1) Watch Lab safety video
2) Practice pipetting (see reminder page).
3) Make solutions. What is 1X anyway?
If a graduated cylinder was used for a solution of salts, just rinse it 3 times in distilled water before using. Cylinders that were used with a protein solutions (e.g. media) should be washed with detergent before using again.
sterile yellow and blue tips | |
sterile culture tubes | |
sterile tooth picks | |
LB + amp. agar plates | make 1 L |
5M NaCl | make 100 mL |
1 M Tris (pH 8.0) | make 1 L |
0.5 M EDTA | make 500 mL |
5 M NaOH | make 100 mL |
10X tris saline | make 125 mL |
10X transfer buffer (western) | make 500 mL |
20X SSC | make 1 L |
ECL 2° Wash | make 2 L |
Ampicillin stock | ask Dr. C. |
5X TBE | make 1 L |
Reagent |
Volume |
Container |
Autoclave? |
sterile yellow tips | 1 box per person | yellow box | YES |
sterile culture tubes | 1 full blue rack | blue rack | YES |
sterile tooth picks | 1 beaker per lab day | small beaker + foil | YES |
LB+amp. agar plates |
1 L
|
2 L Flask | YES + amp and pour |
0.5 M EDTA | 500 mL | 1 L bottle | NO |
5M NaCl | 100 mL | 100 mL bottle | NO |
5 M NaOH | 100 mL | 100 mL bottle | NO |
10X tris saline | 125 mL | 250 mL bottle | NO |
Turbo Denature Buff. | 2 L | 2 x 1 L bottles | NO |
10X transfer stock (western) | 500 mL | 500 mL bottle | NO |
20X SSC | 1L | 1L bottle | NO |
ECL 2° Wash | 2 L | 2 x 1L bottle | NO |
Ampicillin Stock | ask Dr. C. | blue cap 15 mL tube | filter sterilize |
5X TBE | 1 L | 1 L bottle | NO |
sterile yellow tips | |
sterile culture tubes | |
sterile tooth pick | |
LB liq. medium | make 500 mL |
LB + amp agar plates | make 1L |
3 M sodium acetate | make 50 ml you figure this one out (pH to 5.2 with glacial acetic acid) |
Turbo Transfer Buffer | make 2 L |
Turbo Denature Buffer | make 2 L |
Turbo 5X Neutralizing Buffer | make 500 mL |
ECL primary wash (w/o urea) | make 2 L |
ponsceau | make 100 mL |
1X stock of TE | 50 mL |
5X TBE | make 1 L |
Reagent |
Volume |
Container |
Autoclave? |
sterile yellow tips | 1 box per person | yellow box | YES |
sterile culture tubes | 1 full blue rack | blue rack | YES |
sterile tooth picks | 1 beaker | small beaker + foil | YES |
LB media, no amp | 500 ml | five 100 ml bottles | YES |
LB+amp. agar plates | 1L | in a two liter flask | YES + amp and pour |
3 M sodium acetate | 50 ml | 100 ml bottle | NO pH = 5.2: glacial acetic |
Turbo Denature Buff. | 2 L | 2 x 1 L bottles | NO |
Turbo Trans. Buf. | 2 L | 2 X 1 L bottle | NO |
Turbo Neut. Buf. | 200 ml | 500 ml bottle | NO |
ECL 1° wash | 2 L | 2 X 1 L bottle | NO |
1X stock of TE | 50 mL | 100 mL bottle | YES |
ponsceau | 100 ml | 100 ml bottle | NO |
5X TBE | 1 L | 1 L bottle | YES |
Exposures
Clothing
Mental Preparation
Other Points
Go To Lab Schedule In Context of Research Project