There are several experiments that I would like to see done for Ady2p. First, no information was found on the Y2H database. I think it would be beneficial to use the yeast two-hybrid method, with Ady2p as the bait protein, to see what other proteins interact with it. I predict we would find other proteins involved in ion transport and DNA metabolism would interact with Ady2p, and we could gain insight into the the exact mechanisms of these processes in yeast.

A second experiment that I would perform is to create a knockout strain of this gene and study what effects that has on the organism. Studying the resulting phenotype would provide us with further insight into the role Ady2p plays in the biological processes it is associated with. We also learned from the SGD that an ADY2 mutant is viable, but perhaps if it is tested in different media, it would be affected differently.

It would also be interesting to see a protein microarray, through the use of antibodies, to detect the amount of Ady2p in yeast populations grown in different media. Specifically, it would be beneficial to study populations grown in different nitrogen and ammonia medias, since ADY2 is known to regulate nitrogen utilization and ammonia production.

Finally, the structure of this protein needs to be determined. Using the protein sequence, we can predict the 3D structure of this protein, and in this way visualize any possible interactions it has with other molecules.

YCR007C is a suspected gene whose biological process, molecular function, and cellular component are unknown. In a previous assignment I hypothesized that since it belongs to the DUP240 gene family, it is likely to have similar functions. The two known genes in this family have as their biological function protein binding, the process they accomplish is vesicle organization, and their cellular components are the golgi apparatus, the endoplasmic reticulum, and the plasma membrane.

3D Structure: Unfortunately, the 3D structure of the YCR007C protein is not known.

Since I found no information for this protein on the Y2H database, this would be the first experiment I would perform on this gene as well. This would confirm the interactions between YCR007C and its two primary nodes, which are as of yet unverified, and would also identify any other proteins that it interacts with.

The structure of this protein also needs to be determined. By using the protein sequence, researchers could make predictions of the 3D structure of the protein, and this would help in predicting the function of the protein. These theories could then be tested experimentally, and we would know if this protein is an important link in the mitotic cell cycle.

Though I am not sure of the experimental design, we need to determine the cellular component of this protein. Knowing where a protein functions is very important in determining its role in the cell.

Creating a knockout strain of this gene would also provide us with information on the function of this protein in the cell. Previous studies found that the cell is viable with the deletion of this gene, but it would be interesting to determine whether it is able to go through mitosis. If the prediction above is correct and YCR007C has a role in mitosis, these cells would not be able to divide mitotically, even if they were able to mate successfully.