This page will summarize the results of our efforts to clone all 5 yeast IDH-encoding genes.
These are the gels from Tuesday's results. All gels are 1% agarose. I want to verify which samples are in which lanes. Please tell me where your samples are: gel, left or right rows, lane # with #1 on the bottom and# 8 at the top. Also, tell me which enzyme(s) you used.
Still need info about IDP1, IDP2 and what enzyme was used on IDH1.
Gel A
Left: Lanes 1-4 are IDP1 cut with BglII and 7
& 8 are IDP3 cut with ClaI and HnidIII. MW in lane 5.
Right: Lanes 1-6 are IDP3 cut with ClaI and HnidIII. MW in lane 8.
Gel B
Left: lanes 1-4 and 6-7are IDH2 cut with EcoRV.
MW in lane 5.
Right: Lane 1, 5 and 6 are IDH2 cut with EcoRV. IDH1 cut with SalI in lanes
3 and 4. MW in lane 2.
Gel C
Left: Lanes 1-4 and 6-8 IDP2 cut with BglII MW
in lane 5.
Right: Lane 1 IDP2 cut with BglII. Lanes 2-4 are IDH1 cut with SalI. MW in lane
5.
Gel D = Tia and Lisa's recut.
Lisa and Tia recut their IDP3 containing plasmids
with ClaI and HindIII. Lisa's are on the left and Tia's on the right.
Uncut plasmids in lanes 6-8. MW in lanes 5 for both gels.
Lab Schedule In Context of Research Project
