The Gene to Cure HIV: APOBEC3G (aka CEM15)

"This web page was produced as an assignment for an undergraduate course at Davidson College."

(NCBI 2006)

General Information(NCBI 2006, UniProt 2006, Sheehy et al. 2002)

  • Gene name: APOBEC3G
  • Also called CEM15, ARP9, MDS019, FLJ12740, bK150C2.7, dJ494G10.1
  • Part of a group of genes related to APOBEC1, a C to U RNA-editing cytidine deaminase
  • Located on chromosome 22 with other APOBEC genes and pseudo genes
  • Suppressed by virion infectivity factor (Vif) during HIV infection
  • Protein generated is an mRNA editing enzyme and catalytic polypeptide
  • Also binds zinc ions, other metal ions, and proteins
  • Protein inhibits HIV infectivity
  • Protein has a C-terminal sequence similar to apobec-1 (50%) and phorbolin-1 (70%), whose C-terminals are essential for catalytic function
  • Found in spleen, testes, ovary and peripheral blood leukocytes, CD4+ lymphocytes

    The Gene Discovery

    Scientific Literature vs Popular Sources

    Categories covered by the Articles:

    In 2002 an article was published in Nature by Sheehy, et al. called The isolation of a human gene that inhibits HIV-1 (human immunodeficiency virus 1) infection and is suppressed by the viral Vif protein. This paper introduced the discovery of the gene CEM15 and its potential in fighting HIV-1. The content and presentation of this article can be broken up into five main categories: why they started looking, how they identified the possible gene, how they tested the gene to support their identification, how they characterized the gene, and what possible mechanisms are run by the gene in order to suppress HIV-1.

    The 2002 Times, London article "Scientists find gene that fights deadly HIV: Discovery provides new target for AIDS treatments" covers the discovery of the gene in contexts both similar to and different than the scientific publication: what was discovered, why this seems especially relevant, how they found the gene's function, and the basic workings of the virus it fights.

    Categories that seem both similar and different:


    Missing Links (Categories that do not cross):

    Related Genes

    When the paper was first written it was unknown whether CEM15 made up only part or all of this antiviral system. The possibility that other genes could be contributing to the antiviral activity is acknowledged, but did not have enough support to say if any genes really were or what they are (Sheehy et al. 2002).

  • APOBEC3F is coexpressed with CEM15 (APOBEC3G). The two generated proteins join together to produce heterodimers (Wiegand et al. 2004).
  • The genes that produce the proteins Cul5, elongins B and C, and Rbx1 need to be expressed for Vif to suppress CEM15 ( Yu et al. 2003).
  • Links

    Erin's Genomics Web page
    Genomics Course Page
    Biology Home Page

    This page was created by Erin Zwack. Comments and questions are welcome.