh"

/note="alcohol dehydrogenase"

/codon_start=1

/db_xref="FlyBase:FBgn0000055;"

/db_xref="PID:g156788"

/translation="MSFTLTNKNVIFVAGLGGIGLDTSKELLKRDLKNLVILDRIE
NPAAIAELKAINPKVTVTFYPYDVTVPIAETTKLLKTIFAQLKTVDVLIN
GAGILDDHQIERTIAVNYTGLVNTTTAILDFWDKRKGGPGGIICNIGSVT
GFNAIYQVPVYSGTKAAVVNFTSSLAKLAPITGVTAYTVNPGITRTTLV
HKFNSWLDVEPQVAEKLLAHPTQPSLACAENFVKAIELNQNGAIWKL
DLGTLEAIQWTKHWDSGI"

BASE COUNT 623 a 526 c 448 g 529 t

ORIGIN HinfI site [Cell 33, 125-133 (1983)].

1 gactcttttt tgattttgga atattttcgt tcgttttatg tttttacgtt ttcgcatatt

61 tgtttcacag tgcactttct ggtgttccat tttctattgg gctcttaacc ccgcatttgt

121 ttgcagatca cttgcttgcg catttttatt gcattttaca tattacacat tatttgaacg

181 ccgctgctgc tgcatccgtc gacgtcgact gcactcgccc ccacgagaga acagtattta

241 aggagctgcg aaggtccaag tcaccgatta ttgtctcagt gcagttgtca gttgcagttc

301 agcagacggg ctaacgagta cttgcatctc ttcaaattta cttaattgat caagtaagta

361 gcaaaagggc acccaattaa aggaaattct tgtttaattg aatttattat gcaagtgcgg

421 aaataaaatg acagtattaa ttagtaaata ttttgtaaaa tcatatataa tcaaatttat

481 tcaatcagaa ctaattcaag ctgtcacaag tagtgcgaac tcaattaatt ggcatcgaat

541 taaaatttgg aggcctgtgc cgcatattcg tcttggaaaa tcacctgtta gttaacttct

601 aaaaatagga attttaacat aactcgtccc tgttaatcgg cgccgtgcct tcgttagcta

661 tctcaaaagc gagcgcgtgc agacgagcag taattttcca agcatcaggc atagttgggc

721 ataaattata aacatacaaa ccgaatacta atatagaaaa agctttgccg gtacaaaatc

781 ccaaacaaaa acaaaccgtg tgtgccgaaa aataaaaata aaccataaac taggcagcgc

841 tgccgtcgcc ggctgagcag cctgcgtaca tagccgagat cgcgtaacgg tagataatga

901 aaagctctac gtaaccgaag cttctgctgt acggatcttc ctataaatac ggggccgaca

961 cgaactggaa accaacaact aacggagccc tcttccaatt gaaacagatc gaaagagcct

1021 gctaaagcaa aaaagaagtc accatgtcgt ttactttgac caacaagaac gtgattttcg

1081 ttgccggtct gggaggcatt ggtctggaca ccagcaagga gctgctcaag cgcgatctga

1141 aggtaactat gcgatgccca caggctccat gcagcgatgg aggttaatct cgtgtattca

1201 atcctagaac ctggtgatcc tcgaccgcat tgagaacccg gctgccattg ccgagctgaa

1261 ggcaatcaat ccaaaggtga ccgtcacctt ctacccctat gatgtgaccg tgcccattgc

1321 cgagaccacc aagctgctga agaccatctt cgcccagctg aagaccgtcg atgtcctgat

1381 caacggagct ggtatcctgg acgatcacca gatcgagcgc accattgccg tcaactacac

1441 tggcctggtc aacaccacga cggccattct ggacttctgg gacaagcgca agggcggtcc

1501 cggtggtatc atctgcaaca ttggatccgt cactggattc aatgccatct accaggtgcc

1561 cgtctactcc ggcaccaagg ccgccgtggt caacttcacc agctccctgg cggtaagttg

1621 atcaaaggaa acgcaaagtt ttcaagaaaa aacaaaacta atttgattta taacaccttt

1681 agaaactggc ccccattacc ggcgtgaccg cttacaccgt gaaccccggc atcacccgca

1741 ccaccctggt gcacaagttc aactcctggt tggatgttga gccccaggtt gctgagaagc

1801 tcctggctca tcccacccag ccatcgttgg cctgcgccga gaacttcgtc aaggctatcg

1861 aactgaacca gaacggagcc atctggaaac tggacctggg caccctggag gccatccagt

1921 ggaccaagca ctgggactcc ggcatctaag aagtgatact cccaaaaaaa aaaaaaaaca

1981 taacattagt tcatagggtt ctgcgaacca gaagatattc acgcaaggca ataaggctga

2041 ttcgatgcac actcacattc ttctcctaat acgataataa aactttccat gaaaaatatg

2101 gaaaatatat gaaaattgag aaatcc