LOCUS       ECOADD       1200 bp    DNA             BCT       13-APR-1992
DEFINITION  E.coli adenosine deaminase (add) gene, complete cds.
ACCESSION   M59033 J05320
NID         g145200
KEYWORDS    adenosine deaminase.
SOURCE      E.coli K-12 DNA.
  ORGANISM  Escherichia coli
            Eubacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Chang,Z., Nygaard,P., Chinault,A.C. and Kellems,R.E.
  TITLE     Deduced amino acid sequence of Escherichia coli adenosine deaminase
            reveals evolutionarily conserved amino acid residues: Implications
            for catalytic function
  JOURNAL   Biochemistry 30, 2273-2280 (1991)
  MEDLINE   91152042
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Escherichia coli"
                     /strain="K-12"
                     /db_xref="taxon:562"
     gene            49..1139
                     /gene="add"
     -35_signal      49..54
                     /gene="add"
                     /note="consensus; putative"
     -10_signal      67..72
                     /gene="add"
                     /note="consensus; putative"
     misc_feature    67..88
                     /gene="add"
                     /note="operator"
     mRNA            78..1116
                     /gene="add"
                     /product="adenosine deaminase"
     mRNA            80..1116
                     /gene="add"
                     /product="adenosine deaminase"
     RBS             99..103
                     /gene="add"
     CDS             109..1107
                     /gene="add"
                     /EC_number="3.5.4.4"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /db_xref="PID:g145201"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFEAACQQELEAFLAHRD
                     QITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAERI
                     GHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASINT
                     DDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     terminator      1116..1139
                     /gene="add"
BASE COUNT      282 a    321 c    305 g    292 t
ORIGIN
        1 cgtcgttaac cccaattcgc aagtaaaaaa tcgttgcaat cgtggatttt taccctgctt
       61 tgtttttata atggtgcgca cttttatatc cagaaaaaga gtgcgaccat gattgatacc
      121 accctgccat taactgatat ccatcgccac cttgatggca acattcgtcc ccagaccatt
      181 cttgaacttg gccgccagta taatatctcg cttcctgcac aatccctgga aacactgatt
      241 ccccacgttc aggtcattgc caacgaaccc gatctggtga gctttctgac caaacttgac
      301 tggggcgtta aagttctcgc ctctcttgat gcctgtcgcc gcgtggcatt tgaaaacatt
      361 gaagatgcag cccgtcacgg cctgcactat gtcgagctgc gtttttcacc aggctacatg
      421 gcaatggcac atcagctgcc tgtagcgggt gttgtcgaag cggtgatcga tggcgtacgt
      481 gaaggttgcc gcacctttgg tgtgcaggcg aagcttatcg gcattatgag ccggaccttc
      541 gaagccgcct gtcagcaaga gctggaggcc tttttagccc accgtgacca gattaccgca
      601 cttgatttag ccggtgatga acttggtttc ccgggaagtc tgttcctttc tcacttcaac
      661 cgcgcgcgtg atgcgggctg gcatattacc gtccatgcag gcgaagctgc cgggccggaa
      721 agcatctggc aggcgattcg tgaactgggt gcggagcgta ttggacatgg cgtaaaagcc
      781 attgaagatc gggcgctgat ggattttctc gccgagcaac aaattggtat tgaatcctgt
      841 ctgacctcca atattcagac cagcaccgta gcagagctgg ctgcacatcc gctgaaaacg
      901 ttccttgagc atggcattcg tgccagcatt aacactgacg atcccggcgt acagggagtg
      961 gatatcattc acgaatatac cgttgccgcg ccagctgctg ggttatcccg cgagcaaatc
     1021 cgccaggcac agattaatgg tctggaaatg gctttcctca gcgctgagga aaaacgcgca
     1081 ctgcgagaaa aagtcgccgc gaagtaacaa aatggatggt gcaaatgcac accatttttc
     1141 atgcaaggca caaagtcgcg cgatgtttcg gcggattcga tgcccagctc aatcaactcc
//