*permission to use image requested from Proceedings of the National Academy of Sciences .
The Gene
The Popular Press
The Science Behind The Gene
Figure 1.
*permission to use image requested
from Proceedings of the National Academy of
Sciences
.
Table 1 (below) shows the results of the fine mapping around the D4S1572 marker, with the position of the marker tested, the MLS, and the maximum Heterogeneity Logarithm of Odds (hlod) scores shown for each marker. The hlod test is very similar to the test that yields an MLS, and the scores may be evaluated in a similar manner. In Table 1, the MLS and hlod scores both peak at the D4S1564 marker, identifying this marker as the locus of maximum correlation.
Table 1. Nonparametric MLS and
parametric hlod scores within the exceptional longevity susceptibility locus
relative to
markers in
the chromosome 4 region
--------------------------------------------------------------------------------
Marker
Position
MLS* hlod*
dom
D4S1534
95.0
0.57
0.54
D4S414
100.8
1.53
1.30
D4S2986
105.3
2.78
2.26
D4S1572
108.0
3.07
2.57
D4S411
109.0
3.07
2.60
D4S1564
112.6
3.65
3.26
D4S406
117.1
2.55
2.15
D4S1611
121.6
1.70
1.56
D4S402
124.5
1.39
1.06
D4S2975
126.7
0.94
0.89
--------------------------------------------------------------------------------
* Nonparametric
MLS and parametric hlod scores were calculated for within the exceptional
longevity susceptibility
locus relative
to markers in the chromosome 4 region. Positions are from the
Marshfield map
. Fine mapping at
an average
of 1 marker every 3 cM around the peak noted in Fig. 1 resulted in an increased
MLS (3.65) at
marker D4S1564.
A dropoff of 1.5 in the MLS score on either side of the peak MLS defines
the area in which we can
be 95% confident
the gene resides. A dropoff in the MLS of 2 on either side of the peak is
observed in a 20-cM
region encompassed
by D4S414 and D4S1611. The MLS scores in this region and the hlod scores
under the
dominant
model are shown.
*all data in blue borrowed from Proceedings
of the National Academy of Sciences
, permission requested.
The data for the D4S1564 marker is shown below:
LOCUS HS248ZG9 331 bp DNA PRI 28-NOV-1994
DEFINITION H. sapiens (D4S1564) DNA segment containing (CA) repeat; clone
AFM248zg9; single read.
ACCESSION Z23817
VERSION Z23817.1 GI:394017
KEYWORDS CA repeat; dinucleotide repeat; GT repeat; microsatellite DNA;
microsatellite marker; repeat polymorphism.
SOURCE human.
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 331)
AUTHORS Weissenbach,J.
TITLE Direct Submission
JOURNAL Submitted (12-JUL-1993) Genethon, B.P. 60, 91002 Evry Cedex France.
E-mail: Jean.Weissenbach@genethon.fr
REFERENCE 2 (bases 1 to 331)
AUTHORS Gyapay,G., Morissette,J., Vignal,A., Dib,C., Fizames,C.,
Millasseau,P., Marc,S., Bernardi,G., Lathrop,M. and Weissenbach,J.
TITLE The 1993-94 Genethon human genetic linkage map
JOURNAL Nat. Genet. 7 (2 Spec No), 246-339 (1994)
MEDLINE 95004593
COMMENT cloning vector is M13mp18ASBB;
full automatic.
FEATURES Location/Qualifiers
source 1..331
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="4"
/cell_line="CEPH 134702"
/clone_lib="genomic DNA"
BASE COUNT 92 a 81 c 49 g 99 t 10 others
ORIGIN
1 agctactnag naggctgggg caggaaanta anttcagccc aggaggtgaa ggttgcagtn
61 agccgagatc acgccactgc actccagcct gggcaacana ntganacacc tctttctttn
121 tntctctctc tctctctctc tctttcacac acacacacac acacacacac acaatgaaaa
181 aaagtcattt tcctatagaa tgctctccag ttactgataa agaatactcc cctttctcaa
241 tgtttcctag aaatctcatt tgagtttaac aaagttcaat ttaagaagtt tgatcacatt
301 taatgttttt atttcctttg ccacggtttt t
*data in purple borrowed from BLAST search on National Center for Biotechnology Information
database online, results from Genbank. Permission requested to use this data.
Conclusions
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pelowry@davidson.edu
Davidson College